Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19145

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19140 and BWI76_RS19145 are separated by 280 nucleotidesBWI76_RS19145 and BWI76_RS19150 overlap by 4 nucleotidesBWI76_RS19150 and BWI76_RS19155 are separated by 12 nucleotides BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723 _RS19140 BWI76_RS19145: BWI76_RS19145 - formate dehydrogenase cytochrome b556 subunit, at 3,981,004 to 3,981,633 _RS19145 BWI76_RS19150: BWI76_RS19150 - formate dehydrogenase subunit beta, at 3,981,630 to 3,982,532 _RS19150 BWI76_RS19155: BWI76_RS19155 - formate dehydrogenase-N subunit alpha, at 3,982,545 to 3,985,595 _RS19155 Position (kb) 3981 3982Strain fitness (log2 ratio) -1 0 1at 3980.306 kb on + strand, within BWI76_RS19140at 3980.319 kb on + strand, within BWI76_RS19140at 3980.319 kb on + strand, within BWI76_RS19140at 3980.327 kb on - strand, within BWI76_RS19140at 3980.484 kb on - strand, within BWI76_RS19140at 3980.662 kb on + strandat 3981.070 kb on - strand, within BWI76_RS19145

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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3,980,306 + BWI76_RS19140 0.68 -0.9
3,980,319 + BWI76_RS19140 0.69 +1.7
3,980,319 + BWI76_RS19140 0.69 -1.3
3,980,327 - BWI76_RS19140 0.69 +1.0
3,980,484 - BWI76_RS19140 0.81 +0.6
3,980,662 + -1.4
3,981,070 - BWI76_RS19145 0.10 +0.8

Or see this region's nucleotide sequence