Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemB-2 and SO4209 are separated by 240 nucleotides SO4209 and SO4210 are separated by 187 nucleotides SO4210 and Sp5SG are separated by 151 nucleotides Sp5SG and Sp23SG are separated by 203 nucleotides
SO4208: hemB-2 - delta-aminolevulinic acid dehydratase (NCBI ptt file), at 4,374,591 to 4,375,601
hemB-2
SO4209: SO4209 - conserved hypothetical protein (NCBI ptt file), at 4,375,842 to 4,376,090
SO4209
SO4210: SO4210 - hypothetical protein (NCBI ptt file), at 4,376,278 to 4,376,823
SO4210
SO_r016: Sp5SG - 5S ribosomal RNA (RefSeq), at 4,376,975 to 4,377,094
Sp5SG
SO_r017: Sp23SG - 23S ribosomal RNA (RefSeq), at 4,377,298 to 4,380,190
Sp23SG
Position (kb)
4376
4377 Strain fitness (log2 ratio)
-2
-1
0
1 at 4375.321 kb on - strand, within hemB-2 at 4375.355 kb on - strand, within hemB-2 at 4375.379 kb on + strand, within hemB-2 at 4375.416 kb on + strand, within hemB-2 at 4375.515 kb on + strand at 4375.645 kb on - strand at 4375.716 kb on - strand at 4375.833 kb on + strand at 4375.841 kb on - strand at 4376.043 kb on - strand, within SO4209 at 4376.482 kb on + strand, within SO4210 at 4376.486 kb on + strand, within SO4210 at 4376.654 kb on + strand, within SO4210
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 4,375,321 - hemB-2 SO4208 0.72 -1.0 4,375,355 - hemB-2 SO4208 0.76 +0.5 4,375,379 + hemB-2 SO4208 0.78 -0.6 4,375,416 + hemB-2 SO4208 0.82 -2.4 4,375,515 + -2.1 4,375,645 - +0.9 4,375,716 - +1.5 4,375,833 + +0.9 4,375,841 - -0.4 4,376,043 - SO4209 0.81 -1.7 4,376,482 + SO4210 0.37 -1.9 4,376,486 + SO4210 0.38 -0.1 4,376,654 + SO4210 0.69 +0.9
Or see this region's nucleotide sequence