Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22310 and BWI76_RS22315 overlap by 1 nucleotides BWI76_RS22315 and BWI76_RS22320 are separated by 9 nucleotides BWI76_RS22320 and BWI76_RS22325 are separated by 17 nucleotides BWI76_RS22325 and BWI76_RS22330 are separated by 2 nucleotides
BWI76_RS22310: BWI76_RS22310 - hydrogenase 3 small subunit, at 4,650,832 to 4,651,599
_RS22310
BWI76_RS22315: BWI76_RS22315 - formate hydrogenlyase complex iron-sulfur subunit, at 4,651,599 to 4,652,141
_RS22315
BWI76_RS22320: BWI76_RS22320 - hydrogenase 3 large subunit, at 4,652,151 to 4,653,860
_RS22320
BWI76_RS22325: BWI76_RS22325 - hydrogenase 3 membrane subunit, at 4,653,878 to 4,654,801
_RS22325
BWI76_RS22330: BWI76_RS22330 - formate hydrogenlyase subunit 3, at 4,654,804 to 4,656,630
_RS22330
Position (kb) 4652
4653
4654 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4651.316 kb on + strand, within BWI76_RS22310 at 4651.334 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.414 kb on - strand, within BWI76_RS22310 at 4651.518 kb on + strand, within BWI76_RS22310 at 4651.620 kb on - strand at 4651.646 kb on + strand at 4651.656 kb on + strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4651.664 kb on - strand, within BWI76_RS22315 at 4652.005 kb on - strand, within BWI76_RS22315 at 4652.078 kb on + strand, within BWI76_RS22315 at 4652.102 kb on - strand at 4652.190 kb on + strand at 4652.212 kb on + strand at 4652.234 kb on + strand at 4652.257 kb on + strand at 4652.257 kb on + strand at 4652.265 kb on - strand at 4652.281 kb on - strand at 4652.309 kb on + strand at 4652.392 kb on - strand, within BWI76_RS22320 at 4652.465 kb on + strand, within BWI76_RS22320 at 4652.472 kb on + strand, within BWI76_RS22320 at 4652.473 kb on - strand, within BWI76_RS22320 at 4652.542 kb on + strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.550 kb on - strand, within BWI76_RS22320 at 4652.597 kb on - strand, within BWI76_RS22320 at 4652.794 kb on - strand, within BWI76_RS22320 at 4652.884 kb on - strand, within BWI76_RS22320 at 4652.932 kb on - strand, within BWI76_RS22320 at 4653.168 kb on + strand, within BWI76_RS22320 at 4653.222 kb on - strand, within BWI76_RS22320 at 4653.289 kb on + strand, within BWI76_RS22320 at 4653.314 kb on + strand, within BWI76_RS22320 at 4653.327 kb on - strand, within BWI76_RS22320 at 4653.353 kb on + strand, within BWI76_RS22320 at 4653.581 kb on + strand, within BWI76_RS22320 at 4653.607 kb on + strand, within BWI76_RS22320 at 4653.779 kb on + strand at 4653.956 kb on - strand at 4654.110 kb on + strand, within BWI76_RS22325 at 4654.128 kb on - strand, within BWI76_RS22325 at 4654.312 kb on - strand, within BWI76_RS22325 at 4654.328 kb on + strand, within BWI76_RS22325 at 4654.367 kb on - strand, within BWI76_RS22325 at 4654.576 kb on + strand, within BWI76_RS22325
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 4,651,316 + BWI76_RS22310 0.63 -0.8 4,651,334 - BWI76_RS22310 0.65 -0.0 4,651,414 - BWI76_RS22310 0.76 +0.5 4,651,414 - BWI76_RS22310 0.76 +0.2 4,651,518 + BWI76_RS22310 0.89 -0.8 4,651,620 - -0.6 4,651,646 + -0.2 4,651,656 + BWI76_RS22315 0.10 -0.5 4,651,664 - BWI76_RS22315 0.12 +0.1 4,651,664 - BWI76_RS22315 0.12 -0.9 4,652,005 - BWI76_RS22315 0.75 -0.5 4,652,078 + BWI76_RS22315 0.88 +0.3 4,652,102 - -0.1 4,652,190 + +1.4 4,652,212 + +0.2 4,652,234 + -0.3 4,652,257 + -1.9 4,652,257 + +0.2 4,652,265 - +0.4 4,652,281 - +0.3 4,652,309 + +0.2 4,652,392 - BWI76_RS22320 0.14 +0.1 4,652,465 + BWI76_RS22320 0.18 -1.3 4,652,472 + BWI76_RS22320 0.19 -0.2 4,652,473 - BWI76_RS22320 0.19 -0.8 4,652,542 + BWI76_RS22320 0.23 -0.2 4,652,550 - BWI76_RS22320 0.23 +0.0 4,652,550 - BWI76_RS22320 0.23 +0.6 4,652,597 - BWI76_RS22320 0.26 +0.0 4,652,794 - BWI76_RS22320 0.38 -1.2 4,652,884 - BWI76_RS22320 0.43 -1.0 4,652,932 - BWI76_RS22320 0.46 +0.7 4,653,168 + BWI76_RS22320 0.59 -0.2 4,653,222 - BWI76_RS22320 0.63 +0.5 4,653,289 + BWI76_RS22320 0.67 -1.2 4,653,314 + BWI76_RS22320 0.68 -0.1 4,653,327 - BWI76_RS22320 0.69 +0.2 4,653,353 + BWI76_RS22320 0.70 -0.4 4,653,581 + BWI76_RS22320 0.84 +0.0 4,653,607 + BWI76_RS22320 0.85 -0.5 4,653,779 + -0.1 4,653,956 - +0.1 4,654,110 + BWI76_RS22325 0.25 +0.0 4,654,128 - BWI76_RS22325 0.27 -0.5 4,654,312 - BWI76_RS22325 0.47 -0.3 4,654,328 + BWI76_RS22325 0.49 +1.8 4,654,367 - BWI76_RS22325 0.53 -0.8 4,654,576 + BWI76_RS22325 0.76 +0.5
Or see this region's nucleotide sequence