Experiment: Sucrose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02020 and PGA1_c02030 are separated by 218 nucleotides PGA1_c02030 and PGA1_c02040 overlap by 4 nucleotides PGA1_c02040 and PGA1_c02050 are separated by 199 nucleotides
PGA1_c02020: PGA1_c02020 - Predicted metal-binding protein, at 185,235 to 185,693
_c02020
PGA1_c02030: PGA1_c02030 - phosphoglycerate mutase-like protein, at 185,912 to 186,409
_c02030
PGA1_c02040: PGA1_c02040 - hypothetical protein, at 186,406 to 187,149
_c02040
PGA1_c02050: PGA1_c02050 - acetylglutamate kinase ArgB, at 187,349 to 188,209
_c02050
Position (kb) 186
187
188 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on - strand, within PGA1_c02020 at 185.446 kb on - strand, within PGA1_c02020 at 185.537 kb on + strand, within PGA1_c02020 at 185.545 kb on - strand, within PGA1_c02020 at 185.553 kb on - strand, within PGA1_c02020 at 185.688 kb on + strand at 185.717 kb on + strand at 185.717 kb on + strand at 185.725 kb on - strand at 185.736 kb on - strand at 185.911 kb on - strand at 185.913 kb on - strand at 186.010 kb on + strand, within PGA1_c02030 at 186.124 kb on + strand, within PGA1_c02030 at 186.246 kb on - strand, within PGA1_c02030 at 186.353 kb on + strand, within PGA1_c02030 at 186.391 kb on + strand at 186.457 kb on + strand at 186.527 kb on + strand, within PGA1_c02040 at 186.535 kb on - strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.625 kb on + strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.656 kb on - strand, within PGA1_c02040 at 186.769 kb on - strand, within PGA1_c02040 at 186.779 kb on - strand, within PGA1_c02040 at 186.871 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.903 kb on + strand, within PGA1_c02040 at 186.997 kb on - strand, within PGA1_c02040 at 186.999 kb on + strand, within PGA1_c02040 at 187.005 kb on + strand, within PGA1_c02040 at 187.007 kb on + strand, within PGA1_c02040 at 187.013 kb on - strand, within PGA1_c02040 at 187.144 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on + strand at 187.193 kb on - strand at 187.225 kb on + strand at 187.244 kb on - strand at 187.347 kb on + strand at 187.355 kb on - strand at 187.355 kb on - strand at 187.446 kb on + strand, within PGA1_c02050 at 187.454 kb on - strand, within PGA1_c02050 at 187.707 kb on + strand, within PGA1_c02050 at 187.707 kb on + strand, within PGA1_c02050 at 187.772 kb on + strand, within PGA1_c02050 at 187.897 kb on - strand, within PGA1_c02050 at 188.020 kb on + strand, within PGA1_c02050 at 188.028 kb on - strand, within PGA1_c02050 at 188.033 kb on - strand, within PGA1_c02050 at 188.064 kb on + strand, within PGA1_c02050
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) remove 185,438 + PGA1_c02020 0.44 +1.6 185,438 + PGA1_c02020 0.44 -0.6 185,438 - PGA1_c02020 0.44 -1.6 185,446 - PGA1_c02020 0.46 -1.7 185,537 + PGA1_c02020 0.66 +0.4 185,545 - PGA1_c02020 0.68 +0.8 185,553 - PGA1_c02020 0.69 +1.8 185,688 + +1.2 185,717 + -0.4 185,717 + +1.1 185,725 - -0.4 185,736 - -1.7 185,911 - +2.0 185,913 - -0.1 186,010 + PGA1_c02030 0.20 -0.4 186,124 + PGA1_c02030 0.43 +1.2 186,246 - PGA1_c02030 0.67 -1.5 186,353 + PGA1_c02030 0.89 +1.3 186,391 + -1.1 186,457 + -0.6 186,527 + PGA1_c02040 0.16 -1.8 186,535 - PGA1_c02040 0.17 -0.8 186,610 + PGA1_c02040 0.27 +0.2 186,610 + PGA1_c02040 0.27 -2.6 186,610 + PGA1_c02040 0.27 -1.1 186,618 - PGA1_c02040 0.28 -1.5 186,618 - PGA1_c02040 0.28 -1.1 186,618 - PGA1_c02040 0.28 +0.5 186,625 + PGA1_c02040 0.29 -0.1 186,651 - PGA1_c02040 0.33 +1.0 186,651 - PGA1_c02040 0.33 -1.1 186,656 - PGA1_c02040 0.34 -0.8 186,769 - PGA1_c02040 0.49 +0.0 186,779 - PGA1_c02040 0.50 -0.6 186,871 + PGA1_c02040 0.62 -0.2 186,903 + PGA1_c02040 0.67 +1.1 186,903 + PGA1_c02040 0.67 +1.8 186,997 - PGA1_c02040 0.79 -1.4 186,999 + PGA1_c02040 0.80 -0.9 187,005 + PGA1_c02040 0.81 -1.7 187,007 + PGA1_c02040 0.81 -0.4 187,013 - PGA1_c02040 0.82 -0.3 187,144 + -0.9 187,193 + +0.8 187,193 + +0.4 187,193 + +0.3 187,193 - -1.1 187,225 + +1.1 187,244 - -0.2 187,347 + +0.2 187,355 - +0.6 187,355 - +0.3 187,446 + PGA1_c02050 0.11 +0.8 187,454 - PGA1_c02050 0.12 -0.8 187,707 + PGA1_c02050 0.42 -0.2 187,707 + PGA1_c02050 0.42 -0.2 187,772 + PGA1_c02050 0.49 +0.8 187,897 - PGA1_c02050 0.64 +1.8 188,020 + PGA1_c02050 0.78 -0.2 188,028 - PGA1_c02050 0.79 -0.2 188,033 - PGA1_c02050 0.79 +0.8 188,064 + PGA1_c02050 0.83 -1.5
Or see this region's nucleotide sequence