Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25065

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25060 and BWI76_RS25065 are separated by 107 nucleotidesBWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotidesBWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotidesBWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotides BWI76_RS25060: BWI76_RS25060 - lipoprotein NlpI, at 5,217,454 to 5,218,338 _RS25060 BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584 _RS25065 BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754 _RS25070 BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095 _RS25075 BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187 _RS25080 Position (kb) 5218 5219 5220 5221Strain fitness (log2 ratio) -1 0 1 2at 5217.685 kb on - strand, within BWI76_RS25060at 5217.919 kb on - strand, within BWI76_RS25060at 5218.150 kb on - strand, within BWI76_RS25060at 5218.177 kb on - strand, within BWI76_RS25060at 5218.197 kb on - strand, within BWI76_RS25060at 5218.294 kb on + strandat 5219.314 kb on + strand, within BWI76_RS25065at 5219.551 kb on + strand, within BWI76_RS25065at 5219.825 kb on + strand, within BWI76_RS25065at 5220.072 kb on - strand, within BWI76_RS25065at 5220.091 kb on + strand, within BWI76_RS25065at 5220.430 kb on + strandat 5220.472 kb on + strandat 5220.681 kb on - strand, within BWI76_RS25070at 5220.709 kb on - strandat 5221.250 kb on + strandat 5221.407 kb on - strand, within BWI76_RS25080at 5221.453 kb on + strand, within BWI76_RS25080at 5221.520 kb on + strand, within BWI76_RS25080

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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5,217,685 - BWI76_RS25060 0.26 -0.3
5,217,919 - BWI76_RS25060 0.53 -0.2
5,218,150 - BWI76_RS25060 0.79 -0.2
5,218,177 - BWI76_RS25060 0.82 -1.5
5,218,197 - BWI76_RS25060 0.84 +0.3
5,218,294 + +0.8
5,219,314 + BWI76_RS25065 0.41 +1.8
5,219,551 + BWI76_RS25065 0.52 +1.9
5,219,825 + BWI76_RS25065 0.64 +0.9
5,220,072 - BWI76_RS25065 0.76 +1.6
5,220,091 + BWI76_RS25065 0.77 +0.5
5,220,430 + +1.5
5,220,472 + -0.1
5,220,681 - BWI76_RS25070 0.65 -0.2
5,220,709 - +0.9
5,221,250 + +0.1
5,221,407 - BWI76_RS25080 0.17 +0.7
5,221,453 + BWI76_RS25080 0.22 -0.1
5,221,520 + BWI76_RS25080 0.29 +0.2

Or see this region's nucleotide sequence