Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11125

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11120 and BWI76_RS11125 are separated by 10 nucleotidesBWI76_RS11125 and BWI76_RS11130 are separated by 297 nucleotides BWI76_RS11120: BWI76_RS11120 - DNA polymerase III subunit delta', at 2,344,454 to 2,345,458 _RS11120 BWI76_RS11125: BWI76_RS11125 - metal-dependent hydrolase, at 2,345,469 to 2,346,263 _RS11125 BWI76_RS11130: BWI76_RS11130 - PTS glucose transporter subunit IIBC, at 2,346,561 to 2,347,994 _RS11130 Position (kb) 2345 2346 2347Strain fitness (log2 ratio) -1 0 1at 2346.003 kb on + strand, within BWI76_RS11125at 2346.283 kb on - strandat 2346.475 kb on + strandat 2346.725 kb on - strand, within BWI76_RS11130at 2346.881 kb on - strand, within BWI76_RS11130at 2347.171 kb on + strand, within BWI76_RS11130

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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2,346,003 + BWI76_RS11125 0.67 +0.6
2,346,283 - +0.2
2,346,475 + -0.3
2,346,725 - BWI76_RS11130 0.11 +0.1
2,346,881 - BWI76_RS11130 0.22 +0.1
2,347,171 + BWI76_RS11130 0.43 -0.3

Or see this region's nucleotide sequence