Experiment: 2-Deoxyadenosine 4 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10570 and BWI76_RS10575 are separated by 9 nucleotides BWI76_RS10575 and BWI76_RS10580 overlap by 1 nucleotides BWI76_RS10580 and BWI76_RS10585 overlap by 20 nucleotides BWI76_RS10585 and BWI76_RS10590 are separated by 10 nucleotides
BWI76_RS10570: BWI76_RS10570 - cysteine ABC transporter substrate-binding protein, at 2,232,870 to 2,233,655
_RS10570
BWI76_RS10575: BWI76_RS10575 - polar amino acid ABC transporter permease, at 2,233,665 to 2,234,330
_RS10575
BWI76_RS10580: BWI76_RS10580 - amino acid ABC transporter permease, at 2,234,330 to 2,234,986
_RS10580
BWI76_RS10585: BWI76_RS10585 - amino acid ABC transporter ATP-binding protein, at 2,234,967 to 2,235,707
_RS10585
BWI76_RS10590: BWI76_RS10590 - class V aminotransferase, at 2,235,718 to 2,236,959
_RS10590
Position (kb)
2234
2235 Strain fitness (log2 ratio)
-1
0
1 at 2233.384 kb on - strand, within BWI76_RS10570 at 2233.713 kb on - strand at 2233.851 kb on - strand, within BWI76_RS10575 at 2233.978 kb on + strand, within BWI76_RS10575 at 2234.275 kb on - strand at 2234.506 kb on + strand, within BWI76_RS10580 at 2234.514 kb on - strand, within BWI76_RS10580 at 2234.855 kb on - strand, within BWI76_RS10580 at 2235.150 kb on - strand at 2235.161 kb on + strand, within BWI76_RS10585 at 2235.391 kb on + strand, within BWI76_RS10585 at 2235.650 kb on + strand at 2235.709 kb on + strand at 2235.765 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C) remove 2,233,384 - BWI76_RS10570 0.65 +0.2 2,233,713 - -0.2 2,233,851 - BWI76_RS10575 0.28 -0.0 2,233,978 + BWI76_RS10575 0.47 -1.3 2,234,275 - +1.3 2,234,506 + BWI76_RS10580 0.27 +0.5 2,234,514 - BWI76_RS10580 0.28 +0.8 2,234,855 - BWI76_RS10580 0.80 +0.3 2,235,150 - +1.7 2,235,161 + BWI76_RS10585 0.26 +0.4 2,235,391 + BWI76_RS10585 0.57 +0.4 2,235,650 + +0.2 2,235,709 + -1.4 2,235,765 + +0.8
Or see this region's nucleotide sequence