Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22120

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22110 and BWI76_RS22115 are separated by 57 nucleotidesBWI76_RS22115 and BWI76_RS22120 are separated by 165 nucleotidesBWI76_RS22120 and BWI76_RS22125 are separated by 66 nucleotidesBWI76_RS22125 and BWI76_RS22130 overlap by 4 nucleotides BWI76_RS22110: BWI76_RS22110 - putative helix-turn-helix AraC-type transcriptional regulator, at 4,616,339 to 4,617,106 _RS22110 BWI76_RS22115: BWI76_RS22115 - S-ribosylhomocysteine lyase, at 4,617,164 to 4,617,679 _RS22115 BWI76_RS22120: BWI76_RS22120 - glutamate--cysteine ligase, at 4,617,845 to 4,619,401 _RS22120 BWI76_RS22125: BWI76_RS22125 - membrane protein, at 4,619,468 to 4,619,896 _RS22125 BWI76_RS22130: BWI76_RS22130 - fructose-1-phosphate/6-phosphogluconate phosphatase, at 4,619,893 to 4,620,459 _RS22130 Position (kb) 4617 4618 4619 4620Strain fitness (log2 ratio) -2 -1 0 1at 4616.860 kb on + strand, within BWI76_RS22110at 4616.887 kb on + strand, within BWI76_RS22110at 4616.887 kb on + strand, within BWI76_RS22110at 4616.887 kb on + strand, within BWI76_RS22110at 4616.895 kb on - strand, within BWI76_RS22110at 4616.895 kb on - strand, within BWI76_RS22110at 4616.969 kb on - strand, within BWI76_RS22110at 4617.301 kb on + strand, within BWI76_RS22115at 4617.473 kb on + strand, within BWI76_RS22115at 4617.598 kb on - strand, within BWI76_RS22115at 4617.625 kb on + strand, within BWI76_RS22115at 4617.671 kb on - strandat 4617.737 kb on + strandat 4617.755 kb on - strandat 4617.987 kb on - strandat 4618.294 kb on + strand, within BWI76_RS22120at 4618.434 kb on - strand, within BWI76_RS22120at 4618.677 kb on + strand, within BWI76_RS22120at 4618.718 kb on - strand, within BWI76_RS22120at 4618.821 kb on - strand, within BWI76_RS22120at 4618.821 kb on - strand, within BWI76_RS22120at 4618.994 kb on + strandat 4619.108 kb on - strand, within BWI76_RS22120at 4619.159 kb on + strand, within BWI76_RS22120at 4619.160 kb on - strand, within BWI76_RS22120at 4619.388 kb on - strandat 4619.594 kb on - strand, within BWI76_RS22125at 4619.772 kb on + strand, within BWI76_RS22125at 4619.780 kb on - strand, within BWI76_RS22125at 4619.847 kb on - strand, within BWI76_RS22125at 4619.881 kb on + strandat 4619.941 kb on + strandat 4620.018 kb on - strand, within BWI76_RS22130at 4620.032 kb on + strand, within BWI76_RS22130at 4620.184 kb on - strand, within BWI76_RS22130at 4620.188 kb on + strand, within BWI76_RS22130

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,616,860 + BWI76_RS22110 0.68 -0.2
4,616,887 + BWI76_RS22110 0.71 -0.4
4,616,887 + BWI76_RS22110 0.71 +0.3
4,616,887 + BWI76_RS22110 0.71 -0.2
4,616,895 - BWI76_RS22110 0.72 -0.2
4,616,895 - BWI76_RS22110 0.72 +0.7
4,616,969 - BWI76_RS22110 0.82 -0.4
4,617,301 + BWI76_RS22115 0.27 +0.4
4,617,473 + BWI76_RS22115 0.60 +0.0
4,617,598 - BWI76_RS22115 0.84 +0.2
4,617,625 + BWI76_RS22115 0.89 -0.8
4,617,671 - -0.4
4,617,737 + -0.5
4,617,755 - -0.7
4,617,987 - +0.0
4,618,294 + BWI76_RS22120 0.29 +0.7
4,618,434 - BWI76_RS22120 0.38 +0.6
4,618,677 + BWI76_RS22120 0.53 +1.6
4,618,718 - BWI76_RS22120 0.56 +0.6
4,618,821 - BWI76_RS22120 0.63 +0.5
4,618,821 - BWI76_RS22120 0.63 -0.2
4,618,994 + -0.2
4,619,108 - BWI76_RS22120 0.81 +0.3
4,619,159 + BWI76_RS22120 0.84 +0.7
4,619,160 - BWI76_RS22120 0.84 +0.4
4,619,388 - -1.0
4,619,594 - BWI76_RS22125 0.29 -1.7
4,619,772 + BWI76_RS22125 0.71 +0.1
4,619,780 - BWI76_RS22125 0.73 -0.1
4,619,847 - BWI76_RS22125 0.88 -2.3
4,619,881 + -1.0
4,619,941 + +0.9
4,620,018 - BWI76_RS22130 0.22 +0.2
4,620,032 + BWI76_RS22130 0.25 +0.8
4,620,184 - BWI76_RS22130 0.51 +0.5
4,620,188 + BWI76_RS22130 0.52 +1.4

Or see this region's nucleotide sequence