Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15205

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15200 and BWI76_RS15205 are separated by 204 nucleotidesBWI76_RS15205 and BWI76_RS15210 are separated by 105 nucleotidesBWI76_RS15210 and BWI76_RS15215 overlap by 47 nucleotides BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938 _RS15200 BWI76_RS15205: BWI76_RS15205 - succinate-semialdehyde dehydrogenase, at 3,187,143 to 3,188,528 _RS15205 BWI76_RS15210: BWI76_RS15210 - LysR family transcriptional regulator, at 3,188,634 to 3,189,512 _RS15210 BWI76_RS15215: BWI76_RS15215 - N-acetyltransferase, at 3,189,466 to 3,189,948 _RS15215 Position (kb) 3187 3188 3189Strain fitness (log2 ratio) -1 0 1 2at 3186.326 kb on + strand, within BWI76_RS15200at 3186.539 kb on - strand, within BWI76_RS15200at 3187.198 kb on - strandat 3187.473 kb on + strand, within BWI76_RS15205at 3187.555 kb on - strand, within BWI76_RS15205at 3188.031 kb on + strand, within BWI76_RS15205at 3188.691 kb on - strandat 3188.970 kb on - strand, within BWI76_RS15210at 3189.178 kb on + strand, within BWI76_RS15210at 3189.322 kb on + strand, within BWI76_RS15210at 3189.351 kb on - strand, within BWI76_RS15210

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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3,186,326 + BWI76_RS15200 0.34 +0.1
3,186,539 - BWI76_RS15200 0.57 -0.0
3,187,198 - -0.4
3,187,473 + BWI76_RS15205 0.24 +1.9
3,187,555 - BWI76_RS15205 0.30 +0.8
3,188,031 + BWI76_RS15205 0.64 +0.2
3,188,691 - +0.6
3,188,970 - BWI76_RS15210 0.38 +0.7
3,189,178 + BWI76_RS15210 0.62 +0.7
3,189,322 + BWI76_RS15210 0.78 -1.2
3,189,351 - BWI76_RS15210 0.82 -0.1

Or see this region's nucleotide sequence