Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00370

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00365 and BWI76_RS00370 are separated by 583 nucleotidesBWI76_RS00370 and BWI76_RS00375 are separated by 3 nucleotidesBWI76_RS00375 and BWI76_RS00380 are separated by 2 nucleotides BWI76_RS00365: BWI76_RS00365 - YihA family ribosome biogenesis GTP-binding protein, at 77,335 to 77,967 _RS00365 BWI76_RS00370: BWI76_RS00370 - GTPase-activating protein, at 78,551 to 79,057 _RS00370 BWI76_RS00375: BWI76_RS00375 - hypothetical protein, at 79,061 to 79,243 _RS00375 BWI76_RS00380: BWI76_RS00380 - oxygen-independent coproporphyrinogen III oxidase, at 79,246 to 80,619 _RS00380 Position (kb) 78 79 80Strain fitness (log2 ratio) -1 0 1 2 3at 78.224 kb on + strandat 78.224 kb on + strandat 78.268 kb on - strandat 78.290 kb on + strandat 78.369 kb on - strandat 78.391 kb on - strandat 78.532 kb on + strandat 78.572 kb on - strandat 78.835 kb on - strand, within BWI76_RS00370at 78.885 kb on + strandat 78.885 kb on - strand, within BWI76_RS00370at 78.912 kb on + strand, within BWI76_RS00370at 78.912 kb on + strand, within BWI76_RS00370at 78.922 kb on + strand, within BWI76_RS00370at 78.930 kb on - strand, within BWI76_RS00370at 78.987 kb on - strand, within BWI76_RS00370at 79.102 kb on + strand, within BWI76_RS00375at 79.110 kb on - strand, within BWI76_RS00375at 79.110 kb on - strand, within BWI76_RS00375at 79.234 kb on + strandat 79.250 kb on - strandat 79.270 kb on + strandat 79.271 kb on + strandat 79.273 kb on - strandat 79.308 kb on + strandat 79.316 kb on - strandat 79.316 kb on - strandat 79.316 kb on - strandat 79.482 kb on + strand, within BWI76_RS00380at 79.482 kb on + strand, within BWI76_RS00380at 79.482 kb on + strand, within BWI76_RS00380at 79.482 kb on + strand, within BWI76_RS00380at 79.490 kb on - strand, within BWI76_RS00380at 79.490 kb on - strand, within BWI76_RS00380at 79.490 kb on - strand, within BWI76_RS00380at 79.490 kb on - strandat 79.583 kb on - strand, within BWI76_RS00380at 79.628 kb on - strand, within BWI76_RS00380at 79.657 kb on - strand, within BWI76_RS00380at 79.754 kb on - strand, within BWI76_RS00380at 79.754 kb on - strand, within BWI76_RS00380at 79.756 kb on - strand, within BWI76_RS00380at 79.811 kb on - strand, within BWI76_RS00380at 79.850 kb on + strand, within BWI76_RS00380at 79.858 kb on - strand, within BWI76_RS00380at 79.938 kb on + strand, within BWI76_RS00380at 79.946 kb on - strand, within BWI76_RS00380at 80.038 kb on - strand, within BWI76_RS00380

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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78,224 + +0.2
78,224 + +0.1
78,268 - +0.4
78,290 + +1.7
78,369 - -0.7
78,391 - -0.7
78,532 + -0.7
78,572 - +0.3
78,835 - BWI76_RS00370 0.56 -0.9
78,885 + +2.1
78,885 - BWI76_RS00370 0.66 +0.2
78,912 + BWI76_RS00370 0.71 +1.4
78,912 + BWI76_RS00370 0.71 +2.1
78,922 + BWI76_RS00370 0.73 +0.6
78,930 - BWI76_RS00370 0.75 +3.7
78,987 - BWI76_RS00370 0.86 +0.4
79,102 + BWI76_RS00375 0.22 +0.3
79,110 - BWI76_RS00375 0.27 +0.2
79,110 - BWI76_RS00375 0.27 +0.6
79,234 + +0.4
79,250 - +0.1
79,270 + +3.4
79,271 + +0.2
79,273 - +0.4
79,308 + +0.5
79,316 - -0.0
79,316 - +1.1
79,316 - -0.5
79,482 + BWI76_RS00380 0.17 -0.1
79,482 + BWI76_RS00380 0.17 +0.8
79,482 + BWI76_RS00380 0.17 +0.6
79,482 + BWI76_RS00380 0.17 +1.1
79,490 - BWI76_RS00380 0.18 +0.4
79,490 - BWI76_RS00380 0.18 +0.3
79,490 - BWI76_RS00380 0.18 -0.6
79,490 - -1.5
79,583 - BWI76_RS00380 0.25 -0.1
79,628 - BWI76_RS00380 0.28 +0.3
79,657 - BWI76_RS00380 0.30 -0.5
79,754 - BWI76_RS00380 0.37 -0.2
79,754 - BWI76_RS00380 0.37 -0.3
79,756 - BWI76_RS00380 0.37 -0.0
79,811 - BWI76_RS00380 0.41 +0.0
79,850 + BWI76_RS00380 0.44 -0.2
79,858 - BWI76_RS00380 0.45 +0.2
79,938 + BWI76_RS00380 0.50 -0.2
79,946 - BWI76_RS00380 0.51 +0.2
80,038 - BWI76_RS00380 0.58 +0.3

Or see this region's nucleotide sequence