Experiment: malate (C) and Ammonium chloride (N); with TAPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS30525 and PFLU_RS30530 overlap by 4 nucleotides PFLU_RS30530 and PFLU_RS08180 are separated by 3 nucleotides PFLU_RS08180 and PFLU_RS08185 are separated by 48 nucleotides
PFLU_RS30525: PFLU_RS30525 - SDR family oxidoreductase, at 1,827,250 to 1,828,008
_RS30525
PFLU_RS30530: PFLU_RS30530 - acetyltransferase, at 1,828,005 to 1,828,670
_RS30530
PFLU_RS08180: PFLU_RS08180 - ketoacyl-ACP synthase III, at 1,828,674 to 1,829,690
_RS08180
PFLU_RS08185: PFLU_RS08185 - DegT/DnrJ/EryC1/StrS aminotransferase family protein, at 1,829,739 to 1,830,917
_RS08185
Position (kb)
1828
1829
1830 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1827.773 kb on + strand, within PFLU_RS30525 at 1827.773 kb on + strand, within PFLU_RS30525 at 1827.773 kb on + strand, within PFLU_RS30525 at 1827.774 kb on - strand, within PFLU_RS30525 at 1827.774 kb on - strand, within PFLU_RS30525 at 1827.774 kb on - strand, within PFLU_RS30525 at 1827.774 kb on - strand, within PFLU_RS30525 at 1827.786 kb on + strand, within PFLU_RS30525 at 1827.786 kb on + strand, within PFLU_RS30525 at 1827.786 kb on + strand, within PFLU_RS30525 at 1827.787 kb on - strand, within PFLU_RS30525 at 1827.796 kb on - strand, within PFLU_RS30525 at 1827.837 kb on + strand, within PFLU_RS30525 at 1827.838 kb on - strand, within PFLU_RS30525 at 1827.852 kb on - strand, within PFLU_RS30525 at 1827.875 kb on + strand, within PFLU_RS30525 at 1827.876 kb on - strand, within PFLU_RS30525 at 1827.896 kb on + strand, within PFLU_RS30525 at 1827.920 kb on + strand, within PFLU_RS30525 at 1827.920 kb on + strand, within PFLU_RS30525 at 1827.920 kb on + strand, within PFLU_RS30525 at 1827.920 kb on + strand, within PFLU_RS30525 at 1827.921 kb on - strand, within PFLU_RS30525 at 1827.921 kb on - strand, within PFLU_RS30525 at 1827.925 kb on + strand, within PFLU_RS30525 at 1827.926 kb on - strand, within PFLU_RS30525 at 1827.926 kb on - strand, within PFLU_RS30525 at 1827.933 kb on + strand at 1827.934 kb on - strand at 1827.934 kb on - strand at 1827.937 kb on + strand at 1827.937 kb on + strand at 1827.937 kb on + strand at 1827.937 kb on + strand at 1827.937 kb on + strand at 1827.937 kb on + strand at 1827.938 kb on - strand at 1827.938 kb on - strand at 1827.938 kb on - strand at 1827.972 kb on + strand at 1827.980 kb on + strand at 1827.980 kb on + strand at 1827.981 kb on - strand at 1827.997 kb on + strand at 1827.998 kb on - strand at 1828.024 kb on + strand at 1828.028 kb on - strand at 1828.100 kb on + strand, within PFLU_RS30530 at 1828.100 kb on + strand, within PFLU_RS30530 at 1828.100 kb on + strand, within PFLU_RS30530 at 1828.101 kb on - strand, within PFLU_RS30530 at 1828.101 kb on - strand, within PFLU_RS30530 at 1828.101 kb on - strand, within PFLU_RS30530 at 1828.106 kb on + strand, within PFLU_RS30530 at 1828.106 kb on + strand, within PFLU_RS30530 at 1828.107 kb on - strand, within PFLU_RS30530 at 1828.107 kb on - strand, within PFLU_RS30530 at 1828.119 kb on - strand, within PFLU_RS30530 at 1828.155 kb on - strand, within PFLU_RS30530 at 1828.187 kb on + strand, within PFLU_RS30530 at 1828.188 kb on - strand, within PFLU_RS30530 at 1828.227 kb on - strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.230 kb on + strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.231 kb on - strand, within PFLU_RS30530 at 1828.238 kb on - strand, within PFLU_RS30530 at 1828.426 kb on + strand, within PFLU_RS30530 at 1828.440 kb on - strand, within PFLU_RS30530 at 1828.476 kb on - strand, within PFLU_RS30530 at 1828.499 kb on + strand, within PFLU_RS30530 at 1828.499 kb on + strand, within PFLU_RS30530 at 1828.559 kb on + strand, within PFLU_RS30530 at 1828.560 kb on - strand, within PFLU_RS30530 at 1828.604 kb on - strand at 1828.604 kb on - strand at 1828.613 kb on + strand at 1828.700 kb on + strand at 1828.714 kb on + strand at 1828.725 kb on - strand at 1828.725 kb on - strand at 1828.771 kb on + strand at 1828.911 kb on - strand, within PFLU_RS08180 at 1828.915 kb on + strand, within PFLU_RS08180 at 1828.935 kb on + strand, within PFLU_RS08180 at 1828.935 kb on + strand, within PFLU_RS08180 at 1828.958 kb on + strand, within PFLU_RS08180 at 1828.959 kb on - strand, within PFLU_RS08180 at 1828.959 kb on - strand, within PFLU_RS08180 at 1828.986 kb on - strand, within PFLU_RS08180 at 1829.060 kb on - strand, within PFLU_RS08180 at 1829.086 kb on + strand, within PFLU_RS08180 at 1829.087 kb on - strand, within PFLU_RS08180 at 1829.111 kb on + strand, within PFLU_RS08180 at 1829.114 kb on + strand, within PFLU_RS08180 at 1829.118 kb on + strand, within PFLU_RS08180 at 1829.119 kb on - strand, within PFLU_RS08180 at 1829.148 kb on - strand, within PFLU_RS08180 at 1829.194 kb on + strand, within PFLU_RS08180 at 1829.194 kb on + strand, within PFLU_RS08180 at 1829.207 kb on + strand, within PFLU_RS08180 at 1829.339 kb on + strand, within PFLU_RS08180 at 1829.348 kb on + strand, within PFLU_RS08180 at 1829.475 kb on - strand, within PFLU_RS08180 at 1829.510 kb on - strand, within PFLU_RS08180 at 1829.564 kb on - strand, within PFLU_RS08180 at 1829.681 kb on + strand at 1829.724 kb on + strand at 1829.724 kb on + strand at 1829.725 kb on - strand at 1829.747 kb on + strand at 1829.807 kb on + strand at 1829.808 kb on - strand at 1829.819 kb on + strand at 1829.819 kb on + strand at 1829.829 kb on + strand at 1829.915 kb on + strand, within PFLU_RS08185 at 1829.933 kb on - strand, within PFLU_RS08185 at 1829.978 kb on + strand, within PFLU_RS08185 at 1830.011 kb on + strand, within PFLU_RS08185 at 1830.016 kb on + strand, within PFLU_RS08185 at 1830.016 kb on + strand, within PFLU_RS08185 at 1830.016 kb on + strand, within PFLU_RS08185 at 1830.037 kb on + strand, within PFLU_RS08185 at 1830.038 kb on - strand, within PFLU_RS08185 at 1830.038 kb on - strand, within PFLU_RS08185 at 1830.073 kb on + strand, within PFLU_RS08185 at 1830.074 kb on - strand, within PFLU_RS08185 at 1830.111 kb on - strand, within PFLU_RS08185 at 1830.111 kb on - strand, within PFLU_RS08185 at 1830.191 kb on + strand, within PFLU_RS08185 at 1830.227 kb on + strand, within PFLU_RS08185 at 1830.228 kb on - strand, within PFLU_RS08185 at 1830.228 kb on - strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.231 kb on + strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.232 kb on - strand, within PFLU_RS08185 at 1830.248 kb on - strand, within PFLU_RS08185 at 1830.248 kb on - strand, within PFLU_RS08185 at 1830.290 kb on + strand, within PFLU_RS08185 at 1830.290 kb on + strand, within PFLU_RS08185 at 1830.291 kb on - strand, within PFLU_RS08185 at 1830.291 kb on - strand, within PFLU_RS08185 at 1830.291 kb on - strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.366 kb on + strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.367 kb on - strand, within PFLU_RS08185 at 1830.368 kb on + strand, within PFLU_RS08185 at 1830.368 kb on + strand, within PFLU_RS08185 at 1830.368 kb on + strand, within PFLU_RS08185 at 1830.399 kb on + strand, within PFLU_RS08185 at 1830.400 kb on - strand, within PFLU_RS08185 at 1830.400 kb on - strand, within PFLU_RS08185 at 1830.400 kb on - strand, within PFLU_RS08185 at 1830.400 kb on - strand, within PFLU_RS08185 at 1830.484 kb on + strand, within PFLU_RS08185 at 1830.492 kb on - strand, within PFLU_RS08185 at 1830.506 kb on + strand, within PFLU_RS08185 at 1830.506 kb on + strand, within PFLU_RS08185 at 1830.506 kb on + strand, within PFLU_RS08185 at 1830.523 kb on + strand, within PFLU_RS08185 at 1830.524 kb on - strand, within PFLU_RS08185 at 1830.524 kb on - strand, within PFLU_RS08185 at 1830.602 kb on + strand, within PFLU_RS08185
Per-strain Table
Position Strand Gene LocusTag Fraction malate (C) and Ammonium chloride (N); with TAPS remove 1,827,773 + PFLU_RS30525 0.69 +0.3 1,827,773 + PFLU_RS30525 0.69 -0.8 1,827,773 + PFLU_RS30525 0.69 -1.8 1,827,774 - PFLU_RS30525 0.69 -0.6 1,827,774 - PFLU_RS30525 0.69 -1.3 1,827,774 - PFLU_RS30525 0.69 -2.2 1,827,774 - PFLU_RS30525 0.69 -2.8 1,827,786 + PFLU_RS30525 0.71 -1.6 1,827,786 + PFLU_RS30525 0.71 -2.7 1,827,786 + PFLU_RS30525 0.71 -3.6 1,827,787 - PFLU_RS30525 0.71 -1.9 1,827,796 - PFLU_RS30525 0.72 -2.8 1,827,837 + PFLU_RS30525 0.77 -1.7 1,827,838 - PFLU_RS30525 0.77 -2.4 1,827,852 - PFLU_RS30525 0.79 -2.6 1,827,875 + PFLU_RS30525 0.82 -2.9 1,827,876 - PFLU_RS30525 0.82 -3.0 1,827,896 + PFLU_RS30525 0.85 -2.2 1,827,920 + PFLU_RS30525 0.88 -1.8 1,827,920 + PFLU_RS30525 0.88 -1.6 1,827,920 + PFLU_RS30525 0.88 -1.4 1,827,920 + PFLU_RS30525 0.88 -1.4 1,827,921 - PFLU_RS30525 0.88 -3.7 1,827,921 - PFLU_RS30525 0.88 -0.3 1,827,925 + PFLU_RS30525 0.89 -3.6 1,827,926 - PFLU_RS30525 0.89 -2.7 1,827,926 - PFLU_RS30525 0.89 -1.9 1,827,933 + -2.8 1,827,934 - -1.6 1,827,934 - -1.1 1,827,937 + -0.4 1,827,937 + -1.9 1,827,937 + -1.5 1,827,937 + -1.5 1,827,937 + -1.4 1,827,937 + -1.6 1,827,938 - -2.4 1,827,938 - -2.6 1,827,938 - -1.4 1,827,972 + -3.3 1,827,980 + -1.5 1,827,980 + -2.9 1,827,981 - -2.0 1,827,997 + -2.8 1,827,998 - +0.1 1,828,024 + -2.4 1,828,028 - -2.4 1,828,100 + PFLU_RS30530 0.14 -1.4 1,828,100 + PFLU_RS30530 0.14 -2.4 1,828,100 + PFLU_RS30530 0.14 -3.0 1,828,101 - PFLU_RS30530 0.14 -0.5 1,828,101 - PFLU_RS30530 0.14 -2.9 1,828,101 - PFLU_RS30530 0.14 -0.6 1,828,106 + PFLU_RS30530 0.15 -1.5 1,828,106 + PFLU_RS30530 0.15 -1.5 1,828,107 - PFLU_RS30530 0.15 -1.8 1,828,107 - PFLU_RS30530 0.15 -2.9 1,828,119 - PFLU_RS30530 0.17 -0.6 1,828,155 - PFLU_RS30530 0.23 -0.6 1,828,187 + PFLU_RS30530 0.27 -2.5 1,828,188 - PFLU_RS30530 0.27 -2.0 1,828,227 - PFLU_RS30530 0.33 -1.9 1,828,230 + PFLU_RS30530 0.34 -1.6 1,828,230 + PFLU_RS30530 0.34 -2.8 1,828,230 + PFLU_RS30530 0.34 -1.4 1,828,230 + PFLU_RS30530 0.34 -2.9 1,828,230 + PFLU_RS30530 0.34 -1.0 1,828,230 + PFLU_RS30530 0.34 -2.2 1,828,230 + PFLU_RS30530 0.34 -1.8 1,828,230 + PFLU_RS30530 0.34 -1.8 1,828,231 - PFLU_RS30530 0.34 -3.2 1,828,231 - PFLU_RS30530 0.34 -2.0 1,828,231 - PFLU_RS30530 0.34 -1.3 1,828,231 - PFLU_RS30530 0.34 -2.7 1,828,231 - PFLU_RS30530 0.34 +0.1 1,828,231 - PFLU_RS30530 0.34 -2.1 1,828,231 - PFLU_RS30530 0.34 -2.3 1,828,231 - PFLU_RS30530 0.34 -0.8 1,828,231 - PFLU_RS30530 0.34 -3.2 1,828,231 - PFLU_RS30530 0.34 -2.2 1,828,231 - PFLU_RS30530 0.34 -0.3 1,828,231 - PFLU_RS30530 0.34 -1.2 1,828,238 - PFLU_RS30530 0.35 -1.2 1,828,426 + PFLU_RS30530 0.63 -0.4 1,828,440 - PFLU_RS30530 0.65 -1.8 1,828,476 - PFLU_RS30530 0.71 -2.4 1,828,499 + PFLU_RS30530 0.74 -2.5 1,828,499 + PFLU_RS30530 0.74 -1.4 1,828,559 + PFLU_RS30530 0.83 -2.8 1,828,560 - PFLU_RS30530 0.83 -0.4 1,828,604 - -2.0 1,828,604 - -1.4 1,828,613 + -1.2 1,828,700 + -0.5 1,828,714 + -2.0 1,828,725 - +0.4 1,828,725 - -0.4 1,828,771 + -1.3 1,828,911 - PFLU_RS08180 0.23 -0.7 1,828,915 + PFLU_RS08180 0.24 +0.3 1,828,935 + PFLU_RS08180 0.26 -1.7 1,828,935 + PFLU_RS08180 0.26 -1.3 1,828,958 + PFLU_RS08180 0.28 -2.1 1,828,959 - PFLU_RS08180 0.28 -1.4 1,828,959 - PFLU_RS08180 0.28 -2.4 1,828,986 - PFLU_RS08180 0.31 -1.2 1,829,060 - PFLU_RS08180 0.38 -0.8 1,829,086 + PFLU_RS08180 0.41 -2.2 1,829,087 - PFLU_RS08180 0.41 -1.2 1,829,111 + PFLU_RS08180 0.43 -3.6 1,829,114 + PFLU_RS08180 0.43 -2.4 1,829,118 + PFLU_RS08180 0.44 -0.6 1,829,119 - PFLU_RS08180 0.44 -0.7 1,829,148 - PFLU_RS08180 0.47 -0.6 1,829,194 + PFLU_RS08180 0.51 -3.0 1,829,194 + PFLU_RS08180 0.51 -3.2 1,829,207 + PFLU_RS08180 0.52 -2.4 1,829,339 + PFLU_RS08180 0.65 -1.2 1,829,348 + PFLU_RS08180 0.66 -0.8 1,829,475 - PFLU_RS08180 0.79 -1.2 1,829,510 - PFLU_RS08180 0.82 -1.3 1,829,564 - PFLU_RS08180 0.88 +0.1 1,829,681 + -2.6 1,829,724 + +0.3 1,829,724 + -0.2 1,829,725 - +0.8 1,829,747 + -2.0 1,829,807 + -2.5 1,829,808 - -1.7 1,829,819 + -1.0 1,829,819 + -2.7 1,829,829 + -0.8 1,829,915 + PFLU_RS08185 0.15 -1.4 1,829,933 - PFLU_RS08185 0.16 -2.2 1,829,978 + PFLU_RS08185 0.20 -3.1 1,830,011 + PFLU_RS08185 0.23 -1.8 1,830,016 + PFLU_RS08185 0.23 -1.8 1,830,016 + PFLU_RS08185 0.23 -1.8 1,830,016 + PFLU_RS08185 0.23 -2.5 1,830,037 + PFLU_RS08185 0.25 -2.0 1,830,038 - PFLU_RS08185 0.25 -1.4 1,830,038 - PFLU_RS08185 0.25 -1.7 1,830,073 + PFLU_RS08185 0.28 -1.6 1,830,074 - PFLU_RS08185 0.28 -1.0 1,830,111 - PFLU_RS08185 0.32 -0.0 1,830,111 - PFLU_RS08185 0.32 -2.4 1,830,191 + PFLU_RS08185 0.38 -1.5 1,830,227 + PFLU_RS08185 0.41 -1.7 1,830,228 - PFLU_RS08185 0.41 -2.2 1,830,228 - PFLU_RS08185 0.41 -2.1 1,830,231 + PFLU_RS08185 0.42 -2.8 1,830,231 + PFLU_RS08185 0.42 -0.9 1,830,231 + PFLU_RS08185 0.42 -0.5 1,830,231 + PFLU_RS08185 0.42 -2.0 1,830,231 + PFLU_RS08185 0.42 +0.8 1,830,231 + PFLU_RS08185 0.42 -0.7 1,830,231 + PFLU_RS08185 0.42 -1.3 1,830,231 + PFLU_RS08185 0.42 -1.0 1,830,231 + PFLU_RS08185 0.42 -1.9 1,830,231 + PFLU_RS08185 0.42 -1.1 1,830,232 - PFLU_RS08185 0.42 -1.7 1,830,232 - PFLU_RS08185 0.42 -1.3 1,830,232 - PFLU_RS08185 0.42 -1.4 1,830,232 - PFLU_RS08185 0.42 -2.2 1,830,232 - PFLU_RS08185 0.42 -2.9 1,830,232 - PFLU_RS08185 0.42 -1.5 1,830,232 - PFLU_RS08185 0.42 -2.3 1,830,232 - PFLU_RS08185 0.42 -1.1 1,830,232 - PFLU_RS08185 0.42 -2.8 1,830,232 - PFLU_RS08185 0.42 -2.3 1,830,232 - PFLU_RS08185 0.42 -3.6 1,830,248 - PFLU_RS08185 0.43 -1.6 1,830,248 - PFLU_RS08185 0.43 -2.5 1,830,290 + PFLU_RS08185 0.47 +0.3 1,830,290 + PFLU_RS08185 0.47 -0.9 1,830,291 - PFLU_RS08185 0.47 -0.4 1,830,291 - PFLU_RS08185 0.47 -2.1 1,830,291 - PFLU_RS08185 0.47 -0.6 1,830,366 + PFLU_RS08185 0.53 -0.7 1,830,366 + PFLU_RS08185 0.53 -2.5 1,830,366 + PFLU_RS08185 0.53 -0.9 1,830,366 + PFLU_RS08185 0.53 -1.6 1,830,366 + PFLU_RS08185 0.53 -3.2 1,830,366 + PFLU_RS08185 0.53 -1.3 1,830,367 - PFLU_RS08185 0.53 -2.7 1,830,367 - PFLU_RS08185 0.53 -1.6 1,830,367 - PFLU_RS08185 0.53 -3.3 1,830,367 - PFLU_RS08185 0.53 -2.8 1,830,367 - PFLU_RS08185 0.53 -3.1 1,830,367 - PFLU_RS08185 0.53 -0.7 1,830,367 - PFLU_RS08185 0.53 -1.7 1,830,368 + PFLU_RS08185 0.53 -1.9 1,830,368 + PFLU_RS08185 0.53 -0.8 1,830,368 + PFLU_RS08185 0.53 -1.4 1,830,399 + PFLU_RS08185 0.56 -2.0 1,830,400 - PFLU_RS08185 0.56 -1.7 1,830,400 - PFLU_RS08185 0.56 -1.3 1,830,400 - PFLU_RS08185 0.56 +0.5 1,830,400 - PFLU_RS08185 0.56 -1.7 1,830,484 + PFLU_RS08185 0.63 -0.6 1,830,492 - PFLU_RS08185 0.64 -1.9 1,830,506 + PFLU_RS08185 0.65 -2.5 1,830,506 + PFLU_RS08185 0.65 -2.0 1,830,506 + PFLU_RS08185 0.65 -2.3 1,830,523 + PFLU_RS08185 0.66 -1.1 1,830,524 - PFLU_RS08185 0.67 -1.8 1,830,524 - PFLU_RS08185 0.67 -1.8 1,830,602 + PFLU_RS08185 0.73 -1.0
Or see this region's nucleotide sequence