Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS07730

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS07720 and BWI76_RS07725 overlap by 4 nucleotidesBWI76_RS07725 and BWI76_RS07730 overlap by 4 nucleotidesBWI76_RS07730 and BWI76_RS07735 are separated by 112 nucleotides BWI76_RS07720: BWI76_RS07720 - iron-enterobactin transporter ATP-binding protein, at 1,637,776 to 1,638,570 _RS07720 BWI76_RS07725: BWI76_RS07725 - iron-enterobactin transporter permease, at 1,638,567 to 1,639,559 _RS07725 BWI76_RS07730: BWI76_RS07730 - iron-enterobactin transporter membrane protein, at 1,639,556 to 1,640,563 _RS07730 BWI76_RS07735: BWI76_RS07735 - MFS transporter, at 1,640,676 to 1,641,917 _RS07735 Position (kb) 1639 1640 1641Strain fitness (log2 ratio) -1 0 1 2at 1638.661 kb on + strandat 1639.293 kb on + strand, within BWI76_RS07725at 1640.043 kb on + strand, within BWI76_RS07730at 1640.527 kb on + strandat 1640.628 kb on + strandat 1640.681 kb on - strandat 1641.025 kb on + strand, within BWI76_RS07735at 1641.368 kb on + strand, within BWI76_RS07735at 1641.376 kb on - strand, within BWI76_RS07735at 1641.389 kb on + strand, within BWI76_RS07735at 1641.498 kb on + strand, within BWI76_RS07735

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,638,661 + +1.6
1,639,293 + BWI76_RS07725 0.73 +0.4
1,640,043 + BWI76_RS07730 0.48 +1.2
1,640,527 + +0.3
1,640,628 + +1.0
1,640,681 - -0.1
1,641,025 + BWI76_RS07735 0.28 -0.1
1,641,368 + BWI76_RS07735 0.56 +1.0
1,641,376 - BWI76_RS07735 0.56 +2.0
1,641,389 + BWI76_RS07735 0.57 +0.3
1,641,498 + BWI76_RS07735 0.66 +0.4

Or see this region's nucleotide sequence