Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20640 and BWI76_RS20645 are separated by 381 nucleotides BWI76_RS20645 and BWI76_RS20650 are separated by 44 nucleotides BWI76_RS20650 and BWI76_RS20655 are separated by 42 nucleotides BWI76_RS20655 and BWI76_RS20660 are separated by 42 nucleotides BWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotides
BWI76_RS20640: BWI76_RS20640 - cysteine synthase A, at 4,319,208 to 4,320,179
_RS20640
BWI76_RS20645: BWI76_RS20645 - phosphocarrier protein HPr, at 4,320,561 to 4,320,818
_RS20645
BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590
_RS20650
BWI76_RS20655: BWI76_RS20655 - glucose-specific phosphotransferase enzyme IIA component, at 4,322,633 to 4,323,142
_RS20655
BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424
_RS20660
BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287
_RS20665
Position (kb) 4320
4321
4322
4323 Strain fitness (log2 ratio) -1
0
1
2
3 at 4319.865 kb on - strand, within BWI76_RS20640 at 4320.263 kb on - strand at 4320.449 kb on + strand at 4320.786 kb on - strand, within BWI76_RS20645 at 4321.146 kb on - strand, within BWI76_RS20650 at 4321.357 kb on + strand, within BWI76_RS20650 at 4321.684 kb on + strand, within BWI76_RS20650 at 4322.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 4,319,865 - BWI76_RS20640 0.68 +0.8 4,320,263 - +0.1 4,320,449 + +0.5 4,320,786 - BWI76_RS20645 0.87 +1.2 4,321,146 - BWI76_RS20650 0.16 +3.6 4,321,357 + BWI76_RS20650 0.29 +2.4 4,321,684 + BWI76_RS20650 0.48 +1.4 4,322,527 + +0.7
Or see this region's nucleotide sequence