Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21375

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21370 and BWI76_RS21375 are separated by 44 nucleotidesBWI76_RS21375 and BWI76_RS21380 are separated by 311 nucleotides BWI76_RS21370: BWI76_RS21370 - ATP-dependent RNA helicase SrmB, at 4,473,569 to 4,474,900 _RS21370 BWI76_RS21375: BWI76_RS21375 - autonomous glycyl radical cofactor GrcA, at 4,474,945 to 4,475,328 _RS21375 BWI76_RS21380: BWI76_RS21380 - uracil-DNA glycosylase, at 4,475,640 to 4,476,329 _RS21380 Position (kb) 4474 4475 4476Strain fitness (log2 ratio) -2 -1 0 1at 4475.120 kb on - strand, within BWI76_RS21375at 4475.276 kb on - strand, within BWI76_RS21375at 4475.409 kb on - strandat 4475.499 kb on + strandat 4475.796 kb on - strand, within BWI76_RS21380at 4475.819 kb on - strand, within BWI76_RS21380

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,475,120 - BWI76_RS21375 0.46 -1.8
4,475,276 - BWI76_RS21375 0.86 +1.0
4,475,409 - -0.1
4,475,499 + +0.1
4,475,796 - BWI76_RS21380 0.23 -0.1
4,475,819 - BWI76_RS21380 0.26 +0.2

Or see this region's nucleotide sequence