Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2825 and Psyr_2826 are separated by 7 nucleotides Psyr_2826 and Psyr_2827 are separated by 85 nucleotides Psyr_2827 and Psyr_2828 are separated by 44 nucleotides
Psyr_2825: Psyr_2825 - DNA methylase N-4/N-6, at 3,399,004 to 3,400,125
_2825
Psyr_2826: Psyr_2826 - hypothetical protein, at 3,400,133 to 3,400,780
_2826
Psyr_2827: Psyr_2827 - phage-related protein, at 3,400,866 to 3,401,723
_2827
Psyr_2828: Psyr_2828 - C-5 cytosine-specific DNA methylase, at 3,401,768 to 3,403,822
_2828
Position (kb)
3400
3401
3402 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3400.030 kb on + strand at 3400.207 kb on + strand, within Psyr_2826 at 3400.208 kb on - strand, within Psyr_2826 at 3400.208 kb on - strand, within Psyr_2826 at 3400.211 kb on + strand, within Psyr_2826 at 3400.211 kb on + strand, within Psyr_2826 at 3400.272 kb on - strand, within Psyr_2826 at 3400.374 kb on + strand, within Psyr_2826 at 3400.424 kb on + strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.480 kb on - strand, within Psyr_2826 at 3400.484 kb on + strand, within Psyr_2826 at 3400.484 kb on + strand, within Psyr_2826 at 3400.493 kb on - strand, within Psyr_2826 at 3400.804 kb on - strand at 3400.820 kb on + strand at 3400.820 kb on + strand at 3400.820 kb on + strand at 3400.821 kb on - strand at 3400.821 kb on - strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.929 kb on + strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3400.930 kb on - strand at 3401.063 kb on + strand, within Psyr_2827 at 3401.064 kb on - strand, within Psyr_2827 at 3401.064 kb on - strand, within Psyr_2827 at 3401.113 kb on - strand, within Psyr_2827 at 3401.169 kb on + strand, within Psyr_2827 at 3401.169 kb on + strand, within Psyr_2827 at 3401.170 kb on - strand, within Psyr_2827 at 3401.232 kb on + strand, within Psyr_2827 at 3401.232 kb on + strand, within Psyr_2827 at 3401.233 kb on - strand, within Psyr_2827 at 3401.233 kb on - strand, within Psyr_2827 at 3401.446 kb on + strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.518 kb on - strand, within Psyr_2827 at 3401.637 kb on + strand, within Psyr_2827 at 3401.637 kb on + strand, within Psyr_2827 at 3401.638 kb on - strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.767 kb on + strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.768 kb on - strand at 3401.804 kb on - strand at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.001 kb on - strand, within Psyr_2828 at 3402.189 kb on + strand, within Psyr_2828 at 3402.190 kb on - strand, within Psyr_2828 at 3402.381 kb on - strand, within Psyr_2828 at 3402.552 kb on + strand, within Psyr_2828 at 3402.552 kb on + strand, within Psyr_2828 at 3402.553 kb on - strand, within Psyr_2828
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 3,400,030 + -0.8 3,400,207 + Psyr_2826 0.11 -1.8 3,400,208 - Psyr_2826 0.12 -0.1 3,400,208 - Psyr_2826 0.12 -0.8 3,400,211 + Psyr_2826 0.12 -1.1 3,400,211 + Psyr_2826 0.12 -0.8 3,400,272 - Psyr_2826 0.21 +1.6 3,400,374 + Psyr_2826 0.37 -1.5 3,400,424 + Psyr_2826 0.45 +0.6 3,400,480 - Psyr_2826 0.54 -1.4 3,400,480 - Psyr_2826 0.54 -0.7 3,400,480 - Psyr_2826 0.54 +1.8 3,400,484 + Psyr_2826 0.54 -0.4 3,400,484 + Psyr_2826 0.54 -1.4 3,400,493 - Psyr_2826 0.56 +0.4 3,400,804 - +0.2 3,400,820 + -0.2 3,400,820 + +0.8 3,400,820 + -0.7 3,400,821 - -1.4 3,400,821 - +0.4 3,400,929 + -1.4 3,400,929 + +0.3 3,400,929 + -0.3 3,400,929 + -0.8 3,400,929 + +0.4 3,400,929 + +1.2 3,400,929 + -0.7 3,400,930 - +1.2 3,400,930 - -0.8 3,400,930 - +1.9 3,400,930 - +0.6 3,400,930 - +1.2 3,400,930 - -0.4 3,400,930 - +1.1 3,401,063 + Psyr_2827 0.23 -0.7 3,401,064 - Psyr_2827 0.23 -1.2 3,401,064 - Psyr_2827 0.23 -0.0 3,401,113 - Psyr_2827 0.29 -0.5 3,401,169 + Psyr_2827 0.35 -1.1 3,401,169 + Psyr_2827 0.35 -1.4 3,401,170 - Psyr_2827 0.35 -0.8 3,401,232 + Psyr_2827 0.43 +3.8 3,401,232 + Psyr_2827 0.43 +0.1 3,401,233 - Psyr_2827 0.43 -0.8 3,401,233 - Psyr_2827 0.43 -1.3 3,401,446 + Psyr_2827 0.68 +0.9 3,401,518 - Psyr_2827 0.76 -0.6 3,401,518 - Psyr_2827 0.76 +0.2 3,401,518 - Psyr_2827 0.76 -2.5 3,401,637 + Psyr_2827 0.90 -1.1 3,401,637 + Psyr_2827 0.90 +0.6 3,401,638 - +0.9 3,401,767 + +1.0 3,401,767 + -0.5 3,401,767 + -0.7 3,401,767 + +2.2 3,401,767 + +0.7 3,401,767 + -2.5 3,401,767 + -0.2 3,401,767 + +1.4 3,401,767 + +1.4 3,401,767 + -0.2 3,401,768 - -1.2 3,401,768 - +0.2 3,401,768 - -0.7 3,401,768 - +1.0 3,401,768 - +0.8 3,401,768 - -0.3 3,401,768 - +0.0 3,401,768 - -0.6 3,401,768 - +1.6 3,401,768 - +0.5 3,401,768 - +1.2 3,401,804 - -0.1 3,402,001 - Psyr_2828 0.11 -1.9 3,402,001 - Psyr_2828 0.11 +0.2 3,402,001 - Psyr_2828 0.11 -3.1 3,402,001 - Psyr_2828 0.11 +0.1 3,402,189 + Psyr_2828 0.20 +0.4 3,402,190 - Psyr_2828 0.21 -2.4 3,402,381 - Psyr_2828 0.30 +0.7 3,402,552 + Psyr_2828 0.38 +0.2 3,402,552 + Psyr_2828 0.38 +0.8 3,402,553 - Psyr_2828 0.38 +0.4
Or see this region's nucleotide sequence