Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25925

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25915 and BWI76_RS25920 are separated by 59 nucleotidesBWI76_RS25920 and BWI76_RS25925 are separated by 94 nucleotidesBWI76_RS25925 and BWI76_RS25930 are separated by 11 nucleotides BWI76_RS25915: BWI76_RS25915 - protein SlyX, at 5,359,169 to 5,359,387 _RS25915 BWI76_RS25920: BWI76_RS25920 - peptidylprolyl isomerase, at 5,359,447 to 5,360,034 _RS25920 BWI76_RS25925: BWI76_RS25925 - hypothetical protein, at 5,360,129 to 5,360,329 _RS25925 BWI76_RS25930: BWI76_RS25930 - glutathione-regulated potassium-efflux system protein KefB, at 5,360,341 to 5,362,146 _RS25930 Position (kb) 5360 5361Strain fitness (log2 ratio) -1 0 1at 5359.146 kb on + strandat 5359.154 kb on - strandat 5359.154 kb on - strandat 5359.154 kb on - strandat 5359.194 kb on + strand, within BWI76_RS25915at 5359.210 kb on - strand, within BWI76_RS25915at 5359.329 kb on + strand, within BWI76_RS25915at 5359.521 kb on - strand, within BWI76_RS25920at 5359.585 kb on + strand, within BWI76_RS25920at 5359.835 kb on - strand, within BWI76_RS25920at 5359.993 kb on - strandat 5360.185 kb on + strand, within BWI76_RS25925at 5360.254 kb on - strand, within BWI76_RS25925at 5360.278 kb on - strand, within BWI76_RS25925at 5360.425 kb on - strandat 5360.450 kb on - strandat 5360.628 kb on + strand, within BWI76_RS25930at 5360.703 kb on - strand, within BWI76_RS25930at 5361.199 kb on + strand, within BWI76_RS25930at 5361.259 kb on + strand, within BWI76_RS25930at 5361.267 kb on - strand, within BWI76_RS25930

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,359,146 + +0.9
5,359,154 - -1.1
5,359,154 - +0.2
5,359,154 - -0.1
5,359,194 + BWI76_RS25915 0.11 +0.1
5,359,210 - BWI76_RS25915 0.19 -0.5
5,359,329 + BWI76_RS25915 0.73 -0.4
5,359,521 - BWI76_RS25920 0.13 +0.8
5,359,585 + BWI76_RS25920 0.23 +0.5
5,359,835 - BWI76_RS25920 0.66 -0.9
5,359,993 - +0.2
5,360,185 + BWI76_RS25925 0.28 +0.4
5,360,254 - BWI76_RS25925 0.62 +1.6
5,360,278 - BWI76_RS25925 0.74 -0.9
5,360,425 - +0.3
5,360,450 - -0.1
5,360,628 + BWI76_RS25930 0.16 +0.2
5,360,703 - BWI76_RS25930 0.20 +0.3
5,361,199 + BWI76_RS25930 0.48 -0.5
5,361,259 + BWI76_RS25930 0.51 -0.1
5,361,267 - BWI76_RS25930 0.51 +0.3

Or see this region's nucleotide sequence