Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20720

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20710 and BWI76_RS20715 are separated by 96 nucleotidesBWI76_RS20715 and BWI76_RS20720 are separated by 63 nucleotidesBWI76_RS20720 and BWI76_RS20725 overlap by 14 nucleotidesBWI76_RS20725 and BWI76_RS20730 are separated by 21 nucleotidesBWI76_RS20730 and BWI76_RS20735 overlap by 8 nucleotides BWI76_RS20710: BWI76_RS20710 - dyp-type peroxidase family protein, at 4,331,895 to 4,332,794 _RS20710 BWI76_RS20715: BWI76_RS20715 - RpoE-regulated lipoprotein, at 4,332,891 to 4,333,466 _RS20715 BWI76_RS20720: BWI76_RS20720 - hypothetical protein, at 4,333,530 to 4,333,979 _RS20720 BWI76_RS20725: BWI76_RS20725 - GNAT family acetyltransferase, at 4,333,966 to 4,334,391 _RS20725 BWI76_RS20730: BWI76_RS20730 - hypothetical protein, at 4,334,413 to 4,334,610 _RS20730 BWI76_RS20735: BWI76_RS20735 - N-acetylmuramoyl-L-alanine amidase, at 4,334,603 to 4,335,478 _RS20735 Position (kb) 4333 4334Strain fitness (log2 ratio) -1 0 1at 4333.346 kb on + strand, within BWI76_RS20715at 4333.453 kb on - strandat 4333.570 kb on - strandat 4333.570 kb on - strandat 4333.842 kb on - strand, within BWI76_RS20720at 4333.901 kb on - strand, within BWI76_RS20720at 4334.034 kb on - strand, within BWI76_RS20725at 4334.262 kb on + strand, within BWI76_RS20725at 4334.325 kb on - strand, within BWI76_RS20725at 4334.337 kb on + strand, within BWI76_RS20725at 4334.345 kb on + strand, within BWI76_RS20725at 4334.394 kb on - strandat 4334.403 kb on + strandat 4334.403 kb on - strandat 4334.420 kb on - strandat 4334.572 kb on - strand, within BWI76_RS20730at 4334.904 kb on + strand, within BWI76_RS20735at 4334.945 kb on - strand, within BWI76_RS20735

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,333,346 + BWI76_RS20715 0.79 -0.0
4,333,453 - +0.1
4,333,570 - -0.3
4,333,570 - -0.2
4,333,842 - BWI76_RS20720 0.69 +0.5
4,333,901 - BWI76_RS20720 0.82 +1.3
4,334,034 - BWI76_RS20725 0.16 -0.2
4,334,262 + BWI76_RS20725 0.69 -0.0
4,334,325 - BWI76_RS20725 0.84 +0.4
4,334,337 + BWI76_RS20725 0.87 +0.8
4,334,345 + BWI76_RS20725 0.89 -0.2
4,334,394 - +0.0
4,334,403 + +0.1
4,334,403 - +0.1
4,334,420 - +0.3
4,334,572 - BWI76_RS20730 0.80 -0.2
4,334,904 + BWI76_RS20735 0.34 -0.8
4,334,945 - BWI76_RS20735 0.39 +0.4

Or see this region's nucleotide sequence