Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20300 and BWI76_RS20305 are separated by 217 nucleotides BWI76_RS20305 and BWI76_RS20310 are separated by 3 nucleotides BWI76_RS20310 and BWI76_RS20315 are separated by 56 nucleotides BWI76_RS20315 and BWI76_RS20320 are separated by 41 nucleotides
BWI76_RS20300: BWI76_RS20300 - epimerase, at 4,252,241 to 4,253,134
_RS20300
BWI76_RS20305: BWI76_RS20305 - hypothetical protein, at 4,253,352 to 4,253,606
_RS20305
BWI76_RS20310: BWI76_RS20310 - hypothetical protein, at 4,253,610 to 4,254,176
_RS20310
BWI76_RS20315: BWI76_RS20315 - N-acetyltransferase, at 4,254,233 to 4,254,730
_RS20315
BWI76_RS20320: BWI76_RS20320 - histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP, at 4,254,772 to 4,255,545
_RS20320
Position (kb)
4253
4254
4255 Strain fitness (log2 ratio)
-1
0
1 at 4253.258 kb on + strand at 4253.270 kb on + strand at 4253.270 kb on + strand at 4253.270 kb on + strand at 4253.333 kb on + strand at 4253.641 kb on - strand at 4254.107 kb on + strand, within BWI76_RS20310 at 4254.107 kb on - strand, within BWI76_RS20310 at 4254.115 kb on - strand, within BWI76_RS20310 at 4254.122 kb on + strand at 4254.354 kb on + strand, within BWI76_RS20315 at 4254.505 kb on - strand, within BWI76_RS20315 at 4254.561 kb on + strand, within BWI76_RS20315 at 4254.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 4,253,258 + +0.2 4,253,270 + +0.7 4,253,270 + -0.5 4,253,270 + -0.2 4,253,333 + -0.4 4,253,641 - -0.6 4,254,107 + BWI76_RS20310 0.88 +0.7 4,254,107 - BWI76_RS20310 0.88 +1.0 4,254,115 - BWI76_RS20310 0.89 -0.2 4,254,122 + -0.2 4,254,354 + BWI76_RS20315 0.24 +0.2 4,254,505 - BWI76_RS20315 0.55 -0.9 4,254,561 + BWI76_RS20315 0.66 +0.0 4,254,777 - +0.0
Or see this region's nucleotide sequence