Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25220

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25210 and BWI76_RS25215 overlap by 1 nucleotidesBWI76_RS25215 and BWI76_RS25220 are separated by 18 nucleotidesBWI76_RS25220 and BWI76_RS25225 are separated by 4 nucleotidesBWI76_RS25225 and BWI76_RS25230 are separated by 7 nucleotides BWI76_RS25210: BWI76_RS25210 - sulfate transporter, at 5,244,110 to 5,244,400 _RS25210 BWI76_RS25215: BWI76_RS25215 - phospholipid-binding protein MlaC, at 5,244,400 to 5,245,035 _RS25215 BWI76_RS25220: BWI76_RS25220 - outer membrane lipid asymmetry maintenance protein MlaD, at 5,245,054 to 5,245,605 _RS25220 BWI76_RS25225: BWI76_RS25225 - ABC transporter permease, at 5,245,610 to 5,246,392 _RS25225 BWI76_RS25230: BWI76_RS25230 - phospholipid ABC transporter ATP-binding protein MlaF, at 5,246,400 to 5,247,212 _RS25230 Position (kb) 5245 5246Strain fitness (log2 ratio) -2 -1 0 1 2at 5244.176 kb on + strand, within BWI76_RS25210at 5244.381 kb on + strandat 5244.440 kb on + strandat 5244.624 kb on + strand, within BWI76_RS25215at 5244.666 kb on - strand, within BWI76_RS25215at 5244.677 kb on - strand, within BWI76_RS25215at 5244.737 kb on - strand, within BWI76_RS25215at 5244.890 kb on + strand, within BWI76_RS25215at 5244.956 kb on - strand, within BWI76_RS25215at 5245.147 kb on + strand, within BWI76_RS25220at 5245.147 kb on - strand, within BWI76_RS25220at 5245.224 kb on + strand, within BWI76_RS25220at 5245.268 kb on + strand, within BWI76_RS25220at 5245.326 kb on + strand, within BWI76_RS25220at 5245.369 kb on - strand, within BWI76_RS25220at 5245.572 kb on + strandat 5246.024 kb on + strand, within BWI76_RS25225at 5246.161 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.241 kb on + strand, within BWI76_RS25225at 5246.252 kb on - strand, within BWI76_RS25225at 5246.294 kb on + strand, within BWI76_RS25225at 5246.338 kb on - strandat 5246.538 kb on - strand, within BWI76_RS25230

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,244,176 + BWI76_RS25210 0.23 +0.0
5,244,381 + +0.4
5,244,440 + +0.5
5,244,624 + BWI76_RS25215 0.35 +0.7
5,244,666 - BWI76_RS25215 0.42 +0.1
5,244,677 - BWI76_RS25215 0.44 +0.0
5,244,737 - BWI76_RS25215 0.53 +0.4
5,244,890 + BWI76_RS25215 0.77 -0.1
5,244,956 - BWI76_RS25215 0.87 +1.2
5,245,147 + BWI76_RS25220 0.17 -2.7
5,245,147 - BWI76_RS25220 0.17 +0.8
5,245,224 + BWI76_RS25220 0.31 +0.9
5,245,268 + BWI76_RS25220 0.39 +0.1
5,245,326 + BWI76_RS25220 0.49 +1.7
5,245,369 - BWI76_RS25220 0.57 +2.2
5,245,572 + +0.3
5,246,024 + BWI76_RS25225 0.53 -0.2
5,246,161 + BWI76_RS25225 0.70 +1.3
5,246,241 + BWI76_RS25225 0.81 +1.7
5,246,241 + BWI76_RS25225 0.81 +1.3
5,246,252 - BWI76_RS25225 0.82 +1.7
5,246,294 + BWI76_RS25225 0.87 +0.2
5,246,338 - +1.3
5,246,538 - BWI76_RS25230 0.17 +0.3

Or see this region's nucleotide sequence