Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12425

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12420 and BWI76_RS12425 are separated by 363 nucleotidesBWI76_RS12425 and BWI76_RS12430 are separated by 169 nucleotides BWI76_RS12420: BWI76_RS12420 - peptide ABC transporter substrate-binding protein SapA, at 2,594,005 to 2,595,648 _RS12420 BWI76_RS12425: BWI76_RS12425 - hypothetical protein, at 2,596,012 to 2,596,296 _RS12425 BWI76_RS12430: BWI76_RS12430 - AraC family transcriptional regulator, at 2,596,466 to 2,598,772 _RS12430 Position (kb) 2596 2597Strain fitness (log2 ratio) -1 0 1at 2595.881 kb on + strandat 2595.889 kb on - strandat 2595.889 kb on - strandat 2595.889 kb on - strandat 2596.004 kb on - strandat 2596.086 kb on - strand, within BWI76_RS12425at 2596.105 kb on - strand, within BWI76_RS12425at 2596.115 kb on - strand, within BWI76_RS12425at 2596.244 kb on + strand, within BWI76_RS12425at 2596.252 kb on - strand, within BWI76_RS12425at 2596.594 kb on + strandat 2596.728 kb on + strand, within BWI76_RS12430at 2597.157 kb on - strand, within BWI76_RS12430

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,595,881 + -0.1
2,595,889 - +0.1
2,595,889 - +0.1
2,595,889 - -0.2
2,596,004 - -0.1
2,596,086 - BWI76_RS12425 0.26 +0.6
2,596,105 - BWI76_RS12425 0.33 +0.7
2,596,115 - BWI76_RS12425 0.36 +0.3
2,596,244 + BWI76_RS12425 0.81 +1.7
2,596,252 - BWI76_RS12425 0.84 +0.5
2,596,594 + -0.3
2,596,728 + BWI76_RS12430 0.11 -0.2
2,597,157 - BWI76_RS12430 0.30 -0.5

Or see this region's nucleotide sequence