Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23875

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23870 and BWI76_RS23875 are separated by 144 nucleotidesBWI76_RS23875 and BWI76_RS23880 are separated by 22 nucleotides BWI76_RS23870: BWI76_RS23870 - Glycine dehydrogenase (aminomethyl-transferring) (EC 1.4.4.2) (from data), at 4,980,471 to 4,983,344 _RS23870 BWI76_RS23875: BWI76_RS23875 - glycine cleavage system protein H, at 4,983,489 to 4,983,878 _RS23875 BWI76_RS23880: BWI76_RS23880 - Aminomethyltransferase (EC 2.1.2.10) (from data), at 4,983,901 to 4,984,995 _RS23880 Position (kb) 4983 4984Strain fitness (log2 ratio) -1 0 1at 4982.529 kb on + strand, within BWI76_RS23870at 4982.685 kb on - strand, within BWI76_RS23870at 4983.048 kb on - strand, within BWI76_RS23870at 4983.084 kb on - strandat 4983.250 kb on + strandat 4983.269 kb on + strandat 4983.334 kb on - strandat 4983.461 kb on + strandat 4983.608 kb on + strand, within BWI76_RS23875at 4983.608 kb on + strand, within BWI76_RS23875at 4983.616 kb on - strandat 4983.616 kb on - strand, within BWI76_RS23875at 4983.719 kb on + strand, within BWI76_RS23875at 4983.737 kb on + strand, within BWI76_RS23875at 4984.179 kb on + strand, within BWI76_RS23880at 4984.294 kb on + strand, within BWI76_RS23880at 4984.496 kb on + strand, within BWI76_RS23880at 4984.504 kb on - strand, within BWI76_RS23880at 4984.511 kb on + strand, within BWI76_RS23880at 4984.620 kb on - strand, within BWI76_RS23880

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,982,529 + BWI76_RS23870 0.72 +0.9
4,982,685 - BWI76_RS23870 0.77 +0.6
4,983,048 - BWI76_RS23870 0.90 +0.2
4,983,084 - +0.9
4,983,250 + +0.2
4,983,269 + +0.9
4,983,334 - +0.2
4,983,461 + +0.7
4,983,608 + BWI76_RS23875 0.31 +1.0
4,983,608 + BWI76_RS23875 0.31 +0.5
4,983,616 - +1.4
4,983,616 - BWI76_RS23875 0.33 +0.2
4,983,719 + BWI76_RS23875 0.59 +0.6
4,983,737 + BWI76_RS23875 0.64 +0.6
4,984,179 + BWI76_RS23880 0.25 +1.4
4,984,294 + BWI76_RS23880 0.36 +0.4
4,984,496 + BWI76_RS23880 0.54 +0.3
4,984,504 - BWI76_RS23880 0.55 +0.7
4,984,511 + BWI76_RS23880 0.56 +1.1
4,984,620 - BWI76_RS23880 0.66 -0.7

Or see this region's nucleotide sequence