Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12670

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12655 and BWI76_RS12660 are separated by 177 nucleotidesBWI76_RS12660 and BWI76_RS12665 are separated by 154 nucleotidesBWI76_RS12665 and BWI76_RS12670 are separated by 275 nucleotidesBWI76_RS12670 and BWI76_RS12675 overlap by 1 nucleotides BWI76_RS12655: BWI76_RS12655 - PTS cellobiose transporter subunit IIB, at 2,640,329 to 2,640,631 _RS12655 BWI76_RS12660: BWI76_RS12660 - hypothetical protein, at 2,640,809 to 2,641,000 _RS12660 BWI76_RS12665: BWI76_RS12665 - hypothetical protein, at 2,641,155 to 2,641,349 _RS12665 BWI76_RS12670: BWI76_RS12670 - DNA polymerase V subunit UmuD, at 2,641,625 to 2,642,047 _RS12670 BWI76_RS12675: BWI76_RS12675 - putative UV protection and mutation protein, at 2,642,047 to 2,643,312 _RS12675 Position (kb) 2641 2642 2643Strain fitness (log2 ratio) -2 -1 0 1at 2640.633 kb on + strandat 2640.641 kb on - strandat 2640.641 kb on - strandat 2640.641 kb on - strandat 2640.641 kb on - strandat 2640.661 kb on + strandat 2640.661 kb on + strandat 2640.747 kb on + strandat 2640.781 kb on + strandat 2640.784 kb on + strandat 2640.784 kb on - strandat 2640.834 kb on - strand, within BWI76_RS12660at 2640.836 kb on - strand, within BWI76_RS12660at 2640.836 kb on - strand, within BWI76_RS12660at 2640.851 kb on + strand, within BWI76_RS12660at 2640.851 kb on + strand, within BWI76_RS12660at 2640.857 kb on - strand, within BWI76_RS12660at 2640.857 kb on - strand, within BWI76_RS12660at 2640.857 kb on - strand, within BWI76_RS12660at 2640.858 kb on - strand, within BWI76_RS12660at 2640.859 kb on - strand, within BWI76_RS12660at 2640.883 kb on - strand, within BWI76_RS12660at 2640.958 kb on - strand, within BWI76_RS12660at 2640.958 kb on - strand, within BWI76_RS12660at 2640.978 kb on - strand, within BWI76_RS12660at 2640.983 kb on + strandat 2640.985 kb on + strandat 2640.992 kb on - strandat 2640.993 kb on + strandat 2640.993 kb on + strandat 2640.993 kb on - strandat 2640.993 kb on - strandat 2640.995 kb on + strandat 2641.036 kb on - strandat 2641.036 kb on - strandat 2641.176 kb on + strand, within BWI76_RS12665at 2641.197 kb on - strand, within BWI76_RS12665at 2641.386 kb on - strandat 2641.386 kb on - strandat 2641.388 kb on + strandat 2641.396 kb on + strandat 2641.404 kb on - strandat 2641.423 kb on - strandat 2641.456 kb on + strandat 2641.456 kb on + strandat 2641.464 kb on - strandat 2641.808 kb on - strand, within BWI76_RS12670at 2641.981 kb on + strand, within BWI76_RS12670at 2642.053 kb on + strandat 2642.244 kb on + strand, within BWI76_RS12675at 2642.454 kb on + strand, within BWI76_RS12675at 2642.489 kb on - strand, within BWI76_RS12675at 2642.650 kb on + strand, within BWI76_RS12675at 2642.739 kb on + strand, within BWI76_RS12675at 2642.744 kb on + strand, within BWI76_RS12675at 2642.760 kb on + strand, within BWI76_RS12675at 2642.930 kb on - strand, within BWI76_RS12675

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,640,633 + -0.3
2,640,641 - -0.4
2,640,641 - -0.1
2,640,641 - +1.5
2,640,641 - -0.7
2,640,661 + +1.5
2,640,661 + -0.3
2,640,747 + -0.1
2,640,781 + -2.1
2,640,784 + +0.7
2,640,784 - -0.4
2,640,834 - BWI76_RS12660 0.13 -0.0
2,640,836 - BWI76_RS12660 0.14 +1.3
2,640,836 - BWI76_RS12660 0.14 +0.6
2,640,851 + BWI76_RS12660 0.22 +0.4
2,640,851 + BWI76_RS12660 0.22 -0.7
2,640,857 - BWI76_RS12660 0.25 +0.2
2,640,857 - BWI76_RS12660 0.25 -1.0
2,640,857 - BWI76_RS12660 0.25 +0.3
2,640,858 - BWI76_RS12660 0.26 -0.2
2,640,859 - BWI76_RS12660 0.26 -0.3
2,640,883 - BWI76_RS12660 0.39 -0.1
2,640,958 - BWI76_RS12660 0.78 +0.4
2,640,958 - BWI76_RS12660 0.78 +0.7
2,640,978 - BWI76_RS12660 0.88 +0.5
2,640,983 + +0.7
2,640,985 + +0.2
2,640,992 - -0.1
2,640,993 + +0.3
2,640,993 + -0.5
2,640,993 - -0.2
2,640,993 - -1.6
2,640,995 + -0.2
2,641,036 - -0.2
2,641,036 - +0.1
2,641,176 + BWI76_RS12665 0.11 -0.2
2,641,197 - BWI76_RS12665 0.22 -0.2
2,641,386 - -0.1
2,641,386 - +0.3
2,641,388 + -0.2
2,641,396 + -0.4
2,641,404 - +0.3
2,641,423 - -0.6
2,641,456 + -0.2
2,641,456 + +0.4
2,641,464 - -0.1
2,641,808 - BWI76_RS12670 0.43 +0.3
2,641,981 + BWI76_RS12670 0.84 +0.9
2,642,053 + +0.8
2,642,244 + BWI76_RS12675 0.16 -0.0
2,642,454 + BWI76_RS12675 0.32 +0.2
2,642,489 - BWI76_RS12675 0.35 -1.4
2,642,650 + BWI76_RS12675 0.48 -0.1
2,642,739 + BWI76_RS12675 0.55 +0.5
2,642,744 + BWI76_RS12675 0.55 -0.2
2,642,760 + BWI76_RS12675 0.56 -1.3
2,642,930 - BWI76_RS12675 0.70 +0.3

Or see this region's nucleotide sequence