Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12170

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12155 and BWI76_RS12160 are separated by 57 nucleotidesBWI76_RS12160 and BWI76_RS12165 overlap by 1 nucleotidesBWI76_RS12165 and BWI76_RS12170 are separated by 2 nucleotidesBWI76_RS12170 and BWI76_RS12175 are separated by 130 nucleotides BWI76_RS12155: BWI76_RS12155 - hypothetical protein, at 2,548,628 to 2,548,849 _RS12155 BWI76_RS12160: BWI76_RS12160 - hypothetical protein, at 2,548,907 to 2,549,506 _RS12160 BWI76_RS12165: BWI76_RS12165 - hypothetical protein, at 2,549,506 to 2,549,712 _RS12165 BWI76_RS12170: BWI76_RS12170 - hypothetical protein, at 2,549,715 to 2,550,011 _RS12170 BWI76_RS12175: BWI76_RS12175 - hypothetical protein, at 2,550,142 to 2,550,825 _RS12175 Position (kb) 2549 2550 2551Strain fitness (log2 ratio) -1 0 1 2at 2548.801 kb on + strand, within BWI76_RS12155at 2548.801 kb on + strand, within BWI76_RS12155at 2548.878 kb on + strandat 2548.890 kb on + strandat 2548.898 kb on + strandat 2548.925 kb on - strandat 2548.934 kb on - strandat 2548.944 kb on - strandat 2548.956 kb on + strandat 2548.969 kb on - strand, within BWI76_RS12160at 2548.969 kb on - strand, within BWI76_RS12160at 2548.970 kb on - strand, within BWI76_RS12160at 2549.023 kb on + strand, within BWI76_RS12160at 2549.023 kb on + strand, within BWI76_RS12160at 2549.023 kb on + strand, within BWI76_RS12160at 2549.031 kb on - strand, within BWI76_RS12160at 2549.133 kb on + strand, within BWI76_RS12160at 2549.134 kb on + strand, within BWI76_RS12160at 2549.313 kb on - strand, within BWI76_RS12160at 2549.452 kb on - strandat 2549.937 kb on - strand, within BWI76_RS12170at 2549.963 kb on + strand, within BWI76_RS12170at 2549.989 kb on + strandat 2550.030 kb on + strandat 2550.058 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,548,801 + BWI76_RS12155 0.78 -0.8
2,548,801 + BWI76_RS12155 0.78 -0.5
2,548,878 + +0.2
2,548,890 + -0.1
2,548,898 + +0.1
2,548,925 - +0.5
2,548,934 - +1.9
2,548,944 - +0.1
2,548,956 + +0.7
2,548,969 - BWI76_RS12160 0.10 -0.4
2,548,969 - BWI76_RS12160 0.10 -0.2
2,548,970 - BWI76_RS12160 0.10 -0.0
2,549,023 + BWI76_RS12160 0.19 +0.2
2,549,023 + BWI76_RS12160 0.19 -0.6
2,549,023 + BWI76_RS12160 0.19 -0.1
2,549,031 - BWI76_RS12160 0.21 +0.1
2,549,133 + BWI76_RS12160 0.38 -0.7
2,549,134 + BWI76_RS12160 0.38 -0.1
2,549,313 - BWI76_RS12160 0.68 +0.1
2,549,452 - -0.1
2,549,937 - BWI76_RS12170 0.75 +0.9
2,549,963 + BWI76_RS12170 0.84 +0.6
2,549,989 + -0.9
2,550,030 + +0.0
2,550,058 - +0.1

Or see this region's nucleotide sequence