Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1665 and Psyr_1666 overlap by 17 nucleotides Psyr_1666 and Psyr_1667 are separated by 118 nucleotides Psyr_1667 and Psyr_1668 are separated by 12 nucleotides
Psyr_1665: Psyr_1665 - Folylpolyglutamate synthetase, at 1,869,795 to 1,871,099
_1665
Psyr_1666: Psyr_1666 - Sporulation related protein, at 1,871,083 to 1,871,736
_1666
Psyr_1667: Psyr_1667 - Colicin V production protein, at 1,871,855 to 1,872,442
_1667
Psyr_1668: Psyr_1668 - amidophosphoribosyltransferase, at 1,872,455 to 1,873,963
_1668
Position (kb)
1871
1872
1873 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.102 kb on + strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.103 kb on - strand at 1871.584 kb on + strand, within Psyr_1666 at 1871.584 kb on + strand, within Psyr_1666 at 1871.584 kb on + strand, within Psyr_1666 at 1871.585 kb on - strand, within Psyr_1666 at 1871.593 kb on + strand, within Psyr_1666 at 1871.593 kb on + strand, within Psyr_1666 at 1871.594 kb on - strand, within Psyr_1666 at 1871.594 kb on - strand, within Psyr_1666 at 1871.703 kb on + strand at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.023 kb on + strand, within Psyr_1667 at 1872.024 kb on - strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.027 kb on + strand, within Psyr_1667 at 1872.028 kb on - strand, within Psyr_1667 at 1872.028 kb on - strand, within Psyr_1667 at 1872.061 kb on + strand, within Psyr_1667 at 1872.114 kb on + strand, within Psyr_1667 at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.397 kb on + strand at 1872.398 kb on - strand at 1872.398 kb on - strand at 1872.436 kb on + strand at 1872.437 kb on - strand at 1872.437 kb on - strand at 1872.506 kb on + strand at 1872.506 kb on + strand at 1872.506 kb on + strand at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.631 kb on + strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.632 kb on - strand, within Psyr_1668 at 1872.653 kb on - strand, within Psyr_1668 at 1872.908 kb on - strand, within Psyr_1668 at 1873.021 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.061 kb on + strand, within Psyr_1668 at 1873.062 kb on - strand, within Psyr_1668 at 1873.062 kb on - strand, within Psyr_1668 at 1873.142 kb on + strand, within Psyr_1668 at 1873.142 kb on + strand, within Psyr_1668 at 1873.143 kb on - strand, within Psyr_1668 at 1873.197 kb on - strand, within Psyr_1668 at 1873.197 kb on - strand, within Psyr_1668 at 1873.224 kb on - strand, within Psyr_1668 at 1873.224 kb on - strand, within Psyr_1668 at 1873.256 kb on - strand, within Psyr_1668 at 1873.265 kb on + strand, within Psyr_1668 at 1873.265 kb on + strand, within Psyr_1668 at 1873.266 kb on - strand, within Psyr_1668 at 1873.436 kb on + strand, within Psyr_1668 at 1873.436 kb on + strand, within Psyr_1668 at 1873.437 kb on - strand, within Psyr_1668 at 1873.437 kb on - strand, within Psyr_1668
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 1,871,102 + -0.1 1,871,102 + -0.8 1,871,102 + -0.0 1,871,102 + -0.6 1,871,102 + +0.1 1,871,102 + -0.1 1,871,102 + -0.3 1,871,103 - -0.5 1,871,103 - -2.0 1,871,103 - -1.9 1,871,103 - -0.4 1,871,103 - +0.4 1,871,103 - -0.6 1,871,103 - -3.1 1,871,103 - +1.9 1,871,103 - -0.7 1,871,103 - +0.1 1,871,584 + Psyr_1666 0.77 +0.9 1,871,584 + Psyr_1666 0.77 -0.2 1,871,584 + Psyr_1666 0.77 -1.0 1,871,585 - Psyr_1666 0.77 -1.2 1,871,593 + Psyr_1666 0.78 -1.5 1,871,593 + Psyr_1666 0.78 -1.0 1,871,594 - Psyr_1666 0.78 -0.5 1,871,594 - Psyr_1666 0.78 -0.7 1,871,703 + +1.1 1,872,023 + Psyr_1667 0.29 -0.6 1,872,023 + Psyr_1667 0.29 -1.9 1,872,023 + Psyr_1667 0.29 -1.7 1,872,023 + Psyr_1667 0.29 +0.7 1,872,023 + Psyr_1667 0.29 -0.2 1,872,023 + Psyr_1667 0.29 -2.4 1,872,024 - Psyr_1667 0.29 +0.1 1,872,027 + Psyr_1667 0.29 -3.3 1,872,027 + Psyr_1667 0.29 -0.8 1,872,027 + Psyr_1667 0.29 -2.3 1,872,027 + Psyr_1667 0.29 -1.4 1,872,028 - Psyr_1667 0.29 -1.4 1,872,028 - Psyr_1667 0.29 -0.9 1,872,061 + Psyr_1667 0.35 -0.5 1,872,114 + Psyr_1667 0.44 -1.0 1,872,397 + -0.6 1,872,397 + +1.7 1,872,397 + +0.3 1,872,397 + -0.7 1,872,398 - +1.1 1,872,398 - +0.4 1,872,436 + -0.7 1,872,437 - -0.0 1,872,437 - -0.4 1,872,506 + -1.4 1,872,506 + +0.3 1,872,506 + -1.0 1,872,631 + Psyr_1668 0.12 -0.9 1,872,631 + Psyr_1668 0.12 +1.1 1,872,631 + Psyr_1668 0.12 +1.7 1,872,631 + Psyr_1668 0.12 +1.0 1,872,632 - Psyr_1668 0.12 +0.1 1,872,632 - Psyr_1668 0.12 +0.1 1,872,632 - Psyr_1668 0.12 +0.7 1,872,632 - Psyr_1668 0.12 -0.0 1,872,653 - Psyr_1668 0.13 +0.6 1,872,908 - Psyr_1668 0.30 +1.4 1,873,021 + Psyr_1668 0.38 +0.0 1,873,061 + Psyr_1668 0.40 -1.7 1,873,061 + Psyr_1668 0.40 +0.3 1,873,061 + Psyr_1668 0.40 +3.9 1,873,062 - Psyr_1668 0.40 -1.2 1,873,062 - Psyr_1668 0.40 -0.6 1,873,142 + Psyr_1668 0.46 +0.3 1,873,142 + Psyr_1668 0.46 -0.3 1,873,143 - Psyr_1668 0.46 -0.3 1,873,197 - Psyr_1668 0.49 -0.1 1,873,197 - Psyr_1668 0.49 +0.5 1,873,224 - Psyr_1668 0.51 -1.1 1,873,224 - Psyr_1668 0.51 +0.5 1,873,256 - Psyr_1668 0.53 -1.4 1,873,265 + Psyr_1668 0.54 -0.9 1,873,265 + Psyr_1668 0.54 -2.3 1,873,266 - Psyr_1668 0.54 +1.3 1,873,436 + Psyr_1668 0.65 -0.1 1,873,436 + Psyr_1668 0.65 -2.0 1,873,437 - Psyr_1668 0.65 -0.9 1,873,437 - Psyr_1668 0.65 -0.6
Or see this region's nucleotide sequence