Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF-2 and secD-2 are separated by 13 nucleotides secD-2 and yajC are separated by 17 nucleotides yajC and tgt are separated by 26 nucleotides
SO3110: secF-2 - protein-export membrane protein SecF (NCBI ptt file), at 3,238,529 to 3,239,476
secF-2
SO3111: secD-2 - protein-export membrane protein SecD (NCBI ptt file), at 3,239,490 to 3,241,340
secD-2
SO3112: yajC - preprotein translocase, YajC subunit (NCBI ptt file), at 3,241,358 to 3,241,690
yajC
SO3113: tgt - queuine tRNA-ribosyltransferase (NCBI ptt file), at 3,241,717 to 3,242,841
tgt
Position (kb)
3239
3240
3241
3242 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3238.654 kb on + strand, within secF-2 at 3239.045 kb on + strand, within secF-2 at 3239.180 kb on + strand, within secF-2 at 3239.275 kb on - strand, within secF-2 at 3239.295 kb on - strand, within secF-2 at 3239.400 kb on - strand at 3239.407 kb on + strand at 3239.407 kb on + strand at 3239.495 kb on - strand at 3239.706 kb on - strand, within secD-2 at 3239.807 kb on - strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.829 kb on + strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.837 kb on - strand, within secD-2 at 3239.967 kb on - strand, within secD-2 at 3239.967 kb on - strand, within secD-2 at 3240.013 kb on - strand, within secD-2 at 3240.081 kb on + strand, within secD-2 at 3240.128 kb on - strand, within secD-2 at 3240.315 kb on - strand, within secD-2 at 3240.380 kb on + strand, within secD-2 at 3240.542 kb on + strand, within secD-2 at 3240.750 kb on - strand, within secD-2 at 3240.751 kb on - strand, within secD-2 at 3241.007 kb on + strand, within secD-2 at 3241.153 kb on + strand, within secD-2 at 3241.175 kb on - strand at 3241.196 kb on - strand at 3241.214 kb on - strand at 3241.342 kb on + strand at 3241.350 kb on - strand at 3241.350 kb on - strand at 3241.377 kb on - strand at 3241.651 kb on - strand, within yajC at 3241.651 kb on - strand, within yajC at 3241.913 kb on - strand, within tgt at 3242.050 kb on + strand, within tgt at 3242.071 kb on + strand, within tgt at 3242.236 kb on - strand, within tgt at 3242.286 kb on - strand, within tgt at 3242.320 kb on + strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 3,238,654 + secF-2 SO3110 0.13 -0.5 3,239,045 + secF-2 SO3110 0.54 -0.3 3,239,180 + secF-2 SO3110 0.69 -0.7 3,239,275 - secF-2 SO3110 0.79 -2.5 3,239,295 - secF-2 SO3110 0.81 -1.5 3,239,400 - -0.7 3,239,407 + -2.0 3,239,407 + -2.2 3,239,495 - -0.5 3,239,706 - secD-2 SO3111 0.12 +0.6 3,239,807 - secD-2 SO3111 0.17 -3.5 3,239,829 + secD-2 SO3111 0.18 +0.2 3,239,829 + secD-2 SO3111 0.18 -1.6 3,239,829 + secD-2 SO3111 0.18 -0.1 3,239,837 - secD-2 SO3111 0.19 -1.5 3,239,837 - secD-2 SO3111 0.19 -0.7 3,239,837 - secD-2 SO3111 0.19 +0.3 3,239,837 - secD-2 SO3111 0.19 -2.8 3,239,967 - secD-2 SO3111 0.26 -1.5 3,239,967 - secD-2 SO3111 0.26 -1.3 3,240,013 - secD-2 SO3111 0.28 -2.4 3,240,081 + secD-2 SO3111 0.32 -2.4 3,240,128 - secD-2 SO3111 0.34 -2.7 3,240,315 - secD-2 SO3111 0.45 -0.2 3,240,380 + secD-2 SO3111 0.48 -0.0 3,240,542 + secD-2 SO3111 0.57 -1.5 3,240,750 - secD-2 SO3111 0.68 -1.4 3,240,751 - secD-2 SO3111 0.68 -0.6 3,241,007 + secD-2 SO3111 0.82 +0.0 3,241,153 + secD-2 SO3111 0.90 +0.4 3,241,175 - +0.9 3,241,196 - -2.0 3,241,214 - +0.5 3,241,342 + -0.7 3,241,350 - -2.6 3,241,350 - -1.5 3,241,377 - -1.5 3,241,651 - yajC SO3112 0.88 -3.4 3,241,651 - yajC SO3112 0.88 -2.2 3,241,913 - tgt SO3113 0.17 -1.3 3,242,050 + tgt SO3113 0.30 -1.9 3,242,071 + tgt SO3113 0.31 +0.9 3,242,236 - tgt SO3113 0.46 -2.5 3,242,286 - tgt SO3113 0.51 -2.4 3,242,320 + tgt SO3113 0.54 -0.5
Or see this region's nucleotide sequence