Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11515

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11505 and BWI76_RS11510 overlap by 47 nucleotidesBWI76_RS11510 and BWI76_RS11515 are separated by 188 nucleotidesBWI76_RS11515 and BWI76_RS11520 are separated by 42 nucleotidesBWI76_RS11520 and BWI76_RS11525 are separated by 60 nucleotidesBWI76_RS11525 and BWI76_RS11530 are separated by 100 nucleotides BWI76_RS11505: BWI76_RS11505 - aldehyde dehydrogenase, at 2,422,483 to 2,423,478 _RS11505 BWI76_RS11510: BWI76_RS11510 - hypothetical protein, at 2,423,432 to 2,423,628 _RS11510 BWI76_RS11515: BWI76_RS11515 - peptide-methionine (R)-S-oxide reductase, at 2,423,817 to 2,424,230 _RS11515 BWI76_RS11520: BWI76_RS11520 - hypothetical protein, at 2,424,273 to 2,424,548 _RS11520 BWI76_RS11525: BWI76_RS11525 - HNH endonuclease, at 2,424,609 to 2,424,953 _RS11525 BWI76_RS11530: BWI76_RS11530 - chitinase, at 2,425,054 to 2,426,307 _RS11530 Position (kb) 2423 2424 2425Strain fitness (log2 ratio) -1 0 1at 2423.731 kb on + strandat 2423.841 kb on - strandat 2423.870 kb on + strand, within BWI76_RS11515at 2423.901 kb on - strand, within BWI76_RS11515at 2424.443 kb on + strand, within BWI76_RS11520at 2424.573 kb on + strandat 2424.759 kb on + strand, within BWI76_RS11525at 2424.772 kb on - strand, within BWI76_RS11525at 2425.060 kb on - strandat 2425.203 kb on + strand, within BWI76_RS11530

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,423,731 + +0.2
2,423,841 - +0.9
2,423,870 + BWI76_RS11515 0.13 -0.3
2,423,901 - BWI76_RS11515 0.20 +1.0
2,424,443 + BWI76_RS11520 0.62 -0.3
2,424,573 + -0.2
2,424,759 + BWI76_RS11525 0.43 -0.1
2,424,772 - BWI76_RS11525 0.47 -0.4
2,425,060 - +0.4
2,425,203 + BWI76_RS11530 0.12 +0.5

Or see this region's nucleotide sequence