Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02190

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotidesBWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotidesBWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375 _RS02180 BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167 _RS02185 BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745 _RS02190 BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881 _RS02195 Position (kb) 454 455Strain fitness (log2 ratio) -1 0 1at 453.224 kb on - strand, within BWI76_RS02180at 453.304 kb on + strand, within BWI76_RS02180at 453.504 kb on + strandat 453.537 kb on + strand, within BWI76_RS02185at 453.705 kb on - strand, within BWI76_RS02185at 453.803 kb on - strand, within BWI76_RS02185at 453.803 kb on - strand, within BWI76_RS02185at 453.830 kb on + strand, within BWI76_RS02185at 453.848 kb on - strand, within BWI76_RS02185at 453.943 kb on + strand, within BWI76_RS02185at 454.169 kb on - strandat 454.274 kb on + strand, within BWI76_RS02190at 454.343 kb on + strand, within BWI76_RS02190at 454.559 kb on + strand, within BWI76_RS02190at 454.695 kb on + strandat 454.695 kb on + strandat 454.695 kb on + strandat 454.695 kb on + strandat 454.763 kb on - strandat 454.797 kb on + strandat 454.920 kb on - strandat 454.931 kb on + strand, within BWI76_RS02195at 455.083 kb on - strand, within BWI76_RS02195at 455.091 kb on - strand, within BWI76_RS02195at 455.298 kb on - strand, within BWI76_RS02195at 455.367 kb on + strand, within BWI76_RS02195at 455.479 kb on + strand, within BWI76_RS02195at 455.618 kb on - strand, within BWI76_RS02195at 455.656 kb on - strand, within BWI76_RS02195at 455.667 kb on + strand, within BWI76_RS02195at 455.680 kb on - strand, within BWI76_RS02195

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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453,224 - BWI76_RS02180 0.54 +1.0
453,304 + BWI76_RS02180 0.78 -1.5
453,504 + +1.0
453,537 + BWI76_RS02185 0.17 -0.1
453,705 - BWI76_RS02185 0.39 -0.2
453,803 - BWI76_RS02185 0.52 -0.1
453,803 - BWI76_RS02185 0.52 -0.5
453,830 + BWI76_RS02185 0.55 -0.1
453,848 - BWI76_RS02185 0.58 +0.4
453,943 + BWI76_RS02185 0.70 -1.1
454,169 - -0.6
454,274 + BWI76_RS02190 0.16 -0.3
454,343 + BWI76_RS02190 0.29 +1.5
454,559 + BWI76_RS02190 0.67 +0.6
454,695 + -0.3
454,695 + +0.0
454,695 + -0.4
454,695 + +0.5
454,763 - +0.0
454,797 + -0.5
454,920 - +0.3
454,931 + BWI76_RS02195 0.16 -0.8
455,083 - BWI76_RS02195 0.30 +0.8
455,091 - BWI76_RS02195 0.30 -0.1
455,298 - BWI76_RS02195 0.49 -0.0
455,367 + BWI76_RS02195 0.55 +0.1
455,479 + BWI76_RS02195 0.65 +0.4
455,618 - BWI76_RS02195 0.77 -0.7
455,656 - BWI76_RS02195 0.80 -0.6
455,667 + BWI76_RS02195 0.81 -0.3
455,680 - BWI76_RS02195 0.82 +0.2

Or see this region's nucleotide sequence