Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01935

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01930 and BWI76_RS01935 are separated by 141 nucleotidesBWI76_RS01935 and BWI76_RS01940 are separated by 2 nucleotidesBWI76_RS01940 and BWI76_RS01945 are separated by 191 nucleotidesBWI76_RS01945 and BWI76_RS01950 are separated by 132 nucleotides BWI76_RS01930: BWI76_RS01930 - acid phosphatase/phosphotransferase, at 393,687 to 394,400 _RS01930 BWI76_RS01935: BWI76_RS01935 - thiamin phosphate synthase, at 394,542 to 394,958 _RS01935 BWI76_RS01940: BWI76_RS01940 - hypothetical protein, at 394,961 to 395,314 _RS01940 BWI76_RS01945: BWI76_RS01945 - hypothetical protein, at 395,506 to 395,753 _RS01945 BWI76_RS01950: BWI76_RS01950 - excinuclease ABC subunit A, at 395,886 to 398,711 _RS01950 Position (kb) 394 395Strain fitness (log2 ratio) -2 -1 0 1 2 3at 393.583 kb on - strandat 393.699 kb on + strandat 393.706 kb on + strandat 393.823 kb on - strand, within BWI76_RS01930at 393.823 kb on - strand, within BWI76_RS01930at 393.838 kb on - strand, within BWI76_RS01930at 394.227 kb on + strand, within BWI76_RS01930at 394.285 kb on + strand, within BWI76_RS01930at 394.369 kb on + strandat 394.377 kb on - strandat 394.473 kb on + strandat 394.488 kb on - strandat 394.560 kb on - strandat 394.637 kb on + strand, within BWI76_RS01935at 394.858 kb on - strand, within BWI76_RS01935at 394.862 kb on - strand, within BWI76_RS01935at 394.934 kb on - strandat 394.994 kb on + strandat 395.004 kb on + strand, within BWI76_RS01940at 395.009 kb on + strand, within BWI76_RS01940at 395.044 kb on + strand, within BWI76_RS01940at 395.321 kb on + strandat 395.347 kb on - strandat 395.442 kb on + strandat 395.529 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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393,583 - +0.5
393,699 + -1.8
393,706 + +0.6
393,823 - BWI76_RS01930 0.19 +0.4
393,823 - BWI76_RS01930 0.19 +0.2
393,838 - BWI76_RS01930 0.21 +0.6
394,227 + BWI76_RS01930 0.76 +3.4
394,285 + BWI76_RS01930 0.84 +0.9
394,369 + +0.1
394,377 - -0.3
394,473 + +0.2
394,488 - +0.0
394,560 - +0.3
394,637 + BWI76_RS01935 0.23 +0.9
394,858 - BWI76_RS01935 0.76 -1.3
394,862 - BWI76_RS01935 0.77 +1.0
394,934 - -0.4
394,994 + -0.2
395,004 + BWI76_RS01940 0.12 -0.4
395,009 + BWI76_RS01940 0.14 +1.0
395,044 + BWI76_RS01940 0.23 -0.4
395,321 + +1.0
395,347 - -0.3
395,442 + +0.1
395,529 - -0.7

Or see this region's nucleotide sequence