Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12820

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12815 and BWI76_RS12820 are separated by 235 nucleotidesBWI76_RS12820 and BWI76_RS12825 are separated by 131 nucleotidesBWI76_RS12825 and BWI76_RS12830 are separated by 4 nucleotides BWI76_RS12815: BWI76_RS12815 - transcriptional regulator FeaR, at 2,670,113 to 2,671,021 _RS12815 BWI76_RS12820: BWI76_RS12820 - NAD-dependent phenylacetaldehyde dehydrogenase, at 2,671,257 to 2,672,756 _RS12820 BWI76_RS12825: BWI76_RS12825 - hypothetical protein, at 2,672,888 to 2,673,619 _RS12825 BWI76_RS12830: BWI76_RS12830 - flagellar motor switch protein FliY, at 2,673,624 to 2,674,454 _RS12830 Position (kb) 2671 2672 2673Strain fitness (log2 ratio) -1 0 1at 2670.529 kb on + strand, within BWI76_RS12815at 2670.614 kb on + strand, within BWI76_RS12815at 2671.231 kb on - strandat 2671.242 kb on + strandat 2671.441 kb on + strand, within BWI76_RS12820at 2671.485 kb on + strand, within BWI76_RS12820at 2671.493 kb on - strand, within BWI76_RS12820at 2671.581 kb on - strand, within BWI76_RS12820at 2671.609 kb on + strandat 2671.970 kb on - strand, within BWI76_RS12820at 2672.084 kb on + strand, within BWI76_RS12820at 2672.296 kb on - strand, within BWI76_RS12820at 2672.415 kb on - strand, within BWI76_RS12820at 2672.527 kb on - strand, within BWI76_RS12820at 2672.640 kb on - strandat 2672.640 kb on - strandat 2672.899 kb on - strandat 2673.302 kb on + strand, within BWI76_RS12825

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,670,529 + BWI76_RS12815 0.46 +1.4
2,670,614 + BWI76_RS12815 0.55 +0.2
2,671,231 - -0.1
2,671,242 + +0.4
2,671,441 + BWI76_RS12820 0.12 -1.2
2,671,485 + BWI76_RS12820 0.15 +0.4
2,671,493 - BWI76_RS12820 0.16 -0.6
2,671,581 - BWI76_RS12820 0.22 +0.8
2,671,609 + -1.6
2,671,970 - BWI76_RS12820 0.48 +0.5
2,672,084 + BWI76_RS12820 0.55 +0.9
2,672,296 - BWI76_RS12820 0.69 +1.0
2,672,415 - BWI76_RS12820 0.77 +1.0
2,672,527 - BWI76_RS12820 0.85 -0.4
2,672,640 - +1.6
2,672,640 - +0.8
2,672,899 - +0.5
2,673,302 + BWI76_RS12825 0.57 +0.4

Or see this region's nucleotide sequence