Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16355

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16345 and BWI76_RS16350 are separated by 79 nucleotidesBWI76_RS16350 and BWI76_RS16355 are separated by 77 nucleotidesBWI76_RS16355 and BWI76_RS16360 overlap by 1 nucleotidesBWI76_RS16360 and BWI76_RS16365 overlap by 8 nucleotides BWI76_RS16345: BWI76_RS16345 - nucleoid-associated protein, at 3,392,789 to 3,393,004 _RS16345 BWI76_RS16350: BWI76_RS16350 - membrane protein, at 3,393,084 to 3,393,524 _RS16350 BWI76_RS16355: BWI76_RS16355 - electron transport complex subunit A, at 3,393,602 to 3,394,183 _RS16355 BWI76_RS16360: BWI76_RS16360 - electron transport complex subunit RsxB, at 3,394,183 to 3,394,761 _RS16360 BWI76_RS16365: BWI76_RS16365 - electron transport complex subunit RsxC, at 3,394,754 to 3,396,814 _RS16365 Position (kb) 3393 3394 3395Strain fitness (log2 ratio) -2 -1 0 1 2at 3392.617 kb on - strandat 3392.718 kb on + strandat 3392.723 kb on + strandat 3392.728 kb on + strandat 3392.728 kb on + strandat 3392.731 kb on - strandat 3392.736 kb on + strandat 3392.736 kb on - strandat 3392.743 kb on + strandat 3392.863 kb on + strand, within BWI76_RS16345at 3392.871 kb on - strand, within BWI76_RS16345at 3392.871 kb on - strand, within BWI76_RS16345at 3392.873 kb on + strand, within BWI76_RS16345at 3392.883 kb on + strand, within BWI76_RS16345at 3392.913 kb on + strand, within BWI76_RS16345at 3392.931 kb on - strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.974 kb on + strand, within BWI76_RS16345at 3392.980 kb on - strand, within BWI76_RS16345at 3392.982 kb on - strand, within BWI76_RS16345at 3392.982 kb on - strand, within BWI76_RS16345at 3393.006 kb on - strandat 3393.029 kb on + strandat 3393.029 kb on + strandat 3393.037 kb on - strandat 3393.145 kb on + strand, within BWI76_RS16350at 3393.155 kb on + strand, within BWI76_RS16350at 3393.158 kb on - strand, within BWI76_RS16350at 3393.209 kb on + strand, within BWI76_RS16350at 3393.219 kb on - strand, within BWI76_RS16350at 3393.243 kb on + strand, within BWI76_RS16350at 3393.254 kb on + strand, within BWI76_RS16350at 3393.293 kb on - strand, within BWI76_RS16350at 3393.295 kb on - strand, within BWI76_RS16350at 3393.298 kb on - strand, within BWI76_RS16350at 3393.383 kb on - strand, within BWI76_RS16350at 3393.533 kb on + strandat 3393.551 kb on - strandat 3393.672 kb on - strand, within BWI76_RS16355at 3393.681 kb on + strand, within BWI76_RS16355at 3393.713 kb on + strand, within BWI76_RS16355at 3393.970 kb on + strand, within BWI76_RS16355at 3394.192 kb on + strandat 3394.369 kb on - strand, within BWI76_RS16360at 3394.890 kb on + strandat 3395.046 kb on - strand, within BWI76_RS16365

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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3,392,617 - -0.2
3,392,718 + +0.4
3,392,723 + +0.1
3,392,728 + -0.5
3,392,728 + -0.1
3,392,731 - +0.2
3,392,736 + -0.5
3,392,736 - +0.4
3,392,743 + -1.1
3,392,863 + BWI76_RS16345 0.34 -0.5
3,392,871 - BWI76_RS16345 0.38 -0.9
3,392,871 - BWI76_RS16345 0.38 -0.2
3,392,873 + BWI76_RS16345 0.39 -0.3
3,392,883 + BWI76_RS16345 0.44 +0.4
3,392,913 + BWI76_RS16345 0.57 -1.1
3,392,931 - BWI76_RS16345 0.66 -0.6
3,392,974 + BWI76_RS16345 0.86 -1.1
3,392,974 + BWI76_RS16345 0.86 -0.1
3,392,974 + BWI76_RS16345 0.86 -2.1
3,392,974 + BWI76_RS16345 0.86 -0.3
3,392,974 + BWI76_RS16345 0.86 -0.5
3,392,974 + BWI76_RS16345 0.86 +0.2
3,392,974 + BWI76_RS16345 0.86 -0.9
3,392,974 + BWI76_RS16345 0.86 +0.1
3,392,974 + BWI76_RS16345 0.86 +0.3
3,392,974 + BWI76_RS16345 0.86 +0.7
3,392,974 + BWI76_RS16345 0.86 +0.0
3,392,980 - BWI76_RS16345 0.88 -0.5
3,392,982 - BWI76_RS16345 0.89 -0.1
3,392,982 - BWI76_RS16345 0.89 -0.2
3,393,006 - -0.7
3,393,029 + -0.2
3,393,029 + -0.5
3,393,037 - -0.8
3,393,145 + BWI76_RS16350 0.14 -0.1
3,393,155 + BWI76_RS16350 0.16 -0.3
3,393,158 - BWI76_RS16350 0.17 +1.7
3,393,209 + BWI76_RS16350 0.28 -0.2
3,393,219 - BWI76_RS16350 0.31 +0.2
3,393,243 + BWI76_RS16350 0.36 -0.3
3,393,254 + BWI76_RS16350 0.39 -0.4
3,393,293 - BWI76_RS16350 0.47 -0.4
3,393,295 - BWI76_RS16350 0.48 +0.4
3,393,298 - BWI76_RS16350 0.49 -0.9
3,393,383 - BWI76_RS16350 0.68 +0.3
3,393,533 + -0.5
3,393,551 - -0.1
3,393,672 - BWI76_RS16355 0.12 +0.4
3,393,681 + BWI76_RS16355 0.14 +2.1
3,393,713 + BWI76_RS16355 0.19 +0.7
3,393,970 + BWI76_RS16355 0.63 +1.0
3,394,192 + -0.7
3,394,369 - BWI76_RS16360 0.32 -0.8
3,394,890 + -1.4
3,395,046 - BWI76_RS16365 0.14 +0.3

Or see this region's nucleotide sequence