Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2275 and SO2276 overlap by 11 nucleotides SO2276 and ibpA are separated by 214 nucleotides ibpA and ilvH are separated by 107 nucleotides ilvH and ilvI overlap by 1 nucleotides
SO2275: SO2275 - ISSod10, transposase OrfA (NCBI ptt file), at 2,387,572 to 2,388,051
SO2275
SO2276: SO2276 - ISSod10, transposase OrfB (NCBI ptt file), at 2,388,041 to 2,388,598
SO2276
SO2277: ibpA - 16 kDa heat shock protein A (NCBI ptt file), at 2,388,813 to 2,389,256
ibpA
SO2278: ilvH - acetolactate synthase III, small subunit (NCBI ptt file), at 2,389,364 to 2,389,858
ilvH
SO2279: ilvI - acetolactate synthase III, large subunit (NCBI ptt file), at 2,389,858 to 2,391,576
ilvI
Position (kb)
2388
2389
2390 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2388.803 kb on + strand at 2388.804 kb on + strand at 2388.865 kb on + strand, within ibpA at 2389.028 kb on + strand, within ibpA at 2389.059 kb on - strand, within ibpA at 2389.104 kb on - strand, within ibpA at 2389.108 kb on + strand, within ibpA at 2389.245 kb on + strand at 2389.275 kb on + strand at 2389.399 kb on - strand at 2389.412 kb on - strand at 2389.587 kb on + strand, within ilvH at 2389.864 kb on - strand at 2390.116 kb on - strand, within ilvI at 2390.116 kb on - strand, within ilvI at 2390.121 kb on - strand, within ilvI at 2390.185 kb on + strand, within ilvI
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 2,388,803 + +1.1 2,388,804 + +1.0 2,388,865 + ibpA SO2277 0.12 -2.4 2,389,028 + ibpA SO2277 0.48 +1.0 2,389,059 - ibpA SO2277 0.55 -0.7 2,389,104 - ibpA SO2277 0.66 -1.6 2,389,108 + ibpA SO2277 0.66 -3.2 2,389,245 + -3.6 2,389,275 + +0.1 2,389,399 - +1.9 2,389,412 - +0.7 2,389,587 + ilvH SO2278 0.45 +1.9 2,389,864 - +0.4 2,390,116 - ilvI SO2279 0.15 +1.8 2,390,116 - ilvI SO2279 0.15 -0.4 2,390,121 - ilvI SO2279 0.15 -1.8 2,390,185 + ilvI SO2279 0.19 -0.7
Or see this region's nucleotide sequence