Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05075

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotidesBWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotidesBWI76_RS05070 and BWI76_RS05075 overlap by 1 nucleotidesBWI76_RS05075 and BWI76_RS05080 are separated by 20 nucleotides BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271 _RS05060 BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633 _RS05065 BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142 _RS05070 BWI76_RS05075: BWI76_RS05075 - type II secretion system protein GspG, at 1,062,142 to 1,062,564 _RS05075 BWI76_RS05080: BWI76_RS05080 - type II secretion protein F, at 1,062,585 to 1,063,790 _RS05080 Position (kb) 1062 1063Strain fitness (log2 ratio) -1 0 1at 1061.244 kb on + strandat 1061.244 kb on + strandat 1061.244 kb on + strandat 1061.252 kb on - strandat 1061.451 kb on - strand, within BWI76_RS05065at 1061.780 kb on - strand, within BWI76_RS05070at 1062.031 kb on - strand, within BWI76_RS05070at 1062.129 kb on - strandat 1062.201 kb on + strand, within BWI76_RS05075at 1062.687 kb on - strandat 1062.687 kb on - strandat 1062.687 kb on - strandat 1062.845 kb on + strand, within BWI76_RS05080at 1062.942 kb on - strand, within BWI76_RS05080at 1063.081 kb on + strand, within BWI76_RS05080at 1063.193 kb on + strand, within BWI76_RS05080at 1063.279 kb on - strand, within BWI76_RS05080at 1063.339 kb on - strand, within BWI76_RS05080at 1063.339 kb on - strand, within BWI76_RS05080at 1063.410 kb on - strand, within BWI76_RS05080

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,061,244 + -0.8
1,061,244 + -0.1
1,061,244 + +0.5
1,061,252 - -1.2
1,061,451 - BWI76_RS05065 0.50 -0.9
1,061,780 - BWI76_RS05070 0.29 +0.0
1,062,031 - BWI76_RS05070 0.78 +0.4
1,062,129 - -0.0
1,062,201 + BWI76_RS05075 0.14 +0.7
1,062,687 - -0.2
1,062,687 - +0.1
1,062,687 - +0.0
1,062,845 + BWI76_RS05080 0.22 +0.6
1,062,942 - BWI76_RS05080 0.30 -0.4
1,063,081 + BWI76_RS05080 0.41 +1.1
1,063,193 + BWI76_RS05080 0.50 +0.4
1,063,279 - BWI76_RS05080 0.58 +0.0
1,063,339 - BWI76_RS05080 0.63 +0.2
1,063,339 - BWI76_RS05080 0.63 +0.5
1,063,410 - BWI76_RS05080 0.68 +0.1

Or see this region's nucleotide sequence