Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1746 and Psyr_1747 are separated by 91 nucleotides Psyr_1747 and Psyr_1748 are separated by 106 nucleotides Psyr_1748 and Psyr_1749 are separated by 207 nucleotides
Psyr_1746: Psyr_1746 - Trigger factor, at 1,983,381 to 1,984,691
_1746
Psyr_1747: Psyr_1747 - ATP-dependent Clp protease proteolytic subunit ClpP, at 1,984,783 to 1,985,424
_1747
Psyr_1748: Psyr_1748 - ATP-dependent Clp protease ATP-binding subunit ClpX, at 1,985,531 to 1,986,814
_1748
Psyr_1749: Psyr_1749 - ATP-dependent proteinase, Serine peptidase, MEROPS family S16, at 1,987,022 to 1,989,418
_1749
Position (kb)
1985
1986
1987 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1984.532 kb on - strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.542 kb on + strand, within Psyr_1746 at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.570 kb on + strand at 1984.571 kb on - strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.600 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.604 kb on + strand at 1984.647 kb on + strand at 1984.761 kb on + strand at 1984.762 kb on - strand at 1984.799 kb on - strand at 1984.882 kb on + strand, within Psyr_1747 at 1984.891 kb on + strand, within Psyr_1747 at 1984.892 kb on - strand, within Psyr_1747 at 1984.945 kb on + strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1984.946 kb on - strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.017 kb on + strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.018 kb on - strand, within Psyr_1747 at 1985.423 kb on - strand at 1985.423 kb on - strand at 1985.573 kb on + strand at 1985.573 kb on + strand at 1985.574 kb on - strand at 1985.638 kb on + strand at 1985.719 kb on + strand, within Psyr_1748 at 1985.719 kb on + strand, within Psyr_1748 at 1985.720 kb on - strand, within Psyr_1748 at 1985.766 kb on + strand, within Psyr_1748 at 1985.767 kb on - strand, within Psyr_1748 at 1985.810 kb on + strand, within Psyr_1748 at 1985.810 kb on + strand, within Psyr_1748 at 1985.811 kb on - strand, within Psyr_1748 at 1985.819 kb on + strand, within Psyr_1748 at 1985.949 kb on + strand, within Psyr_1748 at 1985.993 kb on + strand, within Psyr_1748 at 1985.994 kb on - strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.054 kb on + strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.055 kb on - strand, within Psyr_1748 at 1986.080 kb on + strand, within Psyr_1748 at 1986.080 kb on + strand, within Psyr_1748 at 1986.259 kb on + strand, within Psyr_1748 at 1986.260 kb on - strand, within Psyr_1748 at 1986.272 kb on - strand, within Psyr_1748 at 1986.501 kb on + strand, within Psyr_1748 at 1986.543 kb on - strand, within Psyr_1748 at 1986.686 kb on + strand at 1986.686 kb on + strand at 1986.686 kb on + strand at 1986.686 kb on + strand at 1986.686 kb on + strand at 1986.770 kb on + strand at 1986.771 kb on - strand at 1986.771 kb on - strand at 1986.771 kb on - strand at 1986.839 kb on + strand at 1986.839 kb on + strand at 1986.839 kb on + strand at 1986.839 kb on + strand at 1986.839 kb on + strand at 1986.839 kb on + strand at 1986.840 kb on - strand at 1986.840 kb on - strand at 1986.842 kb on + strand at 1986.932 kb on + strand at 1986.978 kb on - strand at 1987.074 kb on - strand at 1987.214 kb on + strand at 1987.215 kb on - strand at 1987.239 kb on + strand at 1987.430 kb on + strand, within Psyr_1749 at 1987.431 kb on - strand, within Psyr_1749 at 1987.692 kb on - strand, within Psyr_1749 at 1987.694 kb on + strand, within Psyr_1749 at 1987.694 kb on + strand, within Psyr_1749 at 1987.695 kb on - strand, within Psyr_1749 at 1987.695 kb on - strand, within Psyr_1749
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 1,984,532 - Psyr_1746 0.88 +0.1 1,984,542 + Psyr_1746 0.89 -0.1 1,984,542 + Psyr_1746 0.89 +0.5 1,984,542 + Psyr_1746 0.89 +0.6 1,984,542 + Psyr_1746 0.89 +0.5 1,984,570 + -0.6 1,984,570 + -0.5 1,984,570 + -1.0 1,984,570 + -0.2 1,984,571 - +1.4 1,984,600 + -1.0 1,984,600 + -0.5 1,984,600 + -0.5 1,984,600 + +0.3 1,984,600 + +0.5 1,984,604 + +0.1 1,984,604 + +0.3 1,984,604 + +1.1 1,984,604 + -0.5 1,984,647 + +1.1 1,984,761 + -0.5 1,984,762 - -0.7 1,984,799 - +2.1 1,984,882 + Psyr_1747 0.15 -0.0 1,984,891 + Psyr_1747 0.17 +1.1 1,984,892 - Psyr_1747 0.17 +0.4 1,984,945 + Psyr_1747 0.25 -1.1 1,984,946 - Psyr_1747 0.25 -1.0 1,984,946 - Psyr_1747 0.25 -0.9 1,985,017 + Psyr_1747 0.36 +0.3 1,985,017 + Psyr_1747 0.36 -0.9 1,985,017 + Psyr_1747 0.36 -0.9 1,985,018 - Psyr_1747 0.37 +1.1 1,985,018 - Psyr_1747 0.37 +1.1 1,985,018 - Psyr_1747 0.37 -0.2 1,985,018 - Psyr_1747 0.37 +0.5 1,985,018 - Psyr_1747 0.37 +0.1 1,985,423 - +3.1 1,985,423 - +0.1 1,985,573 + -0.7 1,985,573 + -1.5 1,985,574 - -0.4 1,985,638 + -0.7 1,985,719 + Psyr_1748 0.15 -1.2 1,985,719 + Psyr_1748 0.15 -1.1 1,985,720 - Psyr_1748 0.15 -1.7 1,985,766 + Psyr_1748 0.18 +0.5 1,985,767 - Psyr_1748 0.18 -3.1 1,985,810 + Psyr_1748 0.22 -1.9 1,985,810 + Psyr_1748 0.22 +0.7 1,985,811 - Psyr_1748 0.22 -2.2 1,985,819 + Psyr_1748 0.22 +0.5 1,985,949 + Psyr_1748 0.33 -1.4 1,985,993 + Psyr_1748 0.36 +1.1 1,985,994 - Psyr_1748 0.36 -1.9 1,986,054 + Psyr_1748 0.41 -1.6 1,986,054 + Psyr_1748 0.41 +0.7 1,986,054 + Psyr_1748 0.41 -0.3 1,986,055 - Psyr_1748 0.41 -0.0 1,986,055 - Psyr_1748 0.41 -0.9 1,986,055 - Psyr_1748 0.41 -1.3 1,986,080 + Psyr_1748 0.43 +1.5 1,986,080 + Psyr_1748 0.43 -2.2 1,986,259 + Psyr_1748 0.57 +0.4 1,986,260 - Psyr_1748 0.57 +0.1 1,986,272 - Psyr_1748 0.58 -2.5 1,986,501 + Psyr_1748 0.76 -1.9 1,986,543 - Psyr_1748 0.79 -2.6 1,986,686 + +0.1 1,986,686 + -0.7 1,986,686 + -1.0 1,986,686 + +1.1 1,986,686 + +0.6 1,986,770 + -0.3 1,986,771 - -2.8 1,986,771 - -1.9 1,986,771 - -2.2 1,986,839 + -0.9 1,986,839 + +0.1 1,986,839 + -0.9 1,986,839 + -1.3 1,986,839 + -1.2 1,986,839 + -0.5 1,986,840 - -0.2 1,986,840 - -1.2 1,986,842 + +0.7 1,986,932 + +0.7 1,986,978 - -1.1 1,987,074 - +0.4 1,987,214 + +0.6 1,987,215 - -2.5 1,987,239 + -0.1 1,987,430 + Psyr_1749 0.17 +0.1 1,987,431 - Psyr_1749 0.17 -0.7 1,987,692 - Psyr_1749 0.28 +0.4 1,987,694 + Psyr_1749 0.28 -1.0 1,987,694 + Psyr_1749 0.28 -0.3 1,987,695 - Psyr_1749 0.28 -0.7 1,987,695 - Psyr_1749 0.28 +0.5
Or see this region's nucleotide sequence