Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20750

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20745 and BWI76_RS20750 are separated by 48 nucleotidesBWI76_RS20750 and BWI76_RS20755 are separated by 10 nucleotidesBWI76_RS20755 and BWI76_RS20760 are separated by 9 nucleotides BWI76_RS20745: BWI76_RS20745 - AraC family transcriptional regulator, at 4,336,511 to 4,337,563 _RS20745 BWI76_RS20750: BWI76_RS20750 - carboxysome structural protein EutK, at 4,337,612 to 4,338,103 _RS20750 BWI76_RS20755: BWI76_RS20755 - microcompartment protein EutL, at 4,338,114 to 4,338,773 _RS20755 BWI76_RS20760: BWI76_RS20760 - ethanolamine ammonia-lyase small subunit, at 4,338,783 to 4,339,676 _RS20760 Position (kb) 4337 4338 4339Strain fitness (log2 ratio) -1 0 1at 4336.633 kb on - strand, within BWI76_RS20745at 4336.656 kb on - strand, within BWI76_RS20745at 4336.663 kb on + strand, within BWI76_RS20745at 4336.788 kb on - strand, within BWI76_RS20745at 4336.818 kb on + strand, within BWI76_RS20745at 4336.935 kb on - strand, within BWI76_RS20745at 4337.033 kb on - strand, within BWI76_RS20745at 4337.147 kb on - strand, within BWI76_RS20745at 4337.148 kb on - strand, within BWI76_RS20745at 4337.296 kb on + strand, within BWI76_RS20745at 4337.400 kb on + strand, within BWI76_RS20745at 4337.439 kb on - strand, within BWI76_RS20745at 4337.969 kb on - strand, within BWI76_RS20750at 4338.257 kb on - strand, within BWI76_RS20755at 4338.576 kb on + strand, within BWI76_RS20755at 4338.587 kb on - strand, within BWI76_RS20755at 4338.667 kb on - strand, within BWI76_RS20755at 4338.714 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,336,633 - BWI76_RS20745 0.12 +0.6
4,336,656 - BWI76_RS20745 0.14 -0.2
4,336,663 + BWI76_RS20745 0.14 -0.2
4,336,788 - BWI76_RS20745 0.26 -0.1
4,336,818 + BWI76_RS20745 0.29 +0.3
4,336,935 - BWI76_RS20745 0.40 +0.1
4,337,033 - BWI76_RS20745 0.50 -0.0
4,337,147 - BWI76_RS20745 0.60 +0.1
4,337,148 - BWI76_RS20745 0.60 +0.0
4,337,296 + BWI76_RS20745 0.75 -0.1
4,337,400 + BWI76_RS20745 0.84 -1.3
4,337,439 - BWI76_RS20745 0.88 -0.4
4,337,969 - BWI76_RS20750 0.73 +0.7
4,338,257 - BWI76_RS20755 0.22 +0.3
4,338,576 + BWI76_RS20755 0.70 +0.0
4,338,587 - BWI76_RS20755 0.72 +0.2
4,338,667 - BWI76_RS20755 0.84 +0.7
4,338,714 + -1.7

Or see this region's nucleotide sequence