Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08275

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08265 and BWI76_RS08270 are separated by 291 nucleotidesBWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotidesBWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides BWI76_RS08265: BWI76_RS08265 - transcriptional repressor, at 1,750,135 to 1,750,587 _RS08265 BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508 _RS08270 BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852 _RS08275 BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759 _RS08280 Position (kb) 1751 1752Strain fitness (log2 ratio) -1 0 1at 1750.688 kb on + strandat 1750.810 kb on + strandat 1751.518 kb on + strandat 1751.526 kb on - strandat 1751.571 kb on - strandat 1751.722 kb on + strand, within BWI76_RS08275at 1751.754 kb on - strand, within BWI76_RS08275at 1752.008 kb on - strandat 1752.163 kb on - strand, within BWI76_RS08280at 1752.589 kb on + strand, within BWI76_RS08280at 1752.605 kb on - strand, within BWI76_RS08280

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,750,688 + +0.5
1,750,810 + +0.1
1,751,518 + +0.5
1,751,526 - -0.5
1,751,571 - -0.3
1,751,722 + BWI76_RS08275 0.55 +0.1
1,751,754 - BWI76_RS08275 0.66 +1.0
1,752,008 - +0.5
1,752,163 - BWI76_RS08280 0.23 -0.1
1,752,589 + BWI76_RS08280 0.78 +0.0
1,752,605 - BWI76_RS08280 0.80 -0.6

Or see this region's nucleotide sequence