Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20275 and BWI76_RS20280 are separated by 58 nucleotides BWI76_RS20280 and BWI76_RS20285 are separated by 56 nucleotides BWI76_RS20285 and BWI76_RS20290 are separated by 208 nucleotides BWI76_RS20290 and BWI76_RS20295 are separated by 65 nucleotides
BWI76_RS20275: BWI76_RS20275 - NUDIX hydrolase, at 4,249,086 to 4,249,640
_RS20275
BWI76_RS20280: BWI76_RS20280 - phosphodiesterase, at 4,249,699 to 4,250,250
_RS20280
BWI76_RS20285: BWI76_RS20285 - glutathione S-transferase, at 4,250,307 to 4,250,951
_RS20285
BWI76_RS20290: BWI76_RS20290 - thiol:disulfide oxidoreductase, at 4,251,160 to 4,251,789
_RS20290
BWI76_RS20295: BWI76_RS20295 - dihydroneopterin triphosphate 2'-epimerase, at 4,251,855 to 4,252,217
_RS20295
Position (kb) 4250
4251 Strain fitness (log2 ratio) -1
0
1 at 4249.402 kb on - strand, within BWI76_RS20275 at 4249.673 kb on - strand at 4249.776 kb on + strand, within BWI76_RS20280 at 4249.885 kb on - strand, within BWI76_RS20280 at 4249.922 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.226 kb on - strand at 4250.372 kb on - strand, within BWI76_RS20285 at 4250.639 kb on - strand, within BWI76_RS20285 at 4250.849 kb on + strand, within BWI76_RS20285 at 4250.946 kb on + strand at 4251.047 kb on + strand at 4251.055 kb on - strand at 4251.062 kb on - strand at 4251.082 kb on + strand at 4251.261 kb on - strand, within BWI76_RS20290 at 4251.364 kb on - strand, within BWI76_RS20290 at 4251.451 kb on - strand, within BWI76_RS20290 at 4251.611 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.715 kb on + strand, within BWI76_RS20290 at 4251.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 4,249,402 - BWI76_RS20275 0.57 -0.7 4,249,673 - -0.4 4,249,776 + BWI76_RS20280 0.14 +0.9 4,249,885 - BWI76_RS20280 0.34 -1.2 4,249,922 + BWI76_RS20280 0.40 +0.7 4,250,187 + BWI76_RS20280 0.88 -0.8 4,250,187 + BWI76_RS20280 0.88 +0.6 4,250,226 - +0.0 4,250,372 - BWI76_RS20285 0.10 +0.1 4,250,639 - BWI76_RS20285 0.51 +0.1 4,250,849 + BWI76_RS20285 0.84 +1.2 4,250,946 + +0.7 4,251,047 + +0.8 4,251,055 - +0.3 4,251,062 - +0.7 4,251,082 + +0.0 4,251,261 - BWI76_RS20290 0.16 +0.2 4,251,364 - BWI76_RS20290 0.32 -0.4 4,251,451 - BWI76_RS20290 0.46 +0.1 4,251,611 + BWI76_RS20290 0.72 -0.5 4,251,715 + BWI76_RS20290 0.88 +0.9 4,251,715 + BWI76_RS20290 0.88 +0.3 4,251,847 + +0.6
Or see this region's nucleotide sequence