Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11505 and BWI76_RS11510 overlap by 47 nucleotides BWI76_RS11510 and BWI76_RS11515 are separated by 188 nucleotides BWI76_RS11515 and BWI76_RS11520 are separated by 42 nucleotides BWI76_RS11520 and BWI76_RS11525 are separated by 60 nucleotides BWI76_RS11525 and BWI76_RS11530 are separated by 100 nucleotides
BWI76_RS11505: BWI76_RS11505 - aldehyde dehydrogenase, at 2,422,483 to 2,423,478
_RS11505
BWI76_RS11510: BWI76_RS11510 - hypothetical protein, at 2,423,432 to 2,423,628
_RS11510
BWI76_RS11515: BWI76_RS11515 - peptide-methionine (R)-S-oxide reductase, at 2,423,817 to 2,424,230
_RS11515
BWI76_RS11520: BWI76_RS11520 - hypothetical protein, at 2,424,273 to 2,424,548
_RS11520
BWI76_RS11525: BWI76_RS11525 - HNH endonuclease, at 2,424,609 to 2,424,953
_RS11525
BWI76_RS11530: BWI76_RS11530 - chitinase, at 2,425,054 to 2,426,307
_RS11530
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-1
0
1 at 2423.731 kb on + strand at 2423.841 kb on - strand at 2423.870 kb on + strand, within BWI76_RS11515 at 2423.901 kb on - strand, within BWI76_RS11515 at 2424.443 kb on + strand, within BWI76_RS11520 at 2424.573 kb on + strand at 2424.759 kb on + strand, within BWI76_RS11525 at 2424.772 kb on - strand, within BWI76_RS11525 at 2425.060 kb on - strand at 2425.203 kb on + strand, within BWI76_RS11530
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,423,731 + +0.2 2,423,841 - +0.9 2,423,870 + BWI76_RS11515 0.13 -0.3 2,423,901 - BWI76_RS11515 0.20 +1.0 2,424,443 + BWI76_RS11520 0.62 -0.3 2,424,573 + -0.2 2,424,759 + BWI76_RS11525 0.43 -0.1 2,424,772 - BWI76_RS11525 0.47 -0.4 2,425,060 - +0.4 2,425,203 + BWI76_RS11530 0.12 +0.5
Or see this region's nucleotide sequence