Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2257 and Psyr_2258 overlap by 1 nucleotides Psyr_2258 and Psyr_2259 overlap by 10 nucleotides Psyr_2259 and Psyr_2260 are separated by 9 nucleotides
Psyr_2257: Psyr_2257 - Amidohydrolase, at 2,621,628 to 2,622,773
_2257
Psyr_2258: Psyr_2258 - Guanylate kinase/L-type calcium channel region, at 2,622,773 to 2,623,339
_2258
Psyr_2259: Psyr_2259 - Beta-lactamase-like protein, at 2,623,330 to 2,624,082
_2259
Psyr_2260: Psyr_2260 - Amidase, at 2,624,092 to 2,625,615
_2260
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2622.543 kb on + strand at 2622.676 kb on + strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.677 kb on - strand at 2622.791 kb on + strand at 2622.791 kb on + strand at 2622.791 kb on + strand at 2622.792 kb on - strand at 2622.792 kb on - strand at 2623.267 kb on + strand, within Psyr_2258 at 2623.378 kb on + strand at 2623.552 kb on + strand, within Psyr_2259 at 2623.846 kb on + strand, within Psyr_2259 at 2623.998 kb on + strand, within Psyr_2259 at 2623.999 kb on - strand, within Psyr_2259 at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.048 kb on + strand at 2624.049 kb on - strand at 2624.067 kb on + strand at 2624.068 kb on - strand at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.402 kb on + strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand, within Psyr_2260 at 2624.403 kb on - strand at 2624.472 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.495 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.880 kb on + strand, within Psyr_2260 at 2624.881 kb on - strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.882 kb on + strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.883 kb on - strand, within Psyr_2260 at 2624.965 kb on + strand, within Psyr_2260
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 2,622,543 + -1.1 2,622,676 + +0.6 2,622,677 - -1.8 2,622,677 - -0.8 2,622,677 - -1.6 2,622,677 - -0.2 2,622,677 - +1.0 2,622,677 - +1.5 2,622,791 + +0.9 2,622,791 + -0.6 2,622,791 + -0.7 2,622,792 - -0.1 2,622,792 - +0.7 2,623,267 + Psyr_2258 0.87 -0.1 2,623,378 + +0.1 2,623,552 + Psyr_2259 0.29 +0.9 2,623,846 + Psyr_2259 0.69 -0.9 2,623,998 + Psyr_2259 0.89 -1.2 2,623,999 - Psyr_2259 0.89 -0.2 2,624,048 + -0.1 2,624,048 + -0.0 2,624,048 + +0.5 2,624,048 + +1.0 2,624,049 - +0.5 2,624,067 + -2.3 2,624,068 - +1.1 2,624,402 + Psyr_2260 0.20 -0.2 2,624,402 + Psyr_2260 0.20 +1.0 2,624,402 + Psyr_2260 0.20 +0.5 2,624,403 - Psyr_2260 0.20 -0.1 2,624,403 - Psyr_2260 0.20 +0.2 2,624,403 - Psyr_2260 0.20 -1.1 2,624,403 - +0.2 2,624,472 + Psyr_2260 0.25 +0.8 2,624,495 + Psyr_2260 0.26 -0.2 2,624,495 + Psyr_2260 0.26 -1.1 2,624,880 + Psyr_2260 0.52 -1.8 2,624,880 + Psyr_2260 0.52 -0.5 2,624,881 - Psyr_2260 0.52 +0.4 2,624,882 + Psyr_2260 0.52 +2.0 2,624,882 + Psyr_2260 0.52 +1.1 2,624,882 + Psyr_2260 0.52 +1.2 2,624,882 + Psyr_2260 0.52 +0.3 2,624,883 - Psyr_2260 0.52 +0.9 2,624,883 - Psyr_2260 0.52 -2.0 2,624,965 + Psyr_2260 0.57 +0.2
Or see this region's nucleotide sequence