Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1210 and Psyr_1211 overlap by 4 nucleotides Psyr_1211 and Psyr_1212 are separated by 6 nucleotides Psyr_1212 and Psyr_1213 are separated by 2 nucleotides
Psyr_1210: Psyr_1210 - type III secretion protein HrcQa, at 1,367,316 to 1,368,032
_1210
Psyr_1211: Psyr_1211 - type III secretion protein HrpP, at 1,368,029 to 1,368,601
_1211
Psyr_1212: Psyr_1212 - type III secretion protein HrpO, at 1,368,608 to 1,369,054
_1212
Psyr_1213: Psyr_1213 - type III secretion cytoplasmic ATPase HrcN, at 1,369,057 to 1,370,406
_1213
Position (kb)
1368
1369
1370 Strain fitness (log2 ratio)
-2
-1
0
1 at 1367.656 kb on - strand, within Psyr_1210 at 1367.656 kb on - strand, within Psyr_1210 at 1367.656 kb on - strand, within Psyr_1210 at 1367.757 kb on + strand, within Psyr_1210 at 1367.757 kb on + strand, within Psyr_1210 at 1367.802 kb on + strand, within Psyr_1210 at 1367.802 kb on + strand, within Psyr_1210 at 1367.803 kb on - strand, within Psyr_1210 at 1367.803 kb on - strand, within Psyr_1210 at 1367.864 kb on + strand, within Psyr_1210 at 1367.864 kb on + strand, within Psyr_1210 at 1367.865 kb on - strand, within Psyr_1210 at 1368.362 kb on + strand, within Psyr_1211 at 1368.362 kb on + strand, within Psyr_1211 at 1368.363 kb on - strand, within Psyr_1211 at 1368.363 kb on - strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.455 kb on + strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.456 kb on - strand, within Psyr_1211 at 1368.609 kb on + strand at 1368.610 kb on - strand at 1368.716 kb on + strand, within Psyr_1212 at 1368.862 kb on + strand, within Psyr_1212 at 1368.863 kb on - strand, within Psyr_1212 at 1368.905 kb on + strand, within Psyr_1212 at 1368.906 kb on - strand, within Psyr_1212 at 1369.151 kb on - strand at 1369.151 kb on - strand at 1369.152 kb on + strand at 1369.206 kb on - strand, within Psyr_1213 at 1369.239 kb on + strand, within Psyr_1213 at 1369.239 kb on + strand, within Psyr_1213 at 1369.240 kb on - strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.241 kb on + strand, within Psyr_1213 at 1369.242 kb on - strand, within Psyr_1213 at 1369.242 kb on - strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.378 kb on + strand, within Psyr_1213 at 1369.379 kb on - strand, within Psyr_1213 at 1369.379 kb on - strand, within Psyr_1213 at 1369.450 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.457 kb on + strand, within Psyr_1213 at 1369.523 kb on + strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213 at 1369.524 kb on - strand, within Psyr_1213
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 1,367,656 - Psyr_1210 0.47 -1.2 1,367,656 - Psyr_1210 0.47 +0.9 1,367,656 - Psyr_1210 0.47 -0.2 1,367,757 + Psyr_1210 0.62 +0.4 1,367,757 + Psyr_1210 0.62 -0.3 1,367,802 + Psyr_1210 0.68 +0.8 1,367,802 + Psyr_1210 0.68 +1.5 1,367,803 - Psyr_1210 0.68 -0.9 1,367,803 - Psyr_1210 0.68 +1.2 1,367,864 + Psyr_1210 0.76 -0.1 1,367,864 + Psyr_1210 0.76 -1.0 1,367,865 - Psyr_1210 0.77 -0.3 1,368,362 + Psyr_1211 0.58 +0.8 1,368,362 + Psyr_1211 0.58 +0.5 1,368,363 - Psyr_1211 0.58 -0.4 1,368,363 - Psyr_1211 0.58 +0.8 1,368,455 + Psyr_1211 0.74 +1.0 1,368,455 + Psyr_1211 0.74 -0.1 1,368,455 + Psyr_1211 0.74 -0.5 1,368,455 + Psyr_1211 0.74 +1.6 1,368,455 + Psyr_1211 0.74 -0.1 1,368,455 + Psyr_1211 0.74 +1.3 1,368,455 + Psyr_1211 0.74 +0.8 1,368,455 + Psyr_1211 0.74 -0.4 1,368,455 + Psyr_1211 0.74 -0.5 1,368,456 - Psyr_1211 0.75 +0.3 1,368,456 - Psyr_1211 0.75 +1.0 1,368,456 - Psyr_1211 0.75 -1.2 1,368,456 - Psyr_1211 0.75 -0.3 1,368,456 - Psyr_1211 0.75 -0.4 1,368,609 + -1.9 1,368,610 - -0.4 1,368,716 + Psyr_1212 0.24 -1.5 1,368,862 + Psyr_1212 0.57 -0.6 1,368,863 - Psyr_1212 0.57 -0.3 1,368,905 + Psyr_1212 0.66 -1.6 1,368,906 - Psyr_1212 0.67 -0.0 1,369,151 - +0.2 1,369,151 - +0.8 1,369,152 + -1.1 1,369,206 - Psyr_1213 0.11 -0.8 1,369,239 + Psyr_1213 0.13 -0.1 1,369,239 + Psyr_1213 0.13 -0.0 1,369,240 - Psyr_1213 0.14 +0.1 1,369,241 + Psyr_1213 0.14 -0.7 1,369,241 + Psyr_1213 0.14 +0.2 1,369,241 + Psyr_1213 0.14 -1.7 1,369,242 - Psyr_1213 0.14 -0.8 1,369,242 - Psyr_1213 0.14 -0.5 1,369,378 + Psyr_1213 0.24 -0.3 1,369,378 + Psyr_1213 0.24 +0.7 1,369,378 + Psyr_1213 0.24 -0.8 1,369,379 - Psyr_1213 0.24 +0.2 1,369,379 - Psyr_1213 0.24 -0.5 1,369,450 + Psyr_1213 0.29 -0.1 1,369,457 + Psyr_1213 0.30 +0.1 1,369,457 + Psyr_1213 0.30 -0.3 1,369,457 + Psyr_1213 0.30 +0.1 1,369,523 + Psyr_1213 0.35 -1.2 1,369,524 - Psyr_1213 0.35 -1.1 1,369,524 - Psyr_1213 0.35 -0.9 1,369,524 - Psyr_1213 0.35 -1.2
Or see this region's nucleotide sequence