Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09220

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09205 and BWI76_RS09210 are separated by 125 nucleotidesBWI76_RS09210 and BWI76_RS09215 are separated by 32 nucleotidesBWI76_RS09215 and BWI76_RS09220 are separated by 6 nucleotidesBWI76_RS09220 and BWI76_RS09225 overlap by 37 nucleotidesBWI76_RS09225 and BWI76_RS09230 are separated by 67 nucleotidesBWI76_RS09230 and BWI76_RS09235 overlap by 1 nucleotidesBWI76_RS09235 and BWI76_RS09240 overlap by 4 nucleotides BWI76_RS09205: BWI76_RS09205 - phage repressor protein CI, at 1,942,612 to 1,943,172 _RS09205 BWI76_RS09210: BWI76_RS09210 - hypothetical protein, at 1,943,298 to 1,943,519 _RS09210 BWI76_RS09215: BWI76_RS09215 - phage regulatory CII family protein, at 1,943,552 to 1,944,061 _RS09215 BWI76_RS09220: BWI76_RS09220 - hypothetical protein, at 1,944,068 to 1,944,268 _RS09220 BWI76_RS09225: BWI76_RS09225 - hypothetical protein, at 1,944,232 to 1,944,573 _RS09225 BWI76_RS09230: BWI76_RS09230 - hypothetical protein, at 1,944,641 to 1,944,877 _RS09230 BWI76_RS09235: BWI76_RS09235 - TraR/DksA family transcriptional regulator, at 1,944,877 to 1,945,104 _RS09235 BWI76_RS09240: BWI76_RS09240 - phosphoadenosine phosphosulfate reductase, at 1,945,101 to 1,946,111 _RS09240 Position (kb) 1944 1945Strain fitness (log2 ratio) -1 0 1 2at 1943.623 kb on - strand, within BWI76_RS09215at 1943.625 kb on - strand, within BWI76_RS09215at 1943.651 kb on - strand, within BWI76_RS09215at 1943.656 kb on - strand, within BWI76_RS09215at 1943.656 kb on - strand, within BWI76_RS09215at 1943.666 kb on - strand, within BWI76_RS09215at 1943.682 kb on + strand, within BWI76_RS09215at 1943.685 kb on - strand, within BWI76_RS09215at 1943.685 kb on - strand, within BWI76_RS09215at 1943.685 kb on - strandat 1943.690 kb on - strand, within BWI76_RS09215at 1943.818 kb on + strand, within BWI76_RS09215at 1943.848 kb on - strand, within BWI76_RS09215at 1943.860 kb on - strand, within BWI76_RS09215at 1943.967 kb on + strand, within BWI76_RS09215at 1944.063 kb on - strandat 1944.068 kb on - strandat 1944.078 kb on - strandat 1944.131 kb on + strand, within BWI76_RS09220at 1944.154 kb on + strand, within BWI76_RS09220at 1944.155 kb on + strand, within BWI76_RS09220at 1944.178 kb on - strand, within BWI76_RS09220at 1944.198 kb on + strand, within BWI76_RS09220at 1944.206 kb on - strand, within BWI76_RS09220at 1944.221 kb on - strand, within BWI76_RS09220at 1944.246 kb on - strandat 1944.259 kb on + strandat 1944.267 kb on - strandat 1944.267 kb on - strandat 1944.269 kb on + strand, within BWI76_RS09225at 1944.269 kb on + strand, within BWI76_RS09225at 1944.269 kb on + strand, within BWI76_RS09225at 1944.274 kb on + strandat 1944.277 kb on - strand, within BWI76_RS09225at 1944.277 kb on - strand, within BWI76_RS09225at 1944.290 kb on + strand, within BWI76_RS09225at 1944.302 kb on - strand, within BWI76_RS09225at 1944.322 kb on - strand, within BWI76_RS09225at 1944.469 kb on - strand, within BWI76_RS09225at 1944.478 kb on - strand, within BWI76_RS09225at 1944.485 kb on - strand, within BWI76_RS09225at 1944.485 kb on - strand, within BWI76_RS09225at 1944.490 kb on - strand, within BWI76_RS09225at 1944.502 kb on - strand, within BWI76_RS09225at 1944.504 kb on + strand, within BWI76_RS09225at 1944.504 kb on + strand, within BWI76_RS09225at 1944.512 kb on - strand, within BWI76_RS09225at 1944.512 kb on - strand, within BWI76_RS09225at 1944.540 kb on - strandat 1944.804 kb on + strand, within BWI76_RS09230at 1944.812 kb on - strand, within BWI76_RS09230at 1944.852 kb on - strand, within BWI76_RS09230at 1944.893 kb on + strandat 1944.896 kb on + strandat 1945.182 kb on - strandat 1945.182 kb on - strandat 1945.218 kb on - strand, within BWI76_RS09240

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,943,623 - BWI76_RS09215 0.14 +0.5
1,943,625 - BWI76_RS09215 0.14 +0.5
1,943,651 - BWI76_RS09215 0.19 -0.7
1,943,656 - BWI76_RS09215 0.20 +0.3
1,943,656 - BWI76_RS09215 0.20 +0.1
1,943,666 - BWI76_RS09215 0.22 -0.1
1,943,682 + BWI76_RS09215 0.25 -0.6
1,943,685 - BWI76_RS09215 0.26 +0.4
1,943,685 - BWI76_RS09215 0.26 +0.0
1,943,685 - +0.2
1,943,690 - BWI76_RS09215 0.27 -1.6
1,943,818 + BWI76_RS09215 0.52 +0.6
1,943,848 - BWI76_RS09215 0.58 -0.9
1,943,860 - BWI76_RS09215 0.60 -0.3
1,943,967 + BWI76_RS09215 0.81 -0.5
1,944,063 - -0.5
1,944,068 - +0.3
1,944,078 - +0.2
1,944,131 + BWI76_RS09220 0.31 +0.5
1,944,154 + BWI76_RS09220 0.43 +0.3
1,944,155 + BWI76_RS09220 0.43 +0.2
1,944,178 - BWI76_RS09220 0.55 +2.2
1,944,198 + BWI76_RS09220 0.65 +0.8
1,944,206 - BWI76_RS09220 0.69 +0.1
1,944,221 - BWI76_RS09220 0.76 +0.8
1,944,246 - -0.5
1,944,259 + -1.0
1,944,267 - -0.3
1,944,267 - -0.0
1,944,269 + BWI76_RS09225 0.11 +0.1
1,944,269 + BWI76_RS09225 0.11 -0.5
1,944,269 + BWI76_RS09225 0.11 -1.6
1,944,274 + -0.8
1,944,277 - BWI76_RS09225 0.13 +0.7
1,944,277 - BWI76_RS09225 0.13 +0.5
1,944,290 + BWI76_RS09225 0.17 -0.4
1,944,302 - BWI76_RS09225 0.20 +0.6
1,944,322 - BWI76_RS09225 0.26 -0.3
1,944,469 - BWI76_RS09225 0.69 +0.3
1,944,478 - BWI76_RS09225 0.72 -0.3
1,944,485 - BWI76_RS09225 0.74 +0.8
1,944,485 - BWI76_RS09225 0.74 -0.5
1,944,490 - BWI76_RS09225 0.75 -0.4
1,944,502 - BWI76_RS09225 0.79 +0.8
1,944,504 + BWI76_RS09225 0.80 +1.1
1,944,504 + BWI76_RS09225 0.80 -0.0
1,944,512 - BWI76_RS09225 0.82 +0.3
1,944,512 - BWI76_RS09225 0.82 -0.2
1,944,540 - +0.2
1,944,804 + BWI76_RS09230 0.69 +0.2
1,944,812 - BWI76_RS09230 0.72 -0.1
1,944,852 - BWI76_RS09230 0.89 +0.5
1,944,893 + +0.3
1,944,896 + +0.8
1,945,182 - +0.1
1,945,182 - -1.0
1,945,218 - BWI76_RS09240 0.12 +0.1

Or see this region's nucleotide sequence