Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04460 and BWI76_RS04465 are separated by 457 nucleotides BWI76_RS04465 and BWI76_RS04470 are separated by 18 nucleotides
BWI76_RS04460: BWI76_RS04460 - 4-hydroxy-tetrahydrodipicolinate reductase, at 921,267 to 922,088
_RS04460
BWI76_RS04465: BWI76_RS04465 - carbamoyl-phosphate synthase small subunit, at 922,546 to 923,694
_RS04465
BWI76_RS04470: BWI76_RS04470 - carbamoyl phosphate synthase large subunit, at 923,713 to 926,937
_RS04470
Position (kb) 922
923
924 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 922.434 kb on + strand at 922.440 kb on - strand at 922.440 kb on - strand at 922.599 kb on + strand at 922.828 kb on + strand, within BWI76_RS04465 at 922.836 kb on + strand, within BWI76_RS04465 at 922.836 kb on - strand, within BWI76_RS04465 at 922.897 kb on - strand, within BWI76_RS04465 at 923.052 kb on - strand, within BWI76_RS04465 at 923.206 kb on + strand, within BWI76_RS04465 at 923.623 kb on - strand at 923.628 kb on - strand at 923.794 kb on - strand at 923.808 kb on + strand at 923.816 kb on - strand at 923.855 kb on + strand at 923.862 kb on + strand at 923.879 kb on - strand at 923.995 kb on + strand at 923.995 kb on + strand at 924.003 kb on - strand at 924.256 kb on + strand, within BWI76_RS04470 at 924.264 kb on - strand at 924.575 kb on - strand, within BWI76_RS04470
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 922,434 + -0.5 922,440 - +1.0 922,440 - +1.2 922,599 + -3.4 922,828 + BWI76_RS04465 0.25 -4.4 922,836 + BWI76_RS04465 0.25 -4.7 922,836 - BWI76_RS04465 0.25 -3.5 922,897 - BWI76_RS04465 0.31 +0.2 923,052 - BWI76_RS04465 0.44 -3.8 923,206 + BWI76_RS04465 0.57 -1.6 923,623 - -2.3 923,628 - -3.6 923,794 - -2.9 923,808 + -2.9 923,816 - +1.2 923,855 + -2.2 923,862 + -4.8 923,879 - -4.5 923,995 + -2.0 923,995 + +1.2 924,003 - -1.7 924,256 + BWI76_RS04470 0.17 -2.6 924,264 - -1.2 924,575 - BWI76_RS04470 0.27 -3.5
Or see this region's nucleotide sequence