Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2345 and Psyr_2346 overlap by 4 nucleotides Psyr_2346 and Psyr_2347 are separated by 271 nucleotides
Psyr_2345: Psyr_2345 - ea59 protein, at 2,721,143 to 2,722,693
_2345
Psyr_2346: Psyr_2346 - conserved hypothetical protein, at 2,722,690 to 2,723,565
_2346
Psyr_2347: Psyr_2347 - hypothetical protein, at 2,723,837 to 2,724,484
_2347
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2721.767 kb on - strand, within Psyr_2345 at 2721.976 kb on + strand, within Psyr_2345 at 2722.039 kb on + strand, within Psyr_2345 at 2722.172 kb on - strand, within Psyr_2345 at 2722.272 kb on + strand, within Psyr_2345 at 2722.302 kb on + strand, within Psyr_2345 at 2722.347 kb on + strand, within Psyr_2345 at 2722.350 kb on + strand, within Psyr_2345 at 2722.350 kb on + strand, within Psyr_2345 at 2722.351 kb on - strand, within Psyr_2345 at 2722.351 kb on - strand, within Psyr_2345 at 2722.444 kb on - strand, within Psyr_2345 at 2722.447 kb on + strand, within Psyr_2345 at 2722.447 kb on + strand, within Psyr_2345 at 2722.597 kb on + strand at 2722.597 kb on + strand at 2722.598 kb on - strand at 2722.657 kb on + strand at 2722.687 kb on + strand at 2722.687 kb on + strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.688 kb on - strand at 2722.689 kb on + strand at 2722.689 kb on + strand at 2722.689 kb on + strand at 2722.690 kb on - strand at 2722.690 kb on - strand at 2722.690 kb on - strand at 2722.694 kb on + strand at 2722.694 kb on + strand at 2722.694 kb on + strand at 2722.737 kb on + strand at 2722.738 kb on - strand at 2722.755 kb on - strand at 2722.769 kb on + strand at 2722.803 kb on - strand, within Psyr_2346 at 2722.822 kb on + strand, within Psyr_2346 at 2722.854 kb on + strand, within Psyr_2346 at 2722.854 kb on + strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.923 kb on - strand, within Psyr_2346 at 2722.990 kb on - strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.070 kb on + strand, within Psyr_2346 at 2723.075 kb on - strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.121 kb on + strand, within Psyr_2346 at 2723.221 kb on + strand, within Psyr_2346 at 2723.222 kb on - strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.389 kb on + strand, within Psyr_2346 at 2723.390 kb on - strand, within Psyr_2346 at 2723.396 kb on + strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.401 kb on - strand, within Psyr_2346 at 2723.442 kb on - strand, within Psyr_2346 at 2723.445 kb on + strand, within Psyr_2346 at 2723.446 kb on - strand, within Psyr_2346 at 2723.601 kb on + strand at 2723.602 kb on - strand at 2723.715 kb on + strand at 2723.761 kb on + strand at 2723.961 kb on - strand, within Psyr_2347 at 2723.970 kb on - strand, within Psyr_2347 at 2724.061 kb on - strand, within Psyr_2347 at 2724.128 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.258 kb on - strand, within Psyr_2347 at 2724.311 kb on + strand at 2724.471 kb on + strand at 2724.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 2,721,767 - Psyr_2345 0.40 -1.4 2,721,976 + Psyr_2345 0.54 -0.2 2,722,039 + Psyr_2345 0.58 -3.9 2,722,172 - Psyr_2345 0.66 -0.4 2,722,272 + Psyr_2345 0.73 -3.2 2,722,302 + Psyr_2345 0.75 +0.2 2,722,347 + Psyr_2345 0.78 +1.6 2,722,350 + Psyr_2345 0.78 -0.9 2,722,350 + Psyr_2345 0.78 -2.8 2,722,351 - Psyr_2345 0.78 -2.6 2,722,351 - Psyr_2345 0.78 +0.6 2,722,444 - Psyr_2345 0.84 +1.2 2,722,447 + Psyr_2345 0.84 +0.4 2,722,447 + Psyr_2345 0.84 +0.2 2,722,597 + -1.5 2,722,597 + -0.1 2,722,598 - -2.1 2,722,657 + -1.6 2,722,687 + -1.1 2,722,687 + -0.2 2,722,688 - -2.1 2,722,688 - -0.2 2,722,688 - -2.2 2,722,688 - -2.1 2,722,689 + -0.1 2,722,689 + -1.4 2,722,689 + +0.4 2,722,690 - -2.7 2,722,690 - -2.6 2,722,690 - -0.8 2,722,694 + +0.2 2,722,694 + -0.4 2,722,694 + -0.4 2,722,737 + +0.8 2,722,738 - -2.4 2,722,755 - -1.1 2,722,769 + -0.9 2,722,803 - Psyr_2346 0.13 +0.2 2,722,822 + Psyr_2346 0.15 -0.6 2,722,854 + Psyr_2346 0.19 -1.6 2,722,854 + Psyr_2346 0.19 +0.2 2,722,923 - Psyr_2346 0.27 -0.1 2,722,923 - Psyr_2346 0.27 -0.9 2,722,990 - Psyr_2346 0.34 -1.6 2,723,070 + Psyr_2346 0.43 -2.4 2,723,070 + Psyr_2346 0.43 -0.4 2,723,075 - Psyr_2346 0.44 -0.4 2,723,121 + Psyr_2346 0.49 +0.8 2,723,121 + Psyr_2346 0.49 -1.9 2,723,221 + Psyr_2346 0.61 -1.6 2,723,222 - Psyr_2346 0.61 -1.1 2,723,389 + Psyr_2346 0.80 -0.1 2,723,389 + Psyr_2346 0.80 -2.0 2,723,390 - Psyr_2346 0.80 -0.1 2,723,396 + Psyr_2346 0.81 -0.8 2,723,401 - Psyr_2346 0.81 -2.1 2,723,401 - Psyr_2346 0.81 -1.4 2,723,401 - Psyr_2346 0.81 -0.4 2,723,442 - Psyr_2346 0.86 -0.7 2,723,445 + Psyr_2346 0.86 -0.3 2,723,446 - Psyr_2346 0.86 -1.1 2,723,601 + -1.0 2,723,602 - -0.4 2,723,715 + +0.5 2,723,761 + +1.2 2,723,961 - Psyr_2347 0.19 -2.1 2,723,970 - Psyr_2347 0.21 +0.6 2,724,061 - Psyr_2347 0.35 -3.0 2,724,128 - Psyr_2347 0.45 -2.4 2,724,258 - Psyr_2347 0.65 -1.2 2,724,258 - Psyr_2347 0.65 -1.4 2,724,311 + +1.2 2,724,471 + +1.2 2,724,509 - -0.2
Or see this region's nucleotide sequence