Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2460 and Psyr_2461 are separated by 164 nucleotides Psyr_2461 and Psyr_2462 overlap by 17 nucleotides Psyr_2462 and Psyr_2463 are separated by 554 nucleotides
Psyr_2460: Psyr_2460 - inner membrane peptidase, Serine peptidase, MEROPS family S49, at 2,847,916 to 2,848,938
_2460
Psyr_2461: Psyr_2461 - Uncharacterized conserved protein UCP030820, at 2,849,103 to 2,849,597
_2461
Psyr_2462: Psyr_2462 - Nitrite/sulfite reductase, hemoprotein beta-component, ferrodoxin-like:Nitrite and sulphite reductase 4Fe-4S region, at 2,849,581 to 2,851,239
_2462
Psyr_2463: Psyr_2463 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 2,851,794 to 2,852,894
_2463
Position (kb)
2849
2850
2851
2852 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2848.721 kb on + strand, within Psyr_2460 at 2848.721 kb on + strand, within Psyr_2460 at 2848.721 kb on + strand, within Psyr_2460 at 2848.722 kb on - strand, within Psyr_2460 at 2848.722 kb on - strand, within Psyr_2460 at 2848.828 kb on + strand, within Psyr_2460 at 2848.828 kb on + strand, within Psyr_2460 at 2848.829 kb on - strand, within Psyr_2460 at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.939 kb on + strand at 2848.940 kb on - strand at 2848.940 kb on - strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.945 kb on + strand at 2848.946 kb on - strand at 2848.946 kb on - strand at 2849.149 kb on + strand at 2849.188 kb on + strand, within Psyr_2461 at 2849.242 kb on + strand, within Psyr_2461 at 2849.242 kb on + strand, within Psyr_2461 at 2849.243 kb on - strand, within Psyr_2461 at 2849.243 kb on - strand, within Psyr_2461 at 2849.294 kb on - strand, within Psyr_2461 at 2849.597 kb on + strand at 2849.628 kb on + strand at 2849.629 kb on - strand at 2849.636 kb on + strand at 2849.637 kb on - strand at 2849.637 kb on - strand at 2849.679 kb on - strand at 2849.792 kb on + strand, within Psyr_2462 at 2849.877 kb on + strand, within Psyr_2462 at 2849.878 kb on - strand, within Psyr_2462 at 2849.880 kb on + strand, within Psyr_2462 at 2850.240 kb on - strand, within Psyr_2462 at 2850.347 kb on + strand, within Psyr_2462 at 2850.347 kb on + strand, within Psyr_2462 at 2850.348 kb on - strand, within Psyr_2462 at 2850.500 kb on + strand, within Psyr_2462 at 2850.500 kb on + strand, within Psyr_2462 at 2850.501 kb on - strand, within Psyr_2462 at 2850.501 kb on - strand, within Psyr_2462 at 2850.501 kb on - strand, within Psyr_2462 at 2850.501 kb on - strand at 2850.531 kb on - strand, within Psyr_2462 at 2850.758 kb on + strand, within Psyr_2462 at 2850.895 kb on - strand, within Psyr_2462 at 2850.967 kb on + strand, within Psyr_2462 at 2850.968 kb on - strand, within Psyr_2462 at 2851.003 kb on - strand, within Psyr_2462 at 2851.103 kb on + strand at 2851.103 kb on + strand at 2851.103 kb on + strand at 2851.103 kb on + strand at 2851.103 kb on + strand at 2851.103 kb on + strand at 2851.164 kb on - strand at 2851.231 kb on + strand at 2851.231 kb on + strand at 2851.232 kb on - strand at 2851.232 kb on - strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.235 kb on + strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.236 kb on - strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.239 kb on + strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.240 kb on - strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.241 kb on + strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.242 kb on - strand at 2851.461 kb on + strand at 2851.495 kb on - strand at 2851.495 kb on - strand at 2851.495 kb on - strand at 2851.638 kb on + strand at 2851.638 kb on + strand at 2851.639 kb on - strand at 2851.660 kb on - strand at 2851.794 kb on - strand at 2851.806 kb on + strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.807 kb on - strand at 2851.822 kb on + strand at 2851.823 kb on - strand at 2851.823 kb on - strand at 2851.966 kb on + strand, within Psyr_2463 at 2851.966 kb on + strand, within Psyr_2463 at 2851.966 kb on + strand, within Psyr_2463 at 2851.967 kb on - strand, within Psyr_2463 at 2851.971 kb on + strand, within Psyr_2463 at 2851.971 kb on + strand, within Psyr_2463 at 2851.971 kb on + strand, within Psyr_2463 at 2851.972 kb on - strand, within Psyr_2463 at 2851.972 kb on - strand, within Psyr_2463 at 2851.972 kb on - strand, within Psyr_2463 at 2851.972 kb on - strand, within Psyr_2463 at 2852.044 kb on - strand, within Psyr_2463 at 2852.044 kb on - strand, within Psyr_2463 at 2852.090 kb on + strand, within Psyr_2463 at 2852.090 kb on + strand, within Psyr_2463 at 2852.090 kb on + strand, within Psyr_2463 at 2852.090 kb on + strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463 at 2852.091 kb on - strand, within Psyr_2463
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 2,848,721 + Psyr_2460 0.79 -0.2 2,848,721 + Psyr_2460 0.79 +1.1 2,848,721 + Psyr_2460 0.79 -0.1 2,848,722 - Psyr_2460 0.79 +1.2 2,848,722 - Psyr_2460 0.79 -1.1 2,848,828 + Psyr_2460 0.89 -2.1 2,848,828 + Psyr_2460 0.89 -1.4 2,848,829 - Psyr_2460 0.89 -2.9 2,848,939 + -0.1 2,848,939 + +1.0 2,848,939 + -0.0 2,848,939 + +0.5 2,848,939 + -0.3 2,848,939 + +0.9 2,848,940 - +0.8 2,848,940 - +2.2 2,848,945 + -1.9 2,848,945 + -1.0 2,848,945 + -0.4 2,848,945 + +2.6 2,848,945 + -0.9 2,848,946 - -1.2 2,848,946 - -0.4 2,849,149 + -1.1 2,849,188 + Psyr_2461 0.17 +1.6 2,849,242 + Psyr_2461 0.28 +0.1 2,849,242 + Psyr_2461 0.28 -1.8 2,849,243 - Psyr_2461 0.28 +0.5 2,849,243 - Psyr_2461 0.28 +0.1 2,849,294 - Psyr_2461 0.39 +1.2 2,849,597 + -2.3 2,849,628 + -0.1 2,849,629 - -1.6 2,849,636 + -2.2 2,849,637 - +0.2 2,849,637 - -0.4 2,849,679 - -0.3 2,849,792 + Psyr_2462 0.13 -1.1 2,849,877 + Psyr_2462 0.18 -1.1 2,849,878 - Psyr_2462 0.18 -0.8 2,849,880 + Psyr_2462 0.18 -0.2 2,850,240 - Psyr_2462 0.40 -1.0 2,850,347 + Psyr_2462 0.46 -0.9 2,850,347 + Psyr_2462 0.46 +1.5 2,850,348 - Psyr_2462 0.46 -0.9 2,850,500 + Psyr_2462 0.55 -2.1 2,850,500 + Psyr_2462 0.55 -1.6 2,850,501 - Psyr_2462 0.55 +0.1 2,850,501 - Psyr_2462 0.55 -1.1 2,850,501 - Psyr_2462 0.55 +0.4 2,850,501 - -0.3 2,850,531 - Psyr_2462 0.57 -1.2 2,850,758 + Psyr_2462 0.71 -0.6 2,850,895 - Psyr_2462 0.79 -1.1 2,850,967 + Psyr_2462 0.84 -2.3 2,850,968 - Psyr_2462 0.84 -1.1 2,851,003 - Psyr_2462 0.86 -0.8 2,851,103 + -2.0 2,851,103 + +0.9 2,851,103 + -0.0 2,851,103 + -1.1 2,851,103 + -1.8 2,851,103 + -0.5 2,851,164 - -0.3 2,851,231 + -0.8 2,851,231 + -2.1 2,851,232 - -2.8 2,851,232 - +0.0 2,851,235 + -1.9 2,851,235 + +1.2 2,851,235 + +1.6 2,851,235 + -1.5 2,851,235 + +0.7 2,851,235 + +1.8 2,851,235 + -0.7 2,851,235 + -1.1 2,851,235 + -1.9 2,851,235 + -0.1 2,851,235 + -1.2 2,851,235 + +0.2 2,851,235 + -0.2 2,851,235 + +0.5 2,851,235 + -0.1 2,851,235 + -0.8 2,851,236 - -0.3 2,851,236 - -2.7 2,851,236 - -0.3 2,851,236 - -0.6 2,851,236 - +0.2 2,851,236 - -0.6 2,851,236 - +1.0 2,851,236 - -0.2 2,851,236 - -1.3 2,851,236 - -1.2 2,851,236 - -0.6 2,851,236 - +0.0 2,851,236 - -0.8 2,851,239 + -0.2 2,851,239 + -2.1 2,851,239 + +1.2 2,851,239 + +0.5 2,851,239 + -1.1 2,851,239 + +1.6 2,851,239 + +1.8 2,851,239 + -2.9 2,851,239 + -1.3 2,851,239 + -0.5 2,851,240 - -1.3 2,851,240 - +0.2 2,851,240 - -1.7 2,851,240 - +0.1 2,851,240 - -0.8 2,851,240 - -0.7 2,851,240 - -1.3 2,851,240 - -1.1 2,851,240 - -0.8 2,851,240 - -0.0 2,851,240 - -1.8 2,851,240 - -1.1 2,851,240 - -0.5 2,851,240 - +0.4 2,851,241 + +0.2 2,851,241 + -1.0 2,851,241 + +0.0 2,851,241 + -1.3 2,851,241 + +0.5 2,851,241 + +0.2 2,851,241 + -0.9 2,851,241 + -0.6 2,851,241 + -1.1 2,851,241 + -1.1 2,851,241 + -0.5 2,851,241 + +0.0 2,851,241 + +1.2 2,851,241 + +0.9 2,851,242 - -0.3 2,851,242 - -0.5 2,851,242 - -0.2 2,851,242 - +1.2 2,851,242 - -0.8 2,851,242 - +2.8 2,851,242 - +3.6 2,851,242 - -1.2 2,851,242 - -0.9 2,851,242 - -0.9 2,851,461 + -0.8 2,851,495 - +1.7 2,851,495 - +0.1 2,851,495 - -0.9 2,851,638 + -0.6 2,851,638 + -0.3 2,851,639 - +0.4 2,851,660 - -0.6 2,851,794 - +0.2 2,851,806 + -1.6 2,851,807 - +1.2 2,851,807 - -0.3 2,851,807 - -0.1 2,851,807 - -2.1 2,851,807 - -0.8 2,851,807 - +0.2 2,851,807 - +0.2 2,851,807 - -0.3 2,851,807 - +0.2 2,851,822 + +0.1 2,851,823 - -1.0 2,851,823 - +0.5 2,851,966 + Psyr_2463 0.16 -0.7 2,851,966 + Psyr_2463 0.16 -1.5 2,851,966 + Psyr_2463 0.16 +0.2 2,851,967 - Psyr_2463 0.16 +2.2 2,851,971 + Psyr_2463 0.16 -0.0 2,851,971 + Psyr_2463 0.16 +0.4 2,851,971 + Psyr_2463 0.16 -1.6 2,851,972 - Psyr_2463 0.16 -1.1 2,851,972 - Psyr_2463 0.16 +0.2 2,851,972 - Psyr_2463 0.16 +1.7 2,851,972 - Psyr_2463 0.16 -1.6 2,852,044 - Psyr_2463 0.23 -0.4 2,852,044 - Psyr_2463 0.23 -1.2 2,852,090 + Psyr_2463 0.27 +0.5 2,852,090 + Psyr_2463 0.27 -1.9 2,852,090 + Psyr_2463 0.27 +0.5 2,852,090 + Psyr_2463 0.27 +1.1 2,852,091 - Psyr_2463 0.27 -1.5 2,852,091 - Psyr_2463 0.27 +2.1 2,852,091 - Psyr_2463 0.27 +0.6 2,852,091 - Psyr_2463 0.27 -0.8 2,852,091 - Psyr_2463 0.27 -1.1 2,852,091 - Psyr_2463 0.27 -1.2 2,852,091 - Psyr_2463 0.27 +0.1
Or see this region's nucleotide sequence