Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19495

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19480 and BWI76_RS19485 are separated by 159 nucleotidesBWI76_RS19485 and BWI76_RS19490 overlap by 26 nucleotidesBWI76_RS19490 and BWI76_RS19495 overlap by 17 nucleotidesBWI76_RS19495 and BWI76_RS19500 overlap by 8 nucleotides BWI76_RS19480: BWI76_RS19480 - transcriptional regulator, at 4,071,356 to 4,072,114 _RS19480 BWI76_RS19485: BWI76_RS19485 - membrane protein, at 4,072,274 to 4,072,387 _RS19485 BWI76_RS19490: BWI76_RS19490 - binding-protein-dependent transport system inner membrane protein, at 4,072,362 to 4,073,099 _RS19490 BWI76_RS19495: BWI76_RS19495 - ABC transporter, quaternary amine uptake transporter (QAT) family, ATP-binding protein, at 4,073,083 to 4,074,030 _RS19495 BWI76_RS19500: BWI76_RS19500 - osmoprotectant uptake system permease, at 4,074,023 to 4,075,180 _RS19500 Position (kb) 4073 4074 4075Strain fitness (log2 ratio) -1 0 1 2at 4072.173 kb on - strandat 4072.179 kb on + strandat 4072.249 kb on - strandat 4072.623 kb on - strand, within BWI76_RS19490at 4072.733 kb on + strand, within BWI76_RS19490at 4072.941 kb on - strandat 4073.016 kb on - strand, within BWI76_RS19490at 4073.756 kb on + strand, within BWI76_RS19495at 4074.047 kb on - strandat 4074.077 kb on + strandat 4074.190 kb on - strand, within BWI76_RS19500at 4074.236 kb on + strand, within BWI76_RS19500at 4074.257 kb on + strand, within BWI76_RS19500at 4074.351 kb on - strand, within BWI76_RS19500at 4074.369 kb on - strand, within BWI76_RS19500at 4074.406 kb on + strandat 4074.869 kb on + strand, within BWI76_RS19500at 4074.892 kb on - strand, within BWI76_RS19500at 4074.897 kb on - strand, within BWI76_RS19500

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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4,072,173 - +0.1
4,072,179 + +1.2
4,072,249 - -0.4
4,072,623 - BWI76_RS19490 0.35 +0.2
4,072,733 + BWI76_RS19490 0.50 +0.5
4,072,941 - +0.2
4,073,016 - BWI76_RS19490 0.89 -0.2
4,073,756 + BWI76_RS19495 0.71 +0.6
4,074,047 - +0.1
4,074,077 + -0.6
4,074,190 - BWI76_RS19500 0.14 +1.8
4,074,236 + BWI76_RS19500 0.18 +0.1
4,074,257 + BWI76_RS19500 0.20 -0.2
4,074,351 - BWI76_RS19500 0.28 +0.9
4,074,369 - BWI76_RS19500 0.30 -0.2
4,074,406 + +0.8
4,074,869 + BWI76_RS19500 0.73 +0.2
4,074,892 - BWI76_RS19500 0.75 -0.3
4,074,897 - BWI76_RS19500 0.75 +0.4

Or see this region's nucleotide sequence