Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11150

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11135 and BWI76_RS11140 are separated by 103 nucleotidesBWI76_RS11140 and BWI76_RS11145 overlap by 2 nucleotidesBWI76_RS11145 and BWI76_RS11150 are separated by 5 nucleotidesBWI76_RS11150 and BWI76_RS11155 are separated by 12 nucleotidesBWI76_RS11155 and BWI76_RS11160 overlap by 20 nucleotides BWI76_RS11135: BWI76_RS11135 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 2,348,097 to 2,350,283 _RS11135 BWI76_RS11140: BWI76_RS11140 - hypothetical protein, at 2,350,387 to 2,350,635 _RS11140 BWI76_RS11145: BWI76_RS11145 - histidine triad nucleotide-binding protein, at 2,350,634 to 2,350,990 _RS11145 BWI76_RS11150: BWI76_RS11150 - membrane protein, at 2,350,996 to 2,351,370 _RS11150 BWI76_RS11155: BWI76_RS11155 - penicillin-binding protein activator LpoB, at 2,351,383 to 2,352,018 _RS11155 BWI76_RS11160: BWI76_RS11160 - thiamine kinase, at 2,351,999 to 2,352,817 _RS11160 Position (kb) 2350 2351 2352Strain fitness (log2 ratio) -1 0 1at 2351.192 kb on - strand, within BWI76_RS11150at 2351.319 kb on - strand, within BWI76_RS11150at 2351.400 kb on - strandat 2351.658 kb on - strand, within BWI76_RS11155at 2351.881 kb on - strand, within BWI76_RS11155at 2352.007 kb on - strandat 2352.066 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,351,192 - BWI76_RS11150 0.52 +0.9
2,351,319 - BWI76_RS11150 0.86 +0.7
2,351,400 - -0.5
2,351,658 - BWI76_RS11155 0.43 +0.1
2,351,881 - BWI76_RS11155 0.78 +0.0
2,352,007 - -0.3
2,352,066 - -0.5

Or see this region's nucleotide sequence