Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21385

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21380 and BWI76_RS21385 are separated by 55 nucleotidesBWI76_RS21385 and BWI76_RS21390 are separated by 203 nucleotidesBWI76_RS21390 and BWI76_RS21395 are separated by 69 nucleotides BWI76_RS21380: BWI76_RS21380 - uracil-DNA glycosylase, at 4,475,640 to 4,476,329 _RS21380 BWI76_RS21385: BWI76_RS21385 - tRNA/rRNA methyltransferase, at 4,476,385 to 4,477,458 _RS21385 BWI76_RS21390: BWI76_RS21390 - thiol disulfide reductase thioredoxin, at 4,477,662 to 4,478,087 _RS21390 BWI76_RS21395: BWI76_RS21395 - DTW domain-containing protein, at 4,478,157 to 4,478,855 _RS21395 Position (kb) 4476 4477 4478Strain fitness (log2 ratio) -2 -1 0 1at 4475.409 kb on - strandat 4475.499 kb on + strandat 4475.796 kb on - strand, within BWI76_RS21380at 4475.819 kb on - strand, within BWI76_RS21380at 4476.449 kb on - strandat 4476.457 kb on - strandat 4476.489 kb on - strandat 4476.489 kb on - strandat 4476.566 kb on + strand, within BWI76_RS21385at 4476.574 kb on - strand, within BWI76_RS21385at 4476.862 kb on - strand, within BWI76_RS21385at 4477.005 kb on + strand, within BWI76_RS21385at 4477.019 kb on - strand, within BWI76_RS21385at 4477.205 kb on - strand, within BWI76_RS21385at 4477.231 kb on - strand, within BWI76_RS21385at 4477.368 kb on + strandat 4477.368 kb on + strandat 4477.376 kb on - strandat 4477.415 kb on + strandat 4477.566 kb on - strandat 4477.655 kb on - strandat 4477.689 kb on + strandat 4477.899 kb on + strand, within BWI76_RS21390at 4477.936 kb on - strand, within BWI76_RS21390at 4478.299 kb on - strand, within BWI76_RS21395

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,475,409 - -0.1
4,475,499 + +0.1
4,475,796 - BWI76_RS21380 0.23 -0.1
4,475,819 - BWI76_RS21380 0.26 +0.2
4,476,449 - +0.6
4,476,457 - -0.2
4,476,489 - -0.0
4,476,489 - +0.8
4,476,566 + BWI76_RS21385 0.17 -1.3
4,476,574 - BWI76_RS21385 0.18 +0.0
4,476,862 - BWI76_RS21385 0.44 +0.3
4,477,005 + BWI76_RS21385 0.58 +1.1
4,477,019 - BWI76_RS21385 0.59 +1.0
4,477,205 - BWI76_RS21385 0.76 +0.7
4,477,231 - BWI76_RS21385 0.79 +0.4
4,477,368 + -1.8
4,477,368 + -1.0
4,477,376 - -0.3
4,477,415 + +0.4
4,477,566 - +0.1
4,477,655 - +0.8
4,477,689 + -0.0
4,477,899 + BWI76_RS21390 0.56 -0.1
4,477,936 - BWI76_RS21390 0.64 +0.1
4,478,299 - BWI76_RS21395 0.20 -0.2

Or see this region's nucleotide sequence