Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05050

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05040 and BWI76_RS05045 overlap by 11 nucleotidesBWI76_RS05045 and BWI76_RS05050 overlap by 8 nucleotidesBWI76_RS05050 and BWI76_RS05055 overlap by 8 nucleotidesBWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotides BWI76_RS05040: BWI76_RS05040 - type II secretion system protein N, at 1,057,283 to 1,058,041 _RS05040 BWI76_RS05045: BWI76_RS05045 - general secretion pathway protein GspM, at 1,058,031 to 1,058,516 _RS05045 BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705 _RS05050 BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678 _RS05055 BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271 _RS05060 Position (kb) 1058 1059 1060Strain fitness (log2 ratio) -2 -1 0 1at 1057.520 kb on + strand, within BWI76_RS05040at 1057.946 kb on - strand, within BWI76_RS05040at 1058.457 kb on - strand, within BWI76_RS05045at 1058.457 kb on - strand, within BWI76_RS05045at 1058.541 kb on + strandat 1058.616 kb on + strandat 1058.624 kb on - strandat 1058.624 kb on - strandat 1059.018 kb on + strand, within BWI76_RS05050at 1059.306 kb on + strand, within BWI76_RS05050at 1059.306 kb on + strand, within BWI76_RS05050at 1059.533 kb on - strand, within BWI76_RS05050at 1059.797 kb on + strand, within BWI76_RS05055at 1059.970 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.020 kb on + strand, within BWI76_RS05055at 1060.106 kb on - strand, within BWI76_RS05055at 1060.181 kb on - strand, within BWI76_RS05055at 1060.242 kb on + strand, within BWI76_RS05055at 1060.267 kb on + strand, within BWI76_RS05055at 1060.311 kb on + strand, within BWI76_RS05055at 1060.348 kb on + strand, within BWI76_RS05055at 1060.371 kb on - strand, within BWI76_RS05055at 1060.438 kb on + strandat 1060.445 kb on + strand, within BWI76_RS05055at 1060.453 kb on - strand, within BWI76_RS05055at 1060.607 kb on + strandat 1060.649 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,057,520 + BWI76_RS05040 0.31 -0.5
1,057,946 - BWI76_RS05040 0.87 -1.0
1,058,457 - BWI76_RS05045 0.88 -1.0
1,058,457 - BWI76_RS05045 0.88 -0.0
1,058,541 + -0.5
1,058,616 + +1.1
1,058,624 - +0.6
1,058,624 - +0.6
1,059,018 + BWI76_RS05050 0.43 +1.6
1,059,306 + BWI76_RS05050 0.67 +0.5
1,059,306 + BWI76_RS05050 0.67 +0.2
1,059,533 - BWI76_RS05050 0.86 +0.7
1,059,797 + BWI76_RS05055 0.10 +0.1
1,059,970 + BWI76_RS05055 0.28 +0.2
1,060,020 + BWI76_RS05055 0.33 -0.6
1,060,020 + BWI76_RS05055 0.33 -0.1
1,060,020 + BWI76_RS05055 0.33 -1.0
1,060,106 - BWI76_RS05055 0.42 +0.3
1,060,181 - BWI76_RS05055 0.49 +0.3
1,060,242 + BWI76_RS05055 0.55 +0.7
1,060,267 + BWI76_RS05055 0.58 -0.2
1,060,311 + BWI76_RS05055 0.62 -1.8
1,060,348 + BWI76_RS05055 0.66 +0.8
1,060,371 - BWI76_RS05055 0.69 +0.1
1,060,438 + +1.6
1,060,445 + BWI76_RS05055 0.76 +0.8
1,060,453 - BWI76_RS05055 0.77 +0.5
1,060,607 + +0.3
1,060,649 - +0.0

Or see this region's nucleotide sequence