Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00240

Experiment: 2-Deoxyadenosine 4 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00230 and BWI76_RS00235 are separated by 196 nucleotidesBWI76_RS00235 and BWI76_RS00240 overlap by 22 nucleotidesBWI76_RS00240 and BWI76_RS00245 are separated by 91 nucleotidesBWI76_RS00245 and BWI76_RS00250 are separated by 152 nucleotides BWI76_RS00230: BWI76_RS00230 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 46,681 to 48,570 _RS00230 BWI76_RS00235: BWI76_RS00235 - hypothetical protein, at 48,767 to 48,973 _RS00235 BWI76_RS00240: BWI76_RS00240 - FMN-binding protein MioC, at 48,952 to 49,392 _RS00240 BWI76_RS00245: BWI76_RS00245 - transcriptional regulator AsnC, at 49,484 to 49,942 _RS00245 BWI76_RS00250: BWI76_RS00250 - asparagine synthetase A, at 50,095 to 51,087 _RS00250 Position (kb) 48 49 50Strain fitness (log2 ratio) -1 0 1 2at 48.582 kb on - strandat 48.592 kb on - strandat 49.073 kb on + strand, within BWI76_RS00240at 49.073 kb on + strand, within BWI76_RS00240at 49.102 kb on + strand, within BWI76_RS00240at 49.227 kb on - strand, within BWI76_RS00240at 49.287 kb on + strandat 49.310 kb on + strandat 49.354 kb on + strandat 49.357 kb on - strandat 49.362 kb on - strandat 49.367 kb on - strandat 49.384 kb on - strandat 49.385 kb on - strandat 49.409 kb on + strandat 49.417 kb on - strandat 49.417 kb on - strandat 49.417 kb on - strandat 49.422 kb on - strandat 49.431 kb on - strandat 49.431 kb on - strandat 49.576 kb on + strand, within BWI76_RS00245at 49.641 kb on + strand, within BWI76_RS00245at 49.666 kb on - strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.677 kb on + strand, within BWI76_RS00245at 49.682 kb on + strand, within BWI76_RS00245at 49.685 kb on - strand, within BWI76_RS00245at 49.687 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.743 kb on + strand, within BWI76_RS00245at 49.756 kb on + strand, within BWI76_RS00245at 49.764 kb on - strand, within BWI76_RS00245at 49.766 kb on + strand, within BWI76_RS00245at 49.822 kb on + strand, within BWI76_RS00245at 49.830 kb on + strand, within BWI76_RS00245at 49.830 kb on - strand, within BWI76_RS00245at 49.830 kb on - strand, within BWI76_RS00245at 49.845 kb on - strand, within BWI76_RS00245at 49.861 kb on + strand, within BWI76_RS00245at 49.869 kb on - strand, within BWI76_RS00245at 49.895 kb on + strand, within BWI76_RS00245at 49.896 kb on + strand, within BWI76_RS00245at 49.897 kb on + strandat 49.903 kb on - strandat 50.111 kb on + strandat 50.158 kb on - strandat 50.227 kb on + strand, within BWI76_RS00250at 50.237 kb on - strand, within BWI76_RS00250at 50.300 kb on - strand, within BWI76_RS00250at 50.335 kb on - strand, within BWI76_RS00250at 50.346 kb on + strand, within BWI76_RS00250at 50.367 kb on + strand, within BWI76_RS00250at 50.387 kb on - strand, within BWI76_RS00250

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 4 mM (C)
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48,582 - -0.9
48,592 - -0.8
49,073 + BWI76_RS00240 0.27 +0.4
49,073 + BWI76_RS00240 0.27 +0.7
49,102 + BWI76_RS00240 0.34 -0.5
49,227 - BWI76_RS00240 0.62 +1.8
49,287 + +1.1
49,310 + -0.5
49,354 + +0.0
49,357 - -0.3
49,362 - +0.1
49,367 - +2.4
49,384 - +0.7
49,385 - +0.7
49,409 + +1.0
49,417 - +0.5
49,417 - +0.1
49,417 - -0.0
49,422 - +0.6
49,431 - -0.5
49,431 - +0.4
49,576 + BWI76_RS00245 0.20 -0.1
49,641 + BWI76_RS00245 0.34 +0.4
49,666 - BWI76_RS00245 0.40 +0.3
49,677 + BWI76_RS00245 0.42 -0.3
49,677 + BWI76_RS00245 0.42 -0.1
49,677 + BWI76_RS00245 0.42 +0.2
49,677 + BWI76_RS00245 0.42 +0.2
49,677 + BWI76_RS00245 0.42 -0.8
49,682 + BWI76_RS00245 0.43 +0.0
49,685 - BWI76_RS00245 0.44 -1.5
49,687 + BWI76_RS00245 0.44 -0.4
49,743 + BWI76_RS00245 0.56 +0.4
49,743 + BWI76_RS00245 0.56 -1.1
49,743 + BWI76_RS00245 0.56 +0.0
49,756 + BWI76_RS00245 0.59 +0.0
49,764 - BWI76_RS00245 0.61 -0.5
49,766 + BWI76_RS00245 0.61 +0.1
49,822 + BWI76_RS00245 0.74 -1.0
49,830 + BWI76_RS00245 0.75 +0.3
49,830 - BWI76_RS00245 0.75 +1.1
49,830 - BWI76_RS00245 0.75 -1.5
49,845 - BWI76_RS00245 0.79 +0.8
49,861 + BWI76_RS00245 0.82 -0.3
49,869 - BWI76_RS00245 0.84 -0.0
49,895 + BWI76_RS00245 0.90 +1.3
49,896 + BWI76_RS00245 0.90 +1.5
49,897 + -0.2
49,903 - +0.5
50,111 + -0.1
50,158 - +0.3
50,227 + BWI76_RS00250 0.13 +1.0
50,237 - BWI76_RS00250 0.14 +0.2
50,300 - BWI76_RS00250 0.21 +0.2
50,335 - BWI76_RS00250 0.24 +0.5
50,346 + BWI76_RS00250 0.25 -0.1
50,367 + BWI76_RS00250 0.27 +1.1
50,387 - BWI76_RS00250 0.29 -0.9

Or see this region's nucleotide sequence