Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt htpG and SO2017 are separated by 119 nucleotides SO2017 and adk are separated by 140 nucleotides adk and hemH-1 are separated by 79 nucleotides
SO2016: htpG - heat shock protein HtpG (NCBI ptt file), at 2,114,424 to 2,116,337
htpG
SO2017: SO2017 - hypothetical thioredoxin-like protein (NCBI ptt file), at 2,116,457 to 2,117,320
SO2017
SO2018: adk - adenylate kinase (NCBI ptt file), at 2,117,461 to 2,118,105
adk
SO2019: hemH-1 - ferrochelatase (NCBI ptt file), at 2,118,185 to 2,119,231
hemH-1
Position (kb)
2116
2117
2118 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2115.760 kb on + strand, within htpG at 2115.995 kb on + strand, within htpG at 2116.414 kb on - strand at 2116.748 kb on + strand, within SO2017 at 2116.748 kb on + strand, within SO2017 at 2116.826 kb on + strand, within SO2017 at 2116.834 kb on - strand, within SO2017 at 2116.891 kb on - strand, within SO2017 at 2116.972 kb on - strand, within SO2017 at 2117.143 kb on + strand, within SO2017 at 2117.143 kb on + strand, within SO2017 at 2117.151 kb on - strand, within SO2017 at 2117.168 kb on + strand, within SO2017 at 2117.177 kb on + strand, within SO2017 at 2117.299 kb on + strand at 2117.300 kb on - strand at 2117.307 kb on - strand at 2118.092 kb on - strand at 2118.153 kb on + strand at 2118.201 kb on + strand at 2118.289 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 2,115,760 + htpG SO2016 0.70 -0.4 2,115,995 + htpG SO2016 0.82 +1.3 2,116,414 - -0.7 2,116,748 + SO2017 0.34 +0.4 2,116,748 + SO2017 0.34 -1.9 2,116,826 + SO2017 0.43 -1.5 2,116,834 - SO2017 0.44 +0.1 2,116,891 - SO2017 0.50 -2.8 2,116,972 - SO2017 0.60 -2.4 2,117,143 + SO2017 0.79 +0.3 2,117,143 + SO2017 0.79 -3.8 2,117,151 - SO2017 0.80 -0.1 2,117,168 + SO2017 0.82 -3.1 2,117,177 + SO2017 0.83 -0.4 2,117,299 + -2.2 2,117,300 - -0.9 2,117,307 - -0.4 2,118,092 - +0.4 2,118,153 + +1.5 2,118,201 + +0.4 2,118,289 - +0.1
Or see this region's nucleotide sequence