Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04975

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04965 and BWI76_RS04970 overlap by 4 nucleotidesBWI76_RS04970 and BWI76_RS04975 are separated by 121 nucleotidesBWI76_RS04975 and BWI76_RS04980 are separated by 57 nucleotides BWI76_RS04965: BWI76_RS04965 - ABC transporter ATP-binding protein, at 1,042,941 to 1,043,867 _RS04965 BWI76_RS04970: BWI76_RS04970 - ABC transporter permease, at 1,043,864 to 1,044,634 _RS04970 BWI76_RS04975: BWI76_RS04975 - PTS fructose subfamily transporter subunit IIA, at 1,044,756 to 1,045,196 _RS04975 BWI76_RS04980: BWI76_RS04980 - hypothetical protein, at 1,045,254 to 1,046,507 _RS04980 Position (kb) 1044 1045 1046Strain fitness (log2 ratio) -3 -2 -1 0 1at 1043.784 kb on - strandat 1044.143 kb on - strand, within BWI76_RS04970at 1044.297 kb on + strand, within BWI76_RS04970at 1044.305 kb on - strand, within BWI76_RS04970at 1044.654 kb on + strandat 1044.842 kb on + strand, within BWI76_RS04975at 1044.959 kb on - strand, within BWI76_RS04975at 1045.356 kb on - strandat 1045.436 kb on - strand, within BWI76_RS04980at 1045.514 kb on - strand, within BWI76_RS04980at 1045.691 kb on + strand, within BWI76_RS04980at 1045.699 kb on - strand, within BWI76_RS04980at 1045.904 kb on + strand, within BWI76_RS04980at 1045.912 kb on - strand, within BWI76_RS04980at 1045.912 kb on - strand, within BWI76_RS04980at 1046.015 kb on + strand, within BWI76_RS04980at 1046.017 kb on - strand, within BWI76_RS04980

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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1,043,784 - +0.1
1,044,143 - BWI76_RS04970 0.36 -0.2
1,044,297 + BWI76_RS04970 0.56 -0.2
1,044,305 - BWI76_RS04970 0.57 -2.9
1,044,654 + +1.4
1,044,842 + BWI76_RS04975 0.20 +0.3
1,044,959 - BWI76_RS04975 0.46 +1.0
1,045,356 - -1.2
1,045,436 - BWI76_RS04980 0.15 -1.7
1,045,514 - BWI76_RS04980 0.21 +0.1
1,045,691 + BWI76_RS04980 0.35 +1.5
1,045,699 - BWI76_RS04980 0.35 -1.1
1,045,904 + BWI76_RS04980 0.52 -2.0
1,045,912 - BWI76_RS04980 0.52 -0.8
1,045,912 - BWI76_RS04980 0.52 +0.1
1,046,015 + BWI76_RS04980 0.61 +0.2
1,046,017 - BWI76_RS04980 0.61 -1.0

Or see this region's nucleotide sequence