Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13145 and BWI76_RS13150 are separated by 9 nucleotides BWI76_RS13150 and BWI76_RS13155 are separated by 11 nucleotides BWI76_RS13155 and BWI76_RS13160 are separated by 35 nucleotides BWI76_RS13160 and BWI76_RS13165 are separated by 204 nucleotides
BWI76_RS13145: BWI76_RS13145 - phenylacetic acid degradation operon negative regulatory protein PaaX, at 2,741,574 to 2,742,509
_RS13145
BWI76_RS13150: BWI76_RS13150 - carnitine operon protein CaiE, at 2,742,519 to 2,743,115
_RS13150
BWI76_RS13155: BWI76_RS13155 - hypothetical protein, at 2,743,127 to 2,743,534
_RS13155
BWI76_RS13160: BWI76_RS13160 - azoreductase, at 2,743,570 to 2,744,175
_RS13160
BWI76_RS13165: BWI76_RS13165 - ATP-dependent RNA helicase HrpA, at 2,744,380 to 2,748,282
_RS13165
Position (kb) 2743
2744 Strain fitness (log2 ratio) -1
0
1
2 at 2742.728 kb on + strand, within BWI76_RS13150 at 2743.184 kb on - strand, within BWI76_RS13155 at 2743.203 kb on - strand, within BWI76_RS13155 at 2743.294 kb on + strand, within BWI76_RS13155 at 2743.302 kb on - strand, within BWI76_RS13155 at 2743.707 kb on + strand, within BWI76_RS13160 at 2744.165 kb on + strand at 2744.220 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,742,728 + BWI76_RS13150 0.35 -0.1 2,743,184 - BWI76_RS13155 0.14 +0.6 2,743,203 - BWI76_RS13155 0.19 +0.8 2,743,294 + BWI76_RS13155 0.41 +0.1 2,743,302 - BWI76_RS13155 0.43 +2.5 2,743,707 + BWI76_RS13160 0.23 -0.2 2,744,165 + -0.5 2,744,220 - +2.2
Or see this region's nucleotide sequence